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Procedure
Solutions:
Notes
1. Although making your own solutions is much cheaper, you can also order a kit
containing all the solutions premade from Carolina Biological. Enough reagents
are provided for ~ 40 mini-preps. Plasmid DNA Isolation Reagent System
(catalog no. L2-21-1310).
2. In the classroom, we have been able to complete the isopropanol step (step 8)
by the end of the class period (50 minutes). The next day, the rest of the
procedure can be completed, and the next experiment, such as a restriction
enzyme digest, can be set up.