FRANK W. SUMMERFIELD, Ph. D.

St. Paul, MN 55108

650-533-4231
fs1f1262@westpost.net
OBJECTIVE
Research and Development in Bioscience
SUMMARY OF SKILLS
* HPLC, FPLC, PIC, IEX, SEC, PAGE, IEF, ultrafiltration
* XRF, ICP, FT NMR
* Bioassay development, biomedical and bioanalytical chemistry
* Protein purification, chemistry, characterization, and activity analysis
* Synthetic organic, inorganic, and analytical chemistry
* Team building and management, project coordination, 510-ks
* Scientific and technical writing, bioinformatics, database and patent searc
hing
EDUCATION
* Postdoctoral Fellowship, University of California, Berkeley, CA, Physiology
* Ph. D. University of California, Davis, CA, Biochemistry / Biophysics
* M. Sc. University of Hawaii, Honolulu, HI, Biochemistry / Biophysics
* B. Sc. Rider College, Lawrenceville, NJ, Chemistry
PROFESSIONAL EXPERIENCE
BIOSCIENCE RESEARCH AND DEVELOPMENT
* University of Minnesota, St. Paul, MN, Research Scientist, 2009-2010
Utilized IEF, mass spectrometry, and other methods to isolate and obtain se
quence information on fungal proteins potentially capable of degrading lignins;
identified families of enzymes with possible similar lignin-degrading activity t
hrough NCBI and other databases; correlated sequence information on these enzyme
s via BLAST; tested some enzymes on model substrates; obtained information on po
ssible mechanisms of these enzymes via TLC, difference spectroscopy, mass spectr
ometry, and other means.
* Chronomed, San Carlos, CA, Scientific Consultant, 2007-2009
Developed new synthetic techniques to extend the life of affinity resins to
detect diabetes by a point-of-care format.
* Cytobiologics, Half Moon Bay, CA, Research Associate, 2005
Cultured mouse cells to make monoclonal antibodies; purified them by affini
ty chromatography using protein A or specific antigens; made a purer product.
* Geronova, Fairfax, CA, Scientific Consultant, 2005
Developed new and more sensitive methods to measure lipoic acid, a naturally
occurring compound useful in treating diabetes, in product samples and in plasm
a by a variety of HPLC and fluorescence methods measured kinetics and dynamics o
f metabolism of lipoic acid in vivo.
* Chronomed, San Carlos, CA, Scientific Consultant, 2002-2004
Developed new procedures to make affinity resins to detect diabetes
by a point-of-care instrument or by HPLC.
* University of Minnesota, St. Paul, MN, Research Scientist, 2002
Studied the biodegradation of lignins by white rot fungus; isolated and
 investigated proteins that may degrade lignins to liberate cellulose for
conversion to biofuels; obtained sequence information on these proteins
by mass spectrometry to design a PCR probe.
* RxQuest, San Mateo, CA, Scientist, Co-founder, and CSO, 1996-2001
Conceived and designed the chemistry and complete plans for a portable,
self-contained biomedical analyzer to simultaneously measure multiple
proteins, metabolites, or other molecules in blood, plasma, other biological
fluids, or air; used HTP methods in optimizing assays.
OTHER CAREER HIGHLIGHTS
* Developed methods to separate fetal erythrocytes from maternal circulation
for cytogenetic analysis for early determination of genetic abnormalities; made
a better product.
* Investigated ferulic acid, a natural alternative to anabolic steroids; devi
sed new synthetic methods; demonstrated safety and effectiveness of the product.
* Fabricated antibody-dependent, permeablized glass microelectrodes to develo
p an electroimmunoassay for antigens, metabolites, and other compounds; correlat
ed results with those of ELISA and other methods.
* Managed a team to develop new ways to synthesize purer and less expensive o
rganogermanium nutritional supplements; investigated the use of biotechnology to
produce these compounds; used XRF, FT NMR, and other methods to ascertain purit
y; instituted QC, GLP, and GMP; increased total sales 120%.
SCIENTIFIC AND POPULAR WRITING
* FitnessHeaven.com, Signal Hill, CA, Feature Writer
Researched and wrote articles of popular interest on scientific topics, such
as diabetes, exercise, stress, flavonoids, other antioxidants, and the optimiz
ation of buccal and trans-dermal absorption of pharmaceuticals, vitamins, and ot
her organic materials.
* Life Extension Foundation, Hollywood, FL, Scientific Writer
Researched and wrote articles of popular interest in ant-aging topics, such
as antioxidamts, free radicals, protective nutraceuticals, and various regimens
and lifestyle changes to maximize health and lifespan. (The Anti-Aging News bec
ame Life Extension Report and then Life Extension Magazine.)
AFFILIATIONS
* American Academy of Anti-Aging Medicine (A4M)
* American Aging Association (AGE)
* Bay Area Bioscience Forum (Formerly NCPDG)
* International Angel Investors Association (IAI)
* Northern California Venture Capital Association (NCVCA)

CONTINUING EDUCATION AND TRAINING

* American Academy of Anti-Aging Medicine (A4M) conference, Las Vegas, NV, 20
08.
* UABBA anti-aging conference, Los Angeles, CA, 2008.
* FASEB conference, San Francisco, CA, 2006.
* SENS II anti-aging conference, Cambridge, UK, 2005.
* American Aging Association (AGE) conference, Oakland, CA, 2005.
* Training in modern biotechnology, Cal State, San Jose, CA, 2000.
PUBLICATIONS
Summerfield, F.W.
Ellagic Acid: Cancer Cure under Our Nose (2001) FitnessHeaven.com, 1/01.
Flavonoids: the Alternative Antioxidants (2000) FitnessHeaven.com, 9/00.
Advances in Diabetes Treatment (1996) Life Ext. Mag. 2, 20-24.
Silymarin: Your Liver's Best Friend (1991) Life Ext. Rep. 11, 9-12.

Finding the Genes that Trigger Aging: A Proposal (1989) Life Ext. Rep. 9,
89-94.
Model Systems for the Study of Aging (1989) Life Ext. Rep. 9, 81-85.
Germanium and Health (1988) Life Ext. Rep. 8, 89-93.
Aging and Mitochondrial DNA (1986) Life Ext. Rep. 6, 80-84.
Effects of Stress on Health and Aging (1986) Life Ext. Rep. 6, 53-56.
Cancer from the Environment: What You Can Do About It (1985) Anti-Aging
News 5, 89-93.
Exercise, Health, and Aging (1985) Anti-Aging News 6, 43-46.
Free Radicals, DNA Damage, and Aging (1985) Anti-Aging News 5, 37-41.
Summerfield, F.W. & Tappel, A.L.
Effects of Polyunsaturated Fats and Vitamin E on Aging and Peroxidative
Damage to DNA (1984) Arch. Bioch. Bioph. 233, 408-416.
Peroxidative Damage to DNA Caused by High Dietary Polyunsaturated Fats
and Vitamin E Deficiency (1984) Fed. Proc. 42, 2089.
Peroxidative Damage to DNA by Polyunsaturated Fats and Protection by
Vitamin E (1984) Age 6, 144.
Vitamin E Protects against Methyl Ethyl Ketone Peroxide-Induced
Peroxidative Damage to Rat Brain DNA (1984) Mut. Res. 126, 113-120.
Crosslinking of DNA by Malondialdehyde Produced from 1,3-Propanediol in
Vivo (1984) Chem-Biol. Inter. 50, 87-96.
Detection and Measurement by HPLC of Malondialdehyde Crosslinks in DNA
(1984) Anal. Bioch. 143, 365-371.
DNA Crosslinks Made by Malondialdehyde: Structure and Environment Determined
by Fluorescence Quenching (1983) Bioch. Bioph. Acta 740, 185-189.
REFERENCES: Available upon request.