Calibration Certification Procedure

Ultra Pro 80 Ultracentrifuge

Revised - 5/1/98
1. General

Successfully completing the Preventive Maintenance Checklist and Calibration

Certification Procedure as outlined will ensure that the instrument is properly maintained
and calibrated to SORVALL specifications.

2. Equipment Required

• Digital Multimeter
• Temperature Meter w/probe
• Stopwatch
• Centrifuge Analyzer
• Ultra Pro 80 Service Manual
• Centrifuge Certification Service Manual

Note: Check calibration due date on all test equipment before starting certification.

3. Documentation Required

• Preventive Maintenance Procedure and Checklist (Ultra Pro 80 Service Manual)

• Calibration Certification Procedure and Form
• Certification Label
• Certificate of calibration for each piece of test equipment used to perform the
certification
• Training Certificate

Preventive Maintenance Check

• Perform Preventive Maintenance checks as outlined in the SORVALL Ultra Pro 80

Service Manual to ensure the instrument is in good working order without performing
any calibrations.
• Complete the Preventive Maintenance Checklist. (calibration data will be filled in
after Certification)

Calibration Certification

• SPEED

Install a rotor, set the speed to 2000 rpm and start the centrifuge. When the speed has
stabilized, measure the actual speed with an external tach (centrifuge analyzer set on
Portach 3, or frequency on multimeter where speed = frequency multiplied by 3.75).
Record measured speed and displayed speed in the speed section of the certification form.
Set the speed to the top speed of the installed rotor and after stabilization, record
measured speed and displayed speed in the speed section of the certification form.
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Determine if the speeds recorded meet the specifications on the form. If the specifications
are met, proceed to the next section. If the specifications are not met, make the necessary
repairs or recalibrations using the appropriate sections of the Service Manual, repeat the
above speed checks and record the data in the “after calibration” column of the
certification form. Initial the speed section.

• TEMPERATURE

Prepare samples for the rotor being used. Place the samples inside the rotor and precool
the samples, rotor and centrifuge chamber to 4°C. Run the centrifuge for a minimum of 1
hours at 4°C set temperature and a set speed equal to the top speed of the installed rotor.
Stop the centrifuge and take the sample temperature using your temperature meter and
probe. Record the measured temperature and displayed temperature in the temperature
section of the certification form. Determine if the temperature values recorded meet the
specifications on the form. If the specifications are met, proceed to the next section. If the
specifications are not met, make the necessary repairs or recalibrations using the
appropriate sections of the Service Manual, repeat the above temperature check and
record in the “after calibration” column of the certification form. Initial the temperature
section.

Note: A suggestion to save time would be to ask the customer to set up the samples and
centrifuge to 4°C (precool) and start a hold run, the evening before, which you can
shut down when you arrive and take the sample temperature. The time to precool
samples, rotor and chamber to 4°C from an ambient of 25°C, could take as long as
12 hours.

• TIME

Using a stopwatch, check the timer by setting the time for a 10 minute run. Start a manual
vacuum first. When the vacuum level achieves the medium range (<750µ), start the
centrifuge and measure from the time the start button is pushed until the time display
reads 0 and the centrifuge switches to stop. Record the measured time in the time section
of the form. Determine if the time value recorded meets the specifications in the form. If
the specifications are met, proceed to the next section. If the specifications are not met,
make the necessary repairs or recalibrations using the appropriate section of the Service
Manual, repeat the above time check and record in the “after calibration” column of the
certification form. Initial the time section.

Documentation

After filling in the certification information complete the following documentation:

• Log test equipment data on calibration certification form.

• Review P.M.checklist and calibration certification form to ensure that all data and
required information has been recorded properly.(including your signature and date)

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• Fill out a certification label and place it on the right side of the centrifuge where
visible or at a location selected by the customer.
• Ask the customer to sign the calibration certification form and place a copy in the
customer’s Certification Record Book for that centrifuge.
• Place copies of the P.M.checklist and Field Service Report in the same Record Book.
• Provide copies of your training certificate and test equipment calibration certificates if
requested by the customer.

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