Dedicated professional offering successful career in scientific research and lab

oratory management capacities within the areas of biotechnology. Experienced in

DNA sequencing projects. Adept in performing and upholding the integrity of labo
ratory experiments; compiling and analyzing data; drafting reports on research f
indings; and presenting data to management and associates.
EDUCATIONAL BACKGROUND
Bachelor of Science in Zoology and a Minor in Chemistry, 1983 Howard Universit
y, Washington, D.C.
PROFESSIONAL EXPERIENCE:
Senior Associate Scientist, Celera, Rockville, MD
6/03 to 7/09
Support association studies into cardiovascular and other diseases contributing
to diagnostic discoveries. Conduct experiments to establish laboratory protocols
for high-throughput DNA-resequencing projects. Communicate findings and draft c
orrespondence to scientists. Converted R&D pipeline and procedures into GLP appr
oved laboratory capable of handling clinical sequencing which includes writing a
nd executing equipment and validation method protocols. Maintained high degree o
f data quality and reproducible laboratory results, by adhering to strict record
keeping and processing protocols.
Maintained core resequencing function such as PCR, gel electrophoresis, cycle s
equencing
DNA precipitation and resuspension and manual SNP detection via
Consed
Performed QC on reagents and products and assist in new lab setup and layout
Assisted in R&D of new technologies and protocols as necessary for new projects
Analyzed sequence data and compile post-experiment data via pre-written Unix sc
ripts
Performed general maintenance, ordering, and stocking for the labs
Manually designed primers for primer walking and clone verification studies
Performed RT-PCR for isoform analysis.
Performed indel detection and deconvolution of indels,
Familiar with SQL.
Setup and incorporated GLP Guidelines and protocols for our lab
Scored 10,000 snps for the Applera SNP Project
Specialist II, R&D Management, Applied Biosystems, Rockville, MD
7/02 to 6/03

Transferred to the Template Prep Lab to supervise a team of 3-5 personnel to hel
p increase lab productivity, reproducibility and quality.
Supervised the Template Prep Lab
Worked on R&D to optimize growth conditions for specific genomic libraries
Performed excisions for plasmid libraries and cell transformations using the el
ectroporator
Analyzed, identified and scored data from the Applera SNP Project in the Unix e
nvironment
Production Associate II, Celera Genomics, Rockville, MD
10/99 to 7/02
Hired as the first supervisor of the wet sequencing and precipitation team in th
e Production Department. Played a key part in creating and establishing a high d
egree of quality and reproducibility in this department.
 Supervised wet-presequencing and precipitation labs in the Production Departmen
t, 4-9 personnel
Prepared sequence reaction mix for PCR
Maintained Quadra 384-320 (Tomtec) and MAP 8 (Titertek) for daily operations
Maintain cohesive group/team environment through communication
Bioassay Laboratory Technician, Bioqual Inc., Rockville, MD
6/99 to 9/99
Worked on a contract with the National Institutes of Health, Child Health and Hu
man
Development Branch in contraceptive drug development. Worked with various animal
models to test drug efficacy.

Formulated and administered test compounds to rats, rabbits and guinea pigs
Immunized rabbits for antibody production
Collected blood from rats, rabbits and guinea pigs
Performed sterile surgical procedures on lab animals
Performed assays and safety studies under GLP guidelines
Radioimmunoassay Technician, Bioqual Inc., Rockville, MD
6/91 to 6/99
Worked on a contract with the National Institutes of Health, Child Health and Hu
man Development Branch in contraceptive drug development
Optimized 125I and 3H radioimmunoassay of test compounds for protein and steroi
d hormones
Purified 125I radioligands via HPLC
Performed antibody titer test and ELISAs
Receptor binding assays using progestin, estrogen and androgen receptors
Trained incoming personnel
Research Biochemical/Tissue Culture Technician, Biocon Inc., Rockville, MD, 6/87
to 6/91

Worked on a contract for National Institute of Alcohol Abuse and Alcoholism to s

tudy Cystic Fibrosis
Spectrophotometry
Flourometry
Aseptic Tissue Culture
Atomic Absorption Spectroscopy
Gel Electrophoresis

CERTIFICATION:
Introduction to Unix: Principles and Practice, June 2006
Toxicology : Principles and Applications and Toxicology : Chemical Mechanism, A
merican Chemical Society, October 2004
Registered Laboratory Animal Technician, American Association for Laboratory An
imal Science, 1999
Laboratory Animal Technician (LAT) Certification, American Association of Labor
atory Animal Science, 1997
OSHA Blood borne Pathogen Training, Celera., 2005, 2006 and 2007
Fundamental Radiation Safety Training, CSI, 2006
FDA/EPA GLP Regulations, Bioqual, Inc., 1998, 1996
Fire Extinguisher Safety Training, Guardian, 2005, 2006
Publications:
Myers, E.W., Sutton, G.G., Smith, H.O., Adams, M.D., and Venter, J.C. 2002. O
n the sequencing and assembly of the human genome. Proc. Natl. Acad. Sci. USA 9
9:3713.
Mural, R.J., Adams, M.D., and Venter, J.C. 2002. A comparison of whole genome
shotgun-derived mouse chromosome 16 and the human genome. Science, 296:1661-167
1.