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Investigation of the effect of mebavin on the adjuvant arthritis showed that this water-
soluble derivative of gossypol (preparation dosage of 100 µ g/kg) possesses an anti-
inflammatory activity comparable with that of prednasolone.
Introduction
Experimental Methods
Experiments were conducted half on 104 Vistar rats, weighing 200-250 grams, and half
on 150 inbred mice, 3-4 months of age of line SVA with final body mass of 18-25 grams.
Maintenance of animals accomplished in accordance with regulations, adopting European
conventions for protection of experimental animals, utilization of experimental and its
series (Straβ burg, 1986).
Adjuvant arthritis (AA), complete adjuvant (PAF) provided for animals, with the help of
our friends at the firm Sigma (U.S.A.). For rats with AA, were given a one-time
subcutaneous shot of 1 milligram PAF introduced at the base of leg, maintenance of 5
milligrams BCG per milligram (1milligram/ rat). For mice in AA study, were given
single introduction of PAF in hind leg (0.2 milligram/mouse).
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1 Department of Experimental Medicine and Pathological Anatomy (Director- Professor
M.D. Urazmetova) State Science Center of Emergency Medicine, Republic of
Uzbekistan, Tashkent, 700107, Farhod Street 2
Study of effects of mebavin, prednasol, and also their combinatory utilization on animals
with AA. Prepare animal’s intake every day in afternoon inducing pathological processes
until end of experiment with following dosages: mebavin- 100 milligrams/kilogram/day
internally, prednasolone- 30 milligrams/kilogram/day subcutaneously. Since the object
of combinatory therapy appears to achieve therapeutic synergistic effects and reduces
toxicity, dose daily from preparation, reduce by 2 times and working out calculation of 50
and 15 milligrams/ kilogram. Concerning toxicity of combinatory application judging by
their affect on general weight and mortality.
What is apparent from their tabulation, in mice with AA without treatment, average size
lesion on hind leg compiled 323.3 conditional units during all days of observation were
statistically reliably increased compared with the other group of animals, with a small
pronouncement expressed on 14-28 days of illness – 299.7 +/- 6.2 ÷ 377.1 +/- 5.5
conditional units (P1< 0.05).
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Effectiveness of mebavin, prednasolone and their combinatory use, by degree of swelling
(%), hind extremity of strain CVA mice with adjuvant arthritis (estimated by “+++”).
↑AA- adjuvant arthritis; M- AA + prednasolone; MP- AA + mebavin + prednasolone.
Over abscissa- days observed; over ordinal- degree swelling, %.
Degree of swelling of hind leg of rats with adjuvant arthritis (AA) with treatment with
mebavin, prednasolone, and also their combinatory application.
Margin of Period of Treatment Number of Animals Average Size
Inhibition Animal Health Hind Extremity
of rat, condition
of individuals
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↑Comments. Differences in reliability of relative evidence: * - Animal health (control)
(P1<0.05); **- Rats with AA for corresponding period of treatment (P2<0.05).
Visual observation over condition of extremity of animals (7-21 days) showed that it was
developed by 100% of all mice. Average showed maximal swelling, estimated by “+++”
membership 87.5%, with highest indicator on 21st day (95%), figure.
In this series the most depression that is able to be achieved by combinatory application
of mebavin and prednasolone, which indicates synergism acting between the drugs.
Conclusion
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Literature
Received 12.08.02
Investigation of the effect of mebavin on the adjuvant arthritis showed that this water-
soluble derivative of gossypol possesses an anti-inflammatory activity comparable with
that of prednasolone.
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