YU-TING HU (PH.D.

)
Department of Biological and Environmental Engineering
Cornell University
Ithaca, New York 14853-2703
Tel: (808) 282-2910 (c) Email: yh8b856e@westpost.net
Summary of Career Achievements
* Effectively improved the product yield in bacterium Acetobacter xylinum by mod
ulating the metabolic pathway through antisense RNA technology and quorum-sensin
g system.
* Profitably acquired 26 enzyme protease variants with improved activity or shif
ted optimal pH and temperature through protein engineering.
* Made meaningful contribution to the building up of a bio-pesticide company by
greatly improved the strain activity and successfully executed the scale-up of a
vermectin.
* Solved the nationwide tetracycline abnormal fermentation problem, saved millio
ns of dollars for the enterprises.
* Significantly improved the strains activity of terramycin and pravastatin thro
ugh chemical mutagenesis and adjustment of media and growth condition.
* Characterized and published a new microorganism species in the peer reviewed j
ournal.
* Creatively engineered a bacterium strain to produce 9*-hydroxy-androst-4-ene-3
, 17-dione for industrial use.
* Patented one new drug targeting diabetes from microbial resource which is in p
hase III clinical trial.
* Marketed a microbial community which deodorizes the household wastes.
* Invited as GMP consultant by several biomedical and pharmaceutical companies,
led, organized and carried out the GMP certifying work.
* Associate Professor of Graduate School, Chinese Academy of Sciences and Adjunc
t Professor of Beijing Science and Technology University. Among the different ty
pes of courses given, Microbial Technology ranked on top in the department for t
he highest evaluation from the students and the largest number of students atte
nded.

Summary of Skills
* Thorough and systematic training in microbiology and biochemistry, majored in
Environmental Microbiology for PhD, Systematic Bacteriology for Msc and Microbia
l Biochemistry for Bsc. Postdoctoral training was on protein engineering, metabo
lic engineering and proteomics.
* Solid knowledge and hands-on experience in microbial isolation, characterizati
on, physiology, metabolism and genetics of various microorganisms, such as E. co
li, yeast (Saccharomyces and Pichia), Streptomyces, Bacillus, Micromonospora, Ro
dococcus, Mycobacterium, Burkholderia, Saccharothrix, Acetobacter etc.
* Much experience in microbial metabolics and proteomics, fingerprinting metabol
ites and enzymes in the biodegradation of environmental pollutants, such as poly
cyclic aromatic hydrocarbons. Skilled in HPLC, GC, and 2-D LC-MS.
* 12 years' pharmaceutical industrial experience, including: 7 years' with new d
rug research and development, fermentation technology and high throughput screen
ing, 5 years' with in-vitro diagnostics tests for Hepatitis series, HIV, syphili
s, fertility and tumor markers.
* Skills related to molecular biology and biochemistry: PCR, RT-PCR, gene's clon
e and expression, Southern Blotting, Northern Blotting, DNA and cDNA library con
struction and screening, error-prone PCR, DNA in vitro shuffling, antisense RNA
technology, quorum-sensing, Western Blotting, SDS-PAGE, native PAGE.
* Proficiency in protein engineering including: site-directed mutagenesis, direc
ted enzyme evolution, protein purification, protein expression in E.coli and yea
st also with different cell-free systems.
* Extensive experience with different enzymes, including cellulase, protease, di
oxygenase and amylase, which involved in enzyme synergetic effect, enzyme kineti
cs, high throughput screening of enzyme variants, mutagenesis design and analysi
s, variants' characterization, enzyme gene's clone and expression.
* Drafting and editing GMP documents, including SOPs and validation projects for
pharmaceutical as well as in-vitro diagnostics reagents companies.
* 5 years' teaching experience in Graduate School, Chinese Academy of Sciences.

Professional Experience
2006- present Cornell University, Ithaca, NY, Research Associate, Post Doctoral
Associate.
Use antisense RNA technology and quorum sensing system modulating the metabolic
pathway
of Acetobacter xylinum to improve the product yield.
Work on protein engineering of cellulase and protease, including design and anal
ysis of mutants,
site-directed mutagenesis; expression and purification of enzyme variants; direc
ted
enzyme evolution through error-prone PCR and DNA shuffling; high throughput scre
ening
and enzyme assay with 96-well plate; characterization of enzyme variants.
2005- 2006 Department of Molecular Biosciences and Bioengineering, University o
f Hawaii
at Manoa, Honolulu, HI, Junior Researcher. Researched biodegradation of environ
mental
pollutants through genetic, metabolic and proteomic methods, including gene's
clone and
expression of dioxygenase, metabolic pathways of phenanthrene in different micro
organisms,
enzyme profiling in response to polycyclic aromatic hydrocarbons.
2004- 2005 Graduate School, Chinese Academy of Sciences, Beijing, China, Associa
te Professor. Constructed an industrial microorganism to accumulate 9*-hydroxy-a
ndrost-4-ene-3, 17-dione through genetic engineering method by blocking or deact
ivating the side metabolic pathways. Course teaching of Environmental Microbiolo
gy.
2001-2004 Institute of Microbiology, Chinese Academy of Sciences, Beijing, Chin
a, Ph.D.
Candidate. Studied degrading enzyme dioxygenase and metabolic pathways of phenan
threne
and pyrene in microorganism Saccharothrix sp., an oligotrophic bacterium isolat
ed from an
unpolluted lake.
1997- 2001 Graduate School, Chinese Academy of Sciences, Beijing, China, Associ
ate
Professor, Senior Engineer. Applied photosynthetic bacteria
to the waste water treatment.
 Different types of course teaching, including Microbial Tech
nology, General Microbiology,
General Biology, Molecular Biology etc.
1990 - 1997 New Drug Research and Development Center, North China Pharmaceutical
Corporation, Shijiazhuang, China, Engineer (Microbiologist). Worked on new drug
R&D,
Fermentation Technology and high throughput screening of industrial microorganis
ms.
Patent
Ivastatin--the novel *-amylase Inhibitor and its microorganism, Chinese inventio
n patent, patent number: ZL 93 1 17400.7.1999.
Honors and Awards
* Avermectin, microorganism and fermentation technology scale-up. Science and Te
chnology Advancement Prize of North China Pharmaceutical Corporation, 1996.
* Ivastatin, the screening and the identification of host microorganism. Science
and Technology Advancement Prize of North China Pharmaceutical Corporation, 199
4. Nominated for China Top Ten Youths by Chinese Food and Drug Administration, 1
995.
* Phage- like phenomenon in the fermentation of Tetracycline and the solution to
the problem, National Prize of Chinese Antibiotics Association, 1993.
Education
Ph.D. Environmental Microbiology, Chinese Academy of Sciences, 2004.
M. S. Systematic Bacteriology, Chinese Academy of Sciences, 1990.
B.S. Microbial Biochemistry, Hebei University, 1987.
Membership
Member of American Society for Microbiology
Research Papers
1. Yu-Ting Hu, Xin Gen Lei. Heterologous expression of a keratin degrading enzym
e (TfpA) in Saccharomyces cerevisiae and its rapid directed evolution. (in prepa
ration)
2. Yu-Ting Hu, Xin Gen Lei. Reductase activity of a serine protease (TfpA): sit
e-directed mutagenesis and biochemical mechanism of keratin degradation (in prep
aration)
3. Seo JS, Keum YS, Hu Y, Lee SE, Li QX. 2007. Degradation of phenanthrene by Bu
rkholderia sp. C3: initial 1,2- and 3,4-dioxygenation and meta- and ortho-cleava
ge of naphthalene-1,2-diol. Biodegradation. 18(1):123-31.
4. Seo, J. S, Y. S Keum, Y. Hu, S. E. Lee, Q. X. Li. 2006. Phenanthrene degrada
tion in Arthrobacter sp. P1-1: Initial 1,2-, 3,4- and 9,10-dioxygenation, and me
ta- and ortho-cleavages of naphthalene-1,2-diol after its formation from naphtha
lene-1,2-dicarboxylic acid and hydroxyl naphthoic acids. Chemosphere. 65(11):238
8-94.
5. Keum, Y. S, J. S Seo, Y. Hu, Q. X. Li. 2006. Degradation pathways of phenanth
rene by Sinorhizobium sp. C4. Appl. Microbiol. Biotechnol. 71(6): 935-41.
6. Yu-Ting Hu, Pei-Jin Zhou, Yu-Guang Zhou, and Shuang-Jiang Liu, "Saccharothrix
xinjiangensis sp. nov., a pyrene-degrading actinomyces from unpolluted lake", I
nternational Journal of Systematic and Evolutionary Microbiology, Vol. 54, 2091-
2094 (2004)
7. Yuting Hu, Fenghua Ren, Peijin Zhou, Min Xia, and Shuangjiang Liu, "Degradati
on of pyrene and characterization of Saccharothrix sp. PYX-6 from the oligotroph
ic Tianchi Lake in Xinjiang Uygur Autonomous Region, China", Chinese Science Bul
letin (English edition), Vol.48 (20), 2210-2215 (2003).
8. Yuting Hu, Fenghua Ren, Peijin Zhou, Min Xia, and Shuangjiang Liu, "Degradati
on of pyrene and characterization of Saccharothrix sp. PYX-6 from the oligotroph
ic Tianchi Lake in Xinjiang Uygur Autonomous Region, China", Chinese Science Bul
letin (Chinese edition), Vol.48 (20), 2210-2215 (2003).
9. Yuting Hu, Jiaxi Wang, "Research advance on interleukin-18 binding protein".
Foreign Medical Sciences, Vol. 23, 95-97 (2001).
10. Yuting Hu, "Teaching experiences on Microbial Technology", Acta Microbiology
Sinica, Vol. 1, 36-38 (2001).
11. Yuting Hu, Donggang Xu, Baikun Ma, and Jiaxi Wang, "Clone and sequence analy
sis of Chinese interleukin-18 (IL-18) binding protein", Bulletin Of Medical Rese
arch, Vol. 29, 48-50 (2000).
Major Abstracts and Presentations
1. Yuting Hu, Il-Kyu Cho, Sung-Eun Lee, Jong-Su Seo, Young-Soo Keum, and Qing X.
Li. Enzyme profiles in response to polycyclic aromatic hydrocarbons in Burkhold
eria sp. C3. 232nd ACS National Meeting. September 10-14, 2006. San Francisco, C
A.
2. Yuting Hu, Jong-Su Seo, Christiane Hennessee, Young-Soo Keum, Qing X. Li. Co
mparative studies of aromatic dioxygenases in various polycyclic aromatic hydroc
arbon -degrading bacterial taxa. Pacifichem International Congress. December 1
5 - 20, 2005. Honolulu, Hawaii.
3. Yuting Hu, Chengying Jiang, Shen Xihui, Wang Baojun, Fenghua Ren, Zhipei Liu,
Shuangjiang Liu. An oligatrophic bacterium that can degrade polycyclic aromati
c hydrocarbons. Thesis collections of 50 anniversaries for China Microbiologica
l Society. November 19-22, 2002. Beijing, China.
4. Yuting Hu, "Research on DNA vaccine", Collections of Thesis on 21st Agricultu
ral Biotechnology. 29-30 (1999).