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Abstract
Commercial application of plant tissue culture started in USA with micropropagation of orchids in 1970s. It has
seen tremendous expansion globally from 1985 to 1990 in the number of production units as well as the number of
plants produced. With an estimated global market of 15 billion US dollars per annum for tissue cultured products,
even with exponential expansion in the industry, the demand far exceeds production, leaving enough scope for
expansion. This industry appears to be undergoing a pause in growth presently in developed countries as it is finding
difficult to remain cost–effective. In US, only half the production capacity is being utilized currently due to high
labour costs. In developing countries, with lower wage scales, plants are being produced at much cheaper rates.
Indian micropropagation industry, though a late starter by almost a decade, compared to its western counterparts,
has expanded exponentially from 5 million annual capacity in 1988 to 190 million in 1996. The facilities now
created are at par with the best in leading countries like the Netherlands and USA. To remain in profitable business
and to earn the much needed foreign exchange, Indian units need to judiciously mix steady revenue generating
items with unique speciality items based on demand in domestic and international markets.
*134385*
JS: PIPS No. 134385 BIO2KAP
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66
Geographic distribution
Analysis of data
The earliest commercial micropropagation laboratory
Source of technology in India was set up in Cochin Export Processing Zone
under the name A.V. Thomas & Co. It was followed by
Although Indian scientists are credited for reporting Indo-American Hybrid Seeds, Bangalore, Hindustan
several important breakthroughs in the area of plant tis- Lever Ltd., Mumbai and Unicorn Biotek, Hyderabad,
sue culture, unfortunately, very few commercial units in 1988. Regional distribution of Indian Commercial
in India have been set up with indigenous know-how. micropropagation units is given in Table 1.
Most of our commercial units prefer to seek the entire Geographical location of these production houses
technology package on a turn key basis and, in fact, is an important issue. The concentration of plant micro-
have been set up in foreign collaboration with M/S propagation units in Maharashtra, Karnataka, Andhra
Cultiss and M/S Green Tek, Holland; Microplants Ltd., Pradesh, Tamil Nadu and Kerala (Figure 1) perhaps
UK; Centre de Recherches Agronomiques, Belgium; could be because these regions
Plantex, Australia and Agrobio, France, etc., with buy- do not face extremes of temperature in summer or
back arrangements for the initial period. winter and their day and night temperatures (diur-
nal) do not differ very much, thus considerably
Product type bringing down energy (electricity) consumption for
air-conditioning and heating or cooling of green-
The existing commercial units can be classified by the houses,
product they offer such as
annual precipitation is sufficient
stage II or stage III cultures in vessels labour is relatively cheap and
in vitro rooted plantlets, or even the products can be speedily exported via Mumbai
‘hardened’ plants. or Chennai international airports.
The product type largely depends on whether the
Company is equipped with acclimatisation and green- Infrastructure created and performance analyses
house facilities. Indian industry generally produces
hardened plants for domestic market and in vitro rooted Though Indian tissue culture industry has been a late
plantlets for the export market. starter, the infrastructure created today matches with
the best in the world. In fact, Indian units have ber production. Godrej Plant Biotech Limited (earlier
an edge over their western counterparts as India is Unicorn Biotek) multiply trees and plants by apical
blessed with diverse agroclimatic zones, rich heritage and axillary meristems and somatic embryogenesis.
of germplasm, excellent scientific talent and availabili- Micropropagation protocols for 2 cultivars of straw-
ty of skilled as well as unskilled labour at much cheap- berry suitable for cultivation in tropical plains and
er rates. A.V. Thomas & Co., Cochin, were the first to conventional hilly regions have been developed and
put India on the modern tissue culture technology map over 2 lakh plants have been sold to farmers in Maha-
of the world in 1987. Since then, there has been an rashtra, Gujarat and Andhra Pradesh in 1992-93 and
exponential expansion in Indian tissue culture indus- 1993-94. Micropropagated banana, Chrysanthemum,
try, from 4 units in 1988 to 75 in 1996 as well as their rose and foliage ornamental plants are not only being
production capacity increasing from 5 million plants produced on a large scale but also being exported.
per annum in 1988 to about 190 million plants in 1996. Dhampur Sugar Mills, Dhampur and EID Parry, Chen-
Indo-American Hybrid Seeds (IAHS), A.V. nai are concentrating on the production of tissue culture
Thomas & Co., Harrisons Malayam Ltd., Godrej Plant raised virus-free plants of sugarcane. Vasantdada Sug-
Biotech Ltd., and SPIC have established ultramod- ar Institute, Pune, is involved in tissue culture work in
ern micropropagation facilities. The performance of sugarcane.
only a few leading commercial units is described here. SPIC Science Foundation, Chennai have intro-
Indo-American Hybrid Seeds and A.V. Thomas & Co. duced sex-typed female papaya plants in commercial
(AVT) have exported millions of tissue cultured plants tissue culture market for the first time in India. SPIC
to Europe as well as released several hundred thou- Agro Biotech Centre is standardising protocols in cof-
sands of banana and cardamom plantlets to farmers in fee, banana, potato, sunflower, rose, Gerbera, orchids
the domestic market. AVT have developed a cardamon and tree species such as Calistemon, Simaruba, jack-
variety with yield of 250 kg ha 1 as compared to the fruit, mango and datepalm. Harrisons Malayalam Ltd.,
normal 70 kg ha 1 and can be cropped in two years Bangalore have developed protocols for micropropa-
instead of the usual three years. AVT have also devel- gation of over 30 crops and all these plants are being
oped high glucovanillin producing and early flowering commercially produced for domestic and export mar-
vanilla plants through tissue culture. Cost effective kets. Protocols for cashew, mango, teak, rosewood and
micropropagation system has also been developed in tamarind are being developed.
tea and black pepper and plants are currently under field National Agricultural & Scientific Research Foun-
trial. Being a plantation based company, AVT absorb a dation, Calcutta, is engaged in large scale production of
considerable portion of tissue culture products for their Musa, Spathiphyllum, Anthurium, Elettaria, Platyceri-
own use (Mohanty and Sastri, 1994). um and orchids and has developed protocols for micro-
IAHS has won several contracts from the Nether- propagation of tea, jojoba, lilies, Gladiolus, Gerbera,
lands, UK and Denmark to develop new plant vari- Ficus and Philodendron (Agarwal et al., 1994).
eties by somatic hybridisation, selection of somaclonal
variants, endosperm culture, in vitro pollination, and Plants produced
embryo rescue method, in combination with whole
plant breeding. IAHS is capable of offering disease Indian units are currently producing fruit crops, forest
screening and cleaning services for 40 viruses, 2 trees, ornamental (foliage plants, flowering plants),
viroids and several bacterial, fungal and mycoplas- vegetable crops and plantation crops (Table 2).
mal diseases for 19 ornamental and 26 horticultur- An analysis of plants micropropagated by Indian
al/agricultural crops (Prakash, 1990). They had export- industry shows that ornamental plants are the major
ed flowers worth Rs. 14 million to Holland, Denmark items being produced, in line with the international
and UK in 1991. trend (Figure 2).
Beena Nursery (P) Ltd., Trivandrum, offers high
quality and disease-free plantlets of orchids suitable for Market
the cut flower industry. Hindustan Lever Ltd., Mumbai
was involved in clonal multiplication of high-yielding The market for tissue culture plants is huge, ever
cultivars of plantation crops such as cardamom through changing, highly competitive as well as relatively
meristem culture, virus-free microtubers of potato and volatile and, therefore, needs proper planning, It is
had designed a bioreactor to scale-up the microtu- estimated that there is a world market potential of 15
Table 2. Plant species being currently micropropagated by Commercial tissue culture units in India.
FRUIT CROPS
Harrisons Malayalam Ltd. Banana, *Citrus, Grapes, Papaya, Pomegranate, Strawberry
Godrej Plant Biotech Ltd. Banana, Pineapple, Strawberry
IAHS Banana, Cherryapple, Peach, Pineapple, Strawberry
Nath Seeds, Bio Tissue Labs, Microplantae, Kumar Gene Tech, Banana
Maharashtra State Seed Corporation, National Agricultural &
Scientific Research Foundation & SIV Industries Ltd.
A.V. Thomas & Co. Banana, Pineapple
SPIC Banana, Datepalm, Mango, Papaya (Female)
Enzo-chem Papaya
Agrigene International Apple
Microplantae, Indrayani Biotech Strawberry
Saubhagya Kalpataru Pvt. Ltd. Banana, Strawberry
FOREST TREES
Harrisons Malayalam Ltd., Tata Tea Ltd., Thapar Corp. R&D Eucalyptus
Centre, SIV Industries Ltd.
IAHS Teak, Kadamb
SPIC Calistemon, Eucalyptus, Jackfruit, Simaruba
Godrej Plant Biotech Salavadora
Saubhagya Kalpataru Pvt. Ltd. Bamboo
ORNAMENTALS
Harrisons Malayalam Ltd. Foliage plants
Aglaonema, Calathea, Cordyline, Diffenbachia,
Ficus, Philodendron, Spathiphyllum, Syngonium
Flowering plants
Alstroemeria, Anthurium, Callalily, Carnation,
Cestrum, Chrysanthemum, Gerbera, Impatiens,
Limonium, Rose (Miniature)
IAHS Alpinia, Anthurium, Calethea, Callalily,
Chrysanthemum, Cordyline, Croton, Diffenbachia,
Dracaena, Eryngium, Ficus, Freesia, Gardenia,
Geranium, Gerbera, Gladiolus, Hydrangea, Iris,
Impatiens, Lilies, Maranta, Orchids,
Philodendron, Rose, Spathiphyllum, Syngonium
SPIC Anthurium, Calathea, Cordyline, Gerbera, Maranta,
Orchids, Rose, Syngonium
Godrej Plant Biotek Anthurium, Calathea, Ficus, Lily, Ornamental
Bamboo, Spathiphyllum, Syngonium
Agrigene International Ficus, Spathiphyllum
Nath Biotech Ficus, Philodendron, Syngonium
A.V. Thomas & Co. Chrysanthemum, Gerbera, Lily, Orchids, Rose
Cadilla Laboratories Ltd. Calathea, Lily, Spathiphyllum, Syngonium
Kalpataru Irrigation Systems Ltd. Lily
Rallis India Carnation, Orchids
COSTFORD promoted Unit Dendrobium (Orchid), Phaelenopsis
Phytoclone Dendrobium
Pocha seed, Khoday Biotek Ornamentals
Padamjee Paper Mills, Florida Plant Cultures, Green Laboratories Foliage plants
VEGETABLE CROPS
Harrisons Malayalam Ltd. Potato, Asparagus
SPIC Potato
Proagro Brassica napus, Brassica juncea, Lycopersicon
esculentum (tomato)
Frontier Biotech, Hindustan Lever Ltd., Kalyani Agrotech, AgroIndia Microtubers of Potato (Solanum tuberosum)
Biotechnologies
IAHS Asparagus, Cauliflower, Cabbage, Tomato
Agrigene International Asparagus officinalis (exotic vegetable)
PLANTATION CROPS
Harrisons Malayalam Ltd. Cardamom, Sugarcane, Vanilla
IAHS Cardamom, Coffee, Ginger, Miscanthus, Sugarcane
Turmeric, Vanilla
Tata Tea Ltd. Coffee, Tea
SPIC Cardamom, Vanilla
A.V. Thomas & Co. Cardamom, Ginger, Turmeric, Vanilla, Black Pepper, Tea
Bush Boake Allen Vanilla
Dhampur Sugar Mills, EID Parry Ltd., Rallis India Ltd., Vasant Dada Sugarcane
Sugar Institute, Frontier Biotech., Western India Tissue Tech Ltd.,
Agri India Biotechnologies, Saubhagya Kalpataru Pvt. Ltd.
Kalpataru Irrigation Systems Ltd. Coffee, Tamarind
National Agricultural & Scientific Research Foundation Cardamom
*Plant (name in bold) being commercially produced by more than one unit.
leaving enough scope for expansion. Twenty four units protocol with rapid multiplication rates, demand in
are producing a large number of ornamentals. A few domestic and international markets as well as build
vegetable crops and some plantation crops like car- good market reputation by delivering quality products
damom, vanilla, sugarcane and ginger are also being on time. The micropropagation industry is particularly
multiplied (Table 2). Indian units need to strike an well suited for a country like India as it is environment-
intelligent balance between product diversity as well friendly and labour-intensive. It can really solve our
as quantity and should try to introduce novelty and unemployment problem to a reasonable extent, only if
exploit commercial potential of endemic ornamentals planned judiciously.
as new entrants. In fact, production by micropropa-
gation units is market-driven and based on advance
orders. The production of large number of ornamental
and horticultural plants reflects their demand by con- Conclusions
sumers as well as their willingness to pay the higher
cost. Blessed with diverse agroclimatic zones and availabil-
Globally, the floriculture market alone is supposed ity of both skilled manpower as well as labour at cost-
to be of Rs. 750 billion level. While considering cost- effective rates, combined with liberalised Government
benefit ratio for identification of products, social and policies and incentives provided, Indian tissue culture
environmental aspects whose monetary value cannot industry, though a little late starter, is all set to have its
be estimated, should also be given due consideration. presence felt on the international scenario and make a
With the liberalised industrial policy, incentives dent in the national economy by earning considerable
provided by the Government and demonstration efforts foreign exchange on a recurring basis.
made by the commercial units, tissue culture products
of our units are fast capturing domestic market as well
as increasingly becoming popular in international mar-
kets. Looking at the brighter prospects of the industry, Acknowledgements
Government of India had fixed export target to Rs.
1300 million for cutflowers, seeds, live plants and tis- The authors are grateful to the commercial microprop-
sue culture products in 1995-96 (Kumar, 1994). agation units for kindly providing valuable informa-
tion. Mrs. Suman Govil, is grateful to the Department
of Biotechnology, New Delhi, for granting her study
Recommendations
leave for carrying out this study.
The widespread application of in vitro propagation
depends on its cost-competitiveness and is profitable
only when there is an associated advantage over con-
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