Disolución (uM) A (550nm)

2 0.065
6 0.205
10 0.338
14 0.474
18 0.598

Pendiente 0.0334 A 0.45

Ordenada 0.0023 D (uM) 13.40
R^2 0.9996

Curva de Calibración
0.7

0.6
f(x) = 0.033375 x + 0.00225
0.5 R² = 0.999598976970622
Abs (550 nm)

0.4

0.3

0.2

0.1

0
0 2 4 6 8 10 12 14 16 18 20
Disolución (uM)
 E (L / mol.cm)
480 nm 635 nm
cPd 3.55E+03 5.64E+02
cAu 2.96E+03 1.45E+04

A = E1xLxC1 + E2xLxC2
[cPd] mol/lt [cAu] mol/lt A nm
3.55E+03 2.96E+03 0.533 480
5.64E+02 1.45E+04 0.590 635

Inversa de la matriz
0.0002911322 -5.943112E-05
-1.132404E-05 7.1277183E-05

V (ml) 50 V (ml) 25
[cPd] mol/lt 1.20E-04 [cPd] mol/lt 2.40E-04
[cAu] mol/lt 3.60E-05 [cAu] mol/lt 7.20E-05