ABSTRACT

COMPARATIVE ANALYSIS OF ANTIMYCOTIC ACTIVITY OF ALOE VERA

AND GARLIC AGAINST Candida albicans, C. Y. 2011

JOANNE PENAREDONDO ALUZAN, University of Nueva Caceres, City

of Naga, 2011.

Keywords: aloe vera extracts, garlic extracts, Candida

albicans, antimycotic activity, agar-well
diffusion method

This study was conducted to evaluate and compare the

antimycotic activities of aloe vera and garlic against

Candida albicans at different levels of concentration, C. Y.

2011. Specifically, it answered the following questions: (1)

What is the effect of aloe vera and garlic extracts in

inhibiting the growth of Candida albicans? (2) What are the

effects of aloe vera and garlic extracts against Candida

albicans using 25%, 50%, and 75% concentration levels? (3)

Are there significant differences on the effect of aloe vera

and garlic at different levels of concentration in

inhibiting the growth of Candida albicans?

This study made use of the experimental method of

research specifically the agar-well diffusion method in

investigating the effectiveness of aloe vera and garlic

extracts at different levels of concentration namely: 25%,

50%, and 75% against Candida albicans. The antimycotic

effects of aloe vera and garlic against Candida albicans

were quantitatively and qualitatively analyzed by the

presence or the absence of zones of inhibition. The prepared

treatments were as follows: AT1 (75% AVE), AT2 (50% AVE), AT3

(25% AVE), GT1 (75% GE), GT2 (50% GE) and GT3 (25% GE).

Fluconazole was used as a positive control and T0

(untreated) as the negative control for the study.

The major findings of the experiment are as follows:

(1.1) Aloe vera with a mean diameter zone of inhibition of

1.45mm was found to be inactive as antimycotic agent. (1.2)

A mean diameter zone of inhibition of 14.56mm using garlic

has an active effect on the growth of Candida albicans.

(2.1) T1, 75%; T2, 50% and T3, 25% concentration levels of

aloe vera failed to lessen the growth of Candida albicans.

(2.2) T1, 75%; T2, 50% and T3, 25% concentrations levels of

garlic extracts effectively reduced the growth of Candida

albicans. (3.1) The computed F-value of 1,983.44 at 5% level

of significance, using Two-Way ANOVA was greater than the

tabular value of 18.51 shows statistical difference of aloe

vera and garlic in their antimycotic effect. (3.2) The

computed F-value of 2.992 which is lesser than the tabular

value of 19.00 shows no statistical difference in the

antimycotic effects of varying concentrations of aloe vera

and garlic.

Laying these outcomes, the major conclusions are: (1.1)

Aloe vera has a very low antimycotic effect thus it is not

effective in inhibiting the growth of Candida albicans.

(1.2) Garlic reveals superior antimycotic activity in

reducing the growth of Candida albicans. (2.1) Aloe vera

extracts at T1, 75%; T2, 50% and T3, 25% concentration levels

were observed to be inactive, thus has a minimal possible

effect to the growth of Candida albicans. (2.2) The growth

of Candida albicans was maximally inhibited at T1, 75%; T2,

50% and T3, 25% concentrations levels of garlic extracts,

thus garlic holds excellent potential as antimycotic agent.

(3.1) There is a significant difference in the effectiveness

of aloe vera and garlic in inhibiting the growth of Candida

albicans. (3.2) There is no significant difference among the

varying concentration of aloe vera and garlic extracts in

reducing the growth of Candida albicans.