PRODUCT:
Atlas® Human Hematology/Immunology Array
CATALOG No. Description
634507
LOT NUMBER
Specified on product label
STORAGE CONDITIONS
• Store Atlas Arrays at –20°C sealed
in a plastic bag away from light.
• Store Express Hyb™ Hybridization
Solution and Atlas NucleoSpin
Extraction Kit at room temperature.
Before using hybridization solution,
dissolve any precipitate with gentle
warming.
• Store Control Poly A + RNA at
–70°C
• Store all other reagents at –20°C
SHELF LIFE
1 year from date of receipt under proper
storage conditions.
SHIPPING CONDITIONS
Boxes 1 & 2: Blue ice (4°C) or Dry ice
(–70°C)
Box 3: Room temperature
FOR RESEARCH USE ONLY CD-ROM (PT3593-CD)
QUALITY CONTROL DATA
See back of page.
APPROVED BY: _________________________
(PA2Y194)
The Atlas® Human Hematology/Immunology Array
includes 406 human cDNAs, nine housekeeping
control cDNAs, and negative controls immobilized in
duplicate dots on a nylon membrane. Two identical
arrays are included, along with reagents for synthesizing
radioactively labeled cDNA probes. A transparent
Orientation Grid is also provided to allow identification
of cDNA's corresponding to the positive hybridization
signals. See the enclosed CD-ROM for functional
classes and complete gene representation on this
array.
Package Contents
Box 1:
• 2 Atlas Human Hematology/Immunology
(10 ng of cDNA/dot)
• 12 µl Hematology/Immunology CDS Primer Mix
• 1 Blank Nylon Membrane(s)
• 1 Orientation Grid(s) (PT3140-2)
Box 2:
• 120 µl 10X dNTP Mix (for dATP label)
(5 mM each dCTP, dGTP, dTTP)
• 600 µl 5X Reaction Buffer
• 15 µl MMLV Reverse Transcriptase
(100 units/ µl)
• 120 µl DTT (100 mM)
• 60 µl 10X Termination Mix
• 2.5 µg Human Placenta Control Poly A+ RNA
(0.5 µg/µl)
• 65 µl Human C0t-1 DNA
• 1.2 ml Deionized H2O
Box 3:
• 100 ml ExpressHyb™ Hybridization Solution
• 1 Atlas NucleoSpin® Extraction Kit
Other
User Manual (PT3140-1)
Atlas® Human Hematology/Immunology Array Cat No. 634507

QUALITY CONTROL DATA

1. The size and identity of all cDNAs were verified by PCR amplification, agarose/EtBr gel electrophoresis and
sequencing.

2. The Control Poly A+ RNA was electrophoresed on a formaldehyde/agarose EtBr gel. The pattern observed was that
expected for human placenta poly A+ RNA.

3. A biotinylated cDNA probe was synthesized using the control poly A+ RNA according to the Atlas SpotLight™ Labeling
Kit (Cat No. 634803) User Manual or a radioactively labeled probe was synthesized using the Control Poly A+ RNA
according to the Atlas Array User Manual. This probe was hybridized to the array, and the expected hybridization
pattern was observed.

Notice to Purchaser
Clontech products are to be used for research purposes only. They may not to be used for any other purpose, including, but not limited to, use in drugs,
in vitro diagnostic purposes, therapeutics, or in humans. Clontech products may not be transferred to third parties, resold, modified for resale, or used to
manufacture commercial products or to provide a service to third parties without written approval of Clontech Laboratories, Inc.
The Atlas® Array products sold by Clontech® are for research purposes only. Certain DNA sequences included on the Atlas Arrays may be covered by
U.S. patents. Presently, it is not clear under U.S. laws whether commercial users must obtain licenses from the owners of the rights to these U.S. patents
before using Atlas Arrays.
These products and the sequences of the polynucleotides thereon are intended to be used for the purchaser’s own internal research purposes only and
may not be used for drug diagnostic purposes or human use.
DISCLAIMER OF RIGHTS
No license is conveyed with the purchase of this product under U.S. Patents Nos. 5,804,375, 5,994,056 and 6,171,785, and
corresponding patent claims outside the U.S., relating to the 5’ nuclease and dsDNA-binding dye processes. Further information on purchasing licenses
may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Clontech, the Clontech logo and all other trademarks are the property of Clontech Laboratories, Inc.
Clontech is a Takara Bio Company. ©2006
PA2Y194 www.clontech.com Clontech Laboratories, Inc.