ONE UC favorita Feelevaterat VIDYASAGAR UNIVERSITY ANSWER BOOKLET (For Online Exam) For all Final Year/Semester Examinations 2020 To be filled by the Candidate Name of the Examinations: B-Sc. Honours Examination, 2020 Year of Examinations 2020 USIN (This number is For VU PG Students only) Candidate’s Rol: 14} G1 2 No: \Fo 33 Registration Taig a5e | Yar 2017 = 5018 Subject Physica | fater |DSEX3 (T+P) with | Nano Materials and Applications jiame (theory + Practica?) Half (any) = Instructions ‘This Answer Booklet is Security. marked with your identity only. Sharing it with other examinees may lead to cancellation of your éaiididature. However, print / photocopy of the same blank answer booklet may be used for all papers of your examinations. Second page of the aiswer booklet may be used as additional sheet by printing / photocopying it ‘Write your Answer here Part Az Nano Materials ound Applications Theory) 3, Working Principle of TEM in G@)Teansinissien Electron Microscopy (TEM) tmvolves a high eneryy electron beam, emitted from an eletran i ‘Downloadod from Vidyasagar Universiy, Downloaded rom IP Address: 202.142.71.48 et 02-10-2020 02:60:93 AM Scanned with CamScanner(Write your Answer here) gun, te transmit thro in port trans ‘information about the inner strudure GY) This bansmitted ehetron heam ts then hk a speetmen that is it to clectrons and carries the of specimen reaches the imaging system of the microscope and the spatial varic Dac a, thts Doforontton’ Cine “amage.”) is then magnified by a series of electro- magnetic lenses umtil it is recorded by hitting « fluorescent” sereen, photographic ,or..Light sensiti Sentor such as a. charge-coupled device (CCD) camera. The image detected by te CCDimay be dueplayed in real-time 0 a monitor or 6% Gi) TEMs work tre ay as SEMs except] the transmitted post of the electrons is projected ott orto o phosphor seven for the User to see. e Various steps of the working of TEM axe as follows (see-fig- 1, neat page) oO Firsty, the electron gun produces a sbream of monschromatic. eleetrors, which is then focused to al Small; thin; coherert beam ly the. use of condenser 15s The beam is westsictad by the conckrser aperttye, (Usialty user selectable), knocking out high angle. elation Cthose. far from tre. optic axis, the dotled Line down thy centre. Gi) Thereafter, the beam strikes the. specimen pasts of it ave transmitted. This trans mitted postion # focused by the objective lens ito an image. Fage- a Downloaded fem Vidyasagn Universly. Domloaded for IP fekeess = 202 142.7148 at 02-10-2020 0943.58 AM vn 017-2018, Scanned with CamScannerWrite your Answer here) ba-- ~ Electron Source. (nny EN D-- — — Phosphor Seseejny Ligud. shows. Schematic diagram of a TEM Page 3 * gg ‘Downloads tom Vidyasagar University, Coarionde fom P Adress: 202.142.7148 at 02-10-2920 02 43.38 0A Scanned with CamScanner‘Additional Sheet optione® (write your Answer here) Git) Objectie and Sle Selected Area. metal apertures can restrict the. leam;s whereos the objective aperture, enhances the. contrast by Sackieg out high-angle diffracted cketrons, whereas tha. selected area aperture enables the userto examine ‘the periodic diffraction of electrons by ordered arvangements of atom in the sample. (0) The image is tren passed down te, colusnin through “the. intermediate and peejectar lenses, being enlarged all the way Q) Then the image pbrikes the phosphor gereen are} tight is generated, allowing uses to bee the image. ® The darker areas df then tmage represents those. areat of the Sample: thal fewer electrons were tranmilted through. (they are chides axd denses)e * The Lighter areah Of the image represent these areas of the sample. that move electrons uere transmitted theough (they are thinner or less dense. “A stenplified repsesertation of tne principles of the tmnga image. formation ts given in 92) Ennesat pega a Page-4 1210206-2017-2018 ‘Dopnbaded rm Vidyatagar Unvesty.Desmiosded tom IP Aue 202.142.7148 at02 102020 09 43:38 AA Scanned with CamScanner‘Additional Sheet (Write your Answer here) optieet. ans Object plane object Lens Back fecad plane (diffraction pattern) Flane fig @) Shows 2 Semplified representation of the. rasy perth 7 leading to the first intermediate image in the. tmaga plane. of the object lens. Electrems, which come fiem the condones System of the TEM, are scattered by te sample, bituated i: the object plane of “the object lems « Electron sation! in the. same dircetion are. focused im the back lan€,cnd ap a. Teult, a diffraction pattern is formed ‘there . Electsonk ing from tre some point: of ‘tre object are focured a acu plane. In the TEM, the first intermediate image (s magnifie by furtrer lemes (projective system). Page-S c Downloaded rom Vidyosagar Univesity, Dowloaded ro IP Adds : 202.142.7148 at 0240-2020 09 43°38 AM 1210206-2017-2018 Scanned with CamScanner‘Additional Sheet (Write your Answer here) Paar B* Nano Materials ond Application» (Practical) 7. Explaining the procedure of chemical, systthesis of any Robe metal nanoparticles —— Nobe metals are metals that ave resistant to corrosion amd oxidation in moist ai”, ¢g-)Ru,Rhy Pd, Ag. Os, Ir, Pt and Au. Ly We with in particular, take’ gold (Au) in Tis Cah» © Aim: Synthesis of Geld cimbparticLe. © Chemicads @Chlordtiueric Acid CHAuCLy ) Wyre fam Citrate ( CcH,0, Nag) Cy’ Double. dictitted water. © Equipments: (i) Round bottom flask (i) Magnetic Stivrer cum hedles Gi) Optica absorption mpactremeter (~250 tp 10m) © Proceduse ? Tt is given in the form of flewdurh flow-chart in te next page. Fage-6 “et GE oanoeded fom Vidyesager University. Doaioadad fom IP Address 202.142.7148 a 02-10-2020 43:38 AM Scanned with CamScanner1Oml of 0-002M aqueous HAUCly- > H,0 A IK —§ Stir for IS min ot 80°C lO-mL of O-01M aqueous sodium (nitvale Clear solution tutes iat ita ved colour andicoting fermabion of gold nanopasticles of. procedure : Mts + Reductant ——> Nonoparticle Where Me gold (Au) Reductant = Citrate @) Synthesis of gold colloid in water staocti ag ier a solution of hydrogen tebrachloroaurete (MALCTy) “eA (Dosnicaded fom Viyasagar Unversty, Downloaded fom IP Addiess 202.142.7148 a 02-10-2020 09 49.9 AM Scanned with CamScanner‘Additional Sheet (Write your Answer here) and a gelution of trisodium citrate (Nay 4.) Gi) Gold colloids forms with nanoparticles 10-20nm * ize Gai) Ty the veachio, the citrate acts as a Neak Feduetng agent: (vedeveing Auclt to Au.) and as a stabilizer. Gu) A Tayer of eltrate anions adearbe arourd ach nanoparticle and prevents these. from aggregating: the anions electrostatic vepulsisn keeps the nanoparticle, éeperated - tas G) Tn this state, the eolvoik wuby— ved Lor mages ved). The distinctive colours of colloical gold are dur! | to & phenomenon known ar “Surface. Planmon Resonanke. appears web Fage~@ — uo "Houma rom Vidyesag4r Univsty,Doumioaded from I Addees 202.142.1148 et 02 19-2020 0949.29 AM Scanned with CamScanner