Matthew Harold Fountain

1413 Van Steffy Avenue

Wyomissing, PA 19610
(207) 713-4413
mf57884a@westpost.net
http://www.linkedin.com/in/matthewfountainbiotech
Position:
BIOTECHNOLOGY LABORATORY PROFESSIONAL
Skill Set:
* Academic, clinical & quality laboratory experience of 11+ years
with GLP/GMP of various learned methodologies.
* Hypothesis testing and practices.

* Molecular Biology & Biochemistry: DNA extraction & cloning, RNA

extraction, quantification & reverse transcription,
non-quantitative and quantitative PCR. Protein Purification &
Immunoblotting.
* Large-scale relative gene expression assaying - combining
microarray data with RT-qPCR using Primer Express designed
primers.
* Standard cell culture practices (E. Coli, yeast, plant cells -
including those with non-differentiated embryonic
characteristics).

Quality Control methodology and practices.

* Analytical Chemistry, Organic Chemistry, Molecular Biology,
Microbiology
* Validation & Functional Testing Protocols

American Society for Quality - Certified Quality Technician (ASQ CQT).

Routine use and maintenance of instruments and equipment.

Data organization, analysis and presentation using Microsoft Office.

Work History:
DiagnoCure, Inc., West Chester, PA 06/08-07/09
LABORATORY TECHNICIAN
* Performed clinical laboratory work (Histology, biological sample
 processing, RNA extraction & quantification, RT and qPCR) for
detecting levels of an expressed RNA marker used to stage colon
cancer as an evaluator of potential post-surgical cancer
treatment.
* Performed Quality Control functional testing of supplies,
outsourced reagents, in-house reagents & QC monitoring equipment.
* Prepared testing equipment for QC monitoring.
* Assisted QC with data management and SOP protocol development.
* Manufacture of in-house reagents with inventory tracking.
* Successfully assisted laboratory in achieving CLIA certification.
Bates College, Chemistry Dept., Lewiston, ME 07/05-01/07
LABORATORY RESEARCH ASSISTANT, 18-month appointment
* Determination of experimental binding constants of various
bound/unbound RNAs to ss found among a protein complex.
* Assisted investigation of how an activated bound RNA/protein
complex regulates transcription in E. coli as a response to
environmental stress.
* Responsible for laboratory maintenance, ordering supplies and
assisted undergraduate students with projects.

Purdue University, Biochemistry Dept., W. Lafayette, IN 08/01-12/04

GRADUATE RESEARCH ASSISTANT
* Utilized microarray data to develop a thesis study screening for
hormone-responsive and/or chromatin remodeling factor-dependent
genes by qRT-PCR that exhibit decreased or increased expression
during various growth stages of the plant model organism
Arabidopsis thaliana.
* Secured and cataloged seed lines with mutants of various genes of
interest from the study and designed primers for detection of
inserts.
Indianapolis Water Company, Indianapolis, IN 04/96-06/01
LABORATORY TECHNICIAN I
* Water quality testing of samples from various points of production
& distribution systems.
* Wet Analytical Chemistry, Spectrophotometry, Electrochemistry,
solids & total dissolved solids analysis.

Maintenance of Quality Control laboratory including manufacturing of

testing chemicals, cleaning & calibration of testing equipment and
preparation of sample containers.

Test data organization for reports and records.

Trained in microbiological scoring and testing for weekend rotation.

Education:
Master of Science (Thesis) - Biochemistry, December 2007
Purdue University, West Lafayette, IN
Ross Fellowship recipient for first-year students.
Thesis Abstract, sample table, 3-publication list available upon
request.
Bachelor of Science - Biology, May 1995
Tufts University, Medford, MA
Membership:
American Society of Quality, March 2009 (ASQ CQT - 03/10).
References:
Four references available upon request.