FE 409 9F2 E35 563 20DD90 6A 807 022 8B4

E 5 6 8 0 8
E4 409F F2E 355 632 20D 906 A80 070 228 B49 9C2
09 2E 355 63 0D 906 A8 70 228 B4 C2 FE
09 F2E 355 63 20D 906 A8 070 228 B4 9C2 FE 409
F 2
9F 2E3 3556 632 0D9 906AA80 0702 228B B49 9C2F FE4 409F F2E
2 E 5 3 0 3
F2 35 563 20 D90 06A 807 7022 28B 49C C2FE E40 09F2 2E3 556
E3 56 20 D9 6A 80 02 8B 49 2F 4 9F E3 556 32
E3 556 320 D9 06A 80 702 28B 49 C2F E4 09F 2E3 556 32 0D9
5 0 7 2 C 0 2 0
35 563 320DD90 6A8 807 022 8B4 49C 2FE E409 9F2 E35 5563 320DD90 06A
56 20 9 6A 07 02 8B 9C 2F 40 F2 E3 56 20 9 6A 80
55 32 D 06 80 0 28 49 2 E4 9F E 55 32 D 06 8 70
63 0 90 A 7 22 B C FE 0 2 3 6 0 90 A 07 22

64789
63 20DD90 6A8 8070 022 8B4 49C 2FE 409 9F2E E35 5563 320DD90 6A8 807 022 8B4
2 6 8 2 6 0 8
32 0D9 906 A80 0702 228 B49 9C2 FE4 409F F2E 355 5632 20D 906 A80 070 228 B49 9C2
0D 06 A8 70 28 B4 C2 FE 09 2E 35 63 0D 90 A8 70 22 B4 C FE
90 A8 07 22 B4 9C FE 409 F2 35 563 20D 90 6A 07 22 8B 9C 2FE 40
6A 07 02 8B 9C 2F 40 F E3 56 20 9 6A 80 02 8B 49 2F 4 9F
80 02 28B 49 2F E4 9F 2E3 556 32 D9 06A 80 702 28B 49 C2F E4 09F 2E

confluency.
Time 2:30 hrs.

70 28 49 C2 E4 09 2E 55 32 0D 06 8 70 28 4 C2 E 09 2E 35
22 B4 C FE 09 F2 35 63 0D 90 A 07 22 B 9C FE 40 F2 35 56

6. Define–Callus.
8B 9C 2F 40 F E3 56 20 9 6A 80 02 8B 49 2F 4 9F E3 56 32
49 2F E4 9F 2E3 55 32 D9 06 80 702 28 49 C2 E4 09F 2E 55 32 0D

14. Fill in the blank:

13. Fill in the blank:
C2 E 09 2E 55 63 0D 06 A8 70 28 B4 C2 FE 09 2E 35 63 0D 90
FE 409 F2 35 63 20D 90 A 07 22 B4 9C FE 40 F2 35 563 20 90 6A
40 F2 E35 56 20 90 6A 807 022 8B 9C 2F 40 9F2 E3 56 20 D9 6A 807
9F E3 56 32 D9 6A 80 02 8B 49 2F E4 9F E 55 32 D 06 80 02

culture laboratory.
adult plant is retained.
2E 55 32 0D 06 8 70 28 4 C2 E 09 2E 355 63 0D 906 A8 70 28

8. Define:Feeding of cells.
35 63 0D 90 A8 07 22 B4 9C FE 409 F2 35 63 20D 90 A 07 22 B4
56 20 9 6A 07 02 8B 9C 2F 40 F2 E3 56 20 9 6A 80 02 8B 9C
Q.1 Do as directed (ANY 15)

32 D9 06 80 02 28 49 2 E4 9F E 55 32 D 06 80 70 28 49 2
0D 06 A8 70 28 B4 C2 FE 09 2E 35 63 0D 90 A8 70 22 B4 C FE

16. Explain the term plagiarism.

1. All questions are compulsory.

90 A8 07 22 B4 9C FE 409 F2 35 563 20D 90 6A 07 22 8B 9C 2FE 40

2. Figures to right indicate marks.

6A 07 02 8B 9C 2F 40 F E3 56 20 9 6A 80 02 8B 49 2F 4 9F
80 02 28B 49 2F E4 9F 2E3 556 32 D9 06A 80 702 28B 49 C2F E4 09F 2E
70 28 49 C2 E4 09 2E 55 32 0D 06 8 70 28 4 C2 E 09 2E 35
22 B4 C FE 09 F2 35 63 0D 90 A 07 22 B 9C FE 40 F2 35 56
8B 9C 2F 40 F E3 56 20 9 6A 80 02 8B 49 2F 4 9F E3 56 32
49 2F E4 9F 2E3 55 32 D9 06 80 702 28 49 C2 E4 09F 2E 55 32 0D

9. Define: Passage in animal cell culture.

C2 E 09 2E 55 63 0D 06 A8 70 28 B4 C2 FE 09 2E 35 63 0D 90
FE 409 F2 35 63 20D 90 A 07 22 B4 9C FE 40 F2 35 563 20 90 6A
40 F2 E35 56 20 90 6A 807 022 8B 9C 2F 40 9F2 E3 56 20 D9 6A 807
9F E3 56 32 D9 6A 80 02 8B 49 2F E4 9F E 55 32 D 06 80 02
2E 55 32 0D 06 8 70 28 4 C2 E 09 2E 355 63 0D 906 A8 70 28

20. Communication is _________ way process.

hormone in plant tissue culture medium.

35 63 0D 90 A8 07 22 B4 9C FE 409 F2 35 63 20D 90 A 07 22 B4

OR
56 20 9 6A 07 02 8B 9C 2F 40 F2 E3 56 20 9 6A 80 02 8B 9C
32 D9 06 80 02 28 49 2 E4 9F E 55 32 D 06 80 70 28 49 2

Tissue culture was first developed by __________.

OR
0D 06 A8 70 28 B4 C2 FE 09 2E 35 63 0D 90 A8 70 22 B4 C FE
3. Give one example of plant tissue culture medium.

90 A8 07 22 B4 9C FE 409 F2 35 563 20D 90 6A 07 22 8B 9C 2FE 40

18. Enlist any two examples of verbal communication.

6A 07 02 8B 9C 2F 40 F E3 56 20 9 6A 80 02 8B 49 2F 4 9F

Page 1 of 2
80 02 28B 49 2F E4 9F 2E3 556 32 D9 06A 80 702 28B 49 C2F E4 09F 2E
70 28 49 C2 E4 09 2E 55 32 0D 06 8 70 28 4 C2 E 09 2E 35
22 B4 C FE 09 F2 35 63 0D 90 A 07 22 B 9C FE 40 F2 35 56
4. Give the significance of antibiotic used in PTC medium.

8B 9C 2F 40 F E3 56 20 9 6A 80 02 8B 49 2F 4 9F E3 56 32
12. Give one example of intracellular activity in animal cell.
49 2F E4 9F 2E3 55 32 D9 06 80 702 28 49 C2 E4 09F 2E 55 32 0D
3. Draw neat labeled diagrams/structures wherever necessary.

Q.2 B) Discuss the importance of totipotency in plant science.

C2 E 09 2E 55 63 0D 06 A8 70 28 B4 C2 FE 09 2E 35 63 0D 90
FE 409 F2 35 63 20D 90 A 07 22 B4 9C FE 40 F2 35 563 20 90 6A
7. What does MLMH stands for in plant tissue culture medium?

FE409F2E3556320D906A8070228B49C2
40 F2 E35 56 20 90 6A 807 022 8B 9C 2F 40 9F2 E3 56 20 D9 6A 807
9F E3 56 32 D9 6A 80 02 8B 49 2F E4 9F E 55 32 D 06 80 02
10. State true or false: Tumor cells give rise to continuous cell lines

2E 55 32 0D 06 8 70 28 4 C2 E 09 2E 355 63 0D 906 A8 70 28

Q.3 B) Give methods of sterilization of animal tissue culture media.

___________ tissue was first used as first source for cell culture.
35 63 0D 90 A8 07 22 B4 9C FE 409 F2 35 63 20D 90 A 07 22 B4
56 20 9 6A 07 02 8B 9C 2F 40 F2 E3 56 20 9 6A 80 02 8B 9C
32 D9 06 80 02 28 49 2 E4 9F E 55 32 D 06 80 70 28 49 2

Q.2 D) Discuss the protocol for callus culture of the carrot root in detail.
0D 06 A8 70 28 B4 C2 FE 09 2E 35 63 0D 90 A8 70 22 B4 C FE
90 A8 07 22 B4 9C FE 409 F2 35 563 20D 90 6A 07 22 8B 9C 2FE 40
6A 07 02 8B 9C 2F 40 F E3 56 20 9 6A 80 02 8B 49 2F 4 9F
80 02 28B 49 2F E4 9F 2E3 556 32 D9 06A 80 702 28B 49 C2F E4 09F 2E

Q.3 A) Define primary culture? Explain how to obtain a primary cell culture.
70 28 49 C2 E4 09 2E 55 32 0D 06 8 70 28 4 C2 E 09 2E 35
22 B4 C FE 09 F2 35 63 0D 90 A 07 22 B 9C FE 40 F2 35 56
8B 9C 2F 40 F E3 56 20 9 6A 80 02 8B 49 2F 4 9F E3 56 32
49 2F E4 9F 2E3 55 32 D9 06 80 702 28 49 C2 E4 09F 2E 55 32 0D
C2 E 09 2E 55 63 0D 06 A8 70 28 B4 C2 FE 09 2E 35 63 0D 90
FE 409 F2 35 63 20D 90 A 07 22 B4 9C FE 40 F2 35 563 20 90 6
40 F2 E35 56 20 90 6A 807 022 8B 9C 2F 40 9F2 E3 56 20 D9 6A
9F E3 56 32 D9 6A 80 02 8B 49 2F E4 9F E 55 32 D 06
17. Language is one of the physiological barrier of communication. (State true or false)
15. Power point presentation is used for writing the research report.(State True or False)

2E 55 32 0D 06 8 70 28 4 C2 E 09 2E 355 63 0D 906 A8
35 63 0D 90 A8 07 22 B4 9C FE 409 F2 35 63 20D 90 A
56 20 9 6A 07 02 8B 9C 2F 40 F2 E3 56 20 9 6A 80
19. ____________ is a type of communication that employs gestures and body language.
1. Name one method for the isolation of single cell from plant organ in plant tissue culture.

32 D9 06 80 02 28 49 2 E4 9F E 55 32 D 06 80 7
0D 06 A8 70 28 B4 C2 FE 09 2E 35 63 0D 90 A8 70

(07)
(08)
(07)
(08)

(07)
(08)
(15)

90 A8 07 22 B4 9C FE 409 F2 35 563 20D 90 6A 07

6A 07 02 8B 9C 2F 40 F E3 56 20 9 6A 80 02
Marks:75

80 02 28B 49 2F E4 9F 2E3 556 32 D9 06A 80 702 2

70 28 49 C2 E4 09 2E 55 32 0D 06 8 70 28
5. Name the German scientist who developed the concept of invitro cell cultures of the plant tissue.
2. _________is the cell characteristics in which the potential for forming all the cell types in an

11. State true or false: Cells from normal tissues show density dependency and contact inhibition at

Q.2C)What are macronutrients? Discuss the role of macronutrients and any two plant growth
Q.2 A) Explain in detail the organization and role of culture room and transfer area in plant tissue
22 B4 C FE 09 F2 35 63 0D 90 A 07 22
8B 9C 2F 40 F E3 56 20 9 6A 80 02 8B
Paper / Subject Code: 77404 / Biotechnology-I: Tissue Culture & Scientific Writing & Communication.

49 2F E4 9F 2E3 55 32 D9 06 80 702 28
C2 E 09 2E 55 63 0D 06 A8 70 28 B4
FE 409 F2 35 63 20D 90 A 07 22 B4
40 F2 E35 56 20 90 6A 807 022 8B 9C
9F E3 56 32 D9 6A 80 02 8B 49
2E 55 32 0D 06 8 70 28 4 C2
3 6 0 9 A 0 2 B 9
FE 409 9F2 E35 563 20DD90 6A 807 022 8B4
E 5 6 8 0 8
E4 409F F2E 355 632 20D 906 A80 070 228 B49 9C2
09 2E 355 63 0D 906 A8 70 228 B4 C2 FE
09 F2E 355 63 20D 906 A8 070 228 B4 9C2 FE 409
F 2
9F 2E3 3556 632 0D9 906AA80 0702 228B B49 9C2F FE4 409F F2E
2 E 5 3 0 3
F2 35 563 20 D90 06A 807 7022 28B 49C C2FE E40 09F2 2E3 556
E3 56 20 D9 6A 80 02 8B 49 2F 4 9F E3 556 32
E3 556 320 D9 06A 80 702 28B 49 C2F E4 09F 2E3 556 32 0D9
5 0 7 2 C 0 2 0
35 563 320DD90 6A8 807 022 8B4 49C 2FE E409 9F2 E35 5563 320DD90 06A
56 20 9 6A 07 02 8B 9C 2F 40 F2 E3 56 20 9 6A 80
55 32 D 06 80 0 28 49 2 E4 9F E 55 32 D 06 8 70

5.
4.
3.
2.
1.
63 0 90 A 7 22 B C FE 0 2 3 6 0 90 A 07 22

64789
63 20DD90 6A8 8070 022 8B4 49C 2FE 409 9F2E E35 5563 320DD90 6A8 807 022 8B4
2 6 8 2 6 0 8
32 0D9 906 A80 0702 228 B49 9C2 FE4 409F F2E 355 5632 20D 906 A80 070 228 B49 9C2
0D 06 A8 70 28 B4 C2 FE 09 2E 35 63 0D 90 A8 70 22 B4 C FE
90 A8 07 22 B4 9C FE 409 F2 35 563 20D 90 6A 07 22 8B 9C 2FE 40
6A 07 02 8B 9C 2F 40 F E3 56 20 9 6A 80 02 8B 49 2F 4 9F
80 02 28B 49 2F E4 9F 2E3 556 32 D9 06A 80 702 28B 49 C2F E4 09F 2E
70 28 49 C2 E4 09 2E 55 32 0D 06 8 70 28 4 C2 E 09 2E 35
culturing.

22 B4 C FE 09 F2 35 63 0D 90 A 07 22 B 9C FE 40 F2 35 56
8B 9C 2F 40 F E3 56 20 9 6A 80 02 8B 49 2F 4 9F E3 56 32
49 2F E4 9F 2E3 55 32 D9 06 80 702 28 49 C2 E4 09F 2E 55 32 0D
C2 E 09 2E 55 63 0D 06 A8 70 28 B4 C2 FE 09 2E 35 63 0D 90

Growth factors.
FE 409 F2 35 63 20D 90 A 07 22 B4 9C FE 40 F2 35 563 20 90 6A
40 F2 E35 56 20 90 6A 807 022 8B 9C 2F 40 9F2 E3 56 20 D9 6A 807
9F E3 56 32 D9 6A 80 02 8B 49 2F E4 9F E 55 32 D 06 80 02
2E 55 32 0D 06 8 70 28 4 C2 E 09 2E 355 63 0D 906 A8 70 28

Inverted microscope.
35 63 0D 90 A8 07 22 B4 9C FE 409 F2 35 63 20D 90 A 07 22 B4
56 20 9 6A 07 02 8B 9C 2F 40 F2 E3 56 20 9 6A 80 02 8B 9C
32 D9 06 80 02 28 49 2 E4 9F E 55 32 D 06 80 70 28 49 2
0D 06 A8 70 28 B4 C2 FE 09 2E 35 63 0D 90 A8 70 22 B4 C FE

ii.What is the goal of the study?

90 A8 07 22 B4 9C FE 409 F2 35 563 20D 90 6A 07 22 8B 9C 2FE 40
6A 07 02 8B 9C 2F 40 F E3 56 20 9 6A 80 02 8B 49 2F 4 9F

Importance of callus culture.

80 02 28B 49 2F E4 9F 2E3 556 32 D9 06A 80 702 28B 49 C2F E4 09F 2E
70 28 49 C2 E4 09 2E 55 32 0D 06 8 70 28 4 C2 E 09 2E 35
22 B4 C FE 09 F2 35 63 0D 90 A 07 22 B 9C FE 40 F2 35 56

Q.5 Write short notes on: (Any three)

8B 9C 2F 40 F E3 56 20 9 6A 80 02 8B 49 2F 4 9F E3 56 32

Use of rotary shakers in PTC lab.

49 2F E4 9F 2E3 55 32 D9 06 80 702 28 49 C2 E4 09F 2E 55 32 0D
C2 E 09 2E 55 63 0D 06 A8 70 28 B4 C2 FE 09 2E 35 63 0D 90
FE 409 F2 35 63 20D 90 A 07 22 B4 9C FE 40 F2 35 563 20 90 6A
40 F2 E35 56 20 90 6A 807 022 8B 9C 2F 40 9F2 E3 56 20 D9 6A 807

i.Suggest a suitable title to the research work.

9F E3 56 32 D9 6A 80 02 8B 49 2F E4 9F E 55 32 D 06 80 02

Importance of effective communication.

2E 55 32 0D 06 8 70 28 4 C2 E 09 2E 355 63 0D 906 A8 70 28
35 63 0D 90 A8 07 22 B4 9C FE 409 F2 35 63 20D 90 A 07 22 B4
OR

56 20 9 6A 07 02 8B 9C 2F 40 F2 E3 56 20 9 6A 80 02 8B 9C
32 D9 06 80 02 28 49 2 E4 9F E 55 32 D 06 80 70 28 49 2
0D 06 A8 70 28 B4 C2 FE 09 2E 35 63 0D 90 A8 70 22 B4 C FE
90 A8 07 22 B4 9C FE 409 F2 35 563 20D 90 6A 07 22 8B 9C 2FE 40
Q.4 A) Explain the process of communication in brief.

6A 07 02 8B 9C 2F 40 F E3 56 20 9 6A 80 02 8B 49 2F 4 9F

Page 2 of 2
*********
80 02 28B 49 2F E4 9F 2E3 556 32 D9 06A 80 702 28B 49 C2F E4 09F 2E
70 28 49 C2 E4 09 2E 55 32 0D 06 8 70 28 4 C2 E 09 2E 35
22 B4 C FE 09 F2 35 63 0D 90 A 07 22 B 9C FE 40 F2 35 56
8B 9C 2F 40 F E3 56 20 9 6A 80 02 8B 49 2F 4 9F E3 56 32
49 2F E4 9F 2E3 55 32 D9 06 80 702 28 49 C2 E4 09F 2E 55 32 0D
C2 E 09 2E 55 63 0D 06 A8 70 28 B4 C2 FE 09 2E 35 63 0D 90
FE 409 F2 35 63 20D 90 A 07 22 B4 9C FE 40 F2 35 563 20 90 6A

FE409F2E3556320D906A8070228B49C2
40 F2 E35 56 20 90 6A 807 022 8B 9C 2F 40 9F2 E3 56 20 D9 6A 807
9F E3 56 32 D9 6A 80 02 8B 49 2F E4 9F E 55 32 D 06 80 02

iv.Give four key words for the research work based on the abstract.
2E 55 32 0D 06 8 70 28 4 C2 E 09 2E 355 63 0D 906 A8 70 28

iii.Which among the two plant exhibited better antioxidant activity?

35 63 0D 90 A8 07 22 B4 9C FE 409 F2 35 63 20D 90 A 07 22 B4
Q.3 C) Write a note on any eight applications of animal tissue culture.

56 20 9 6A 07 02 8B 9C 2F 40 F2 E3 56 20 9 6A 80 02 8B 9C
32 D9 06 80 02 28 49 2 E4 9F E 55 32 D 06 80 70 28 49 2
Q.4 C) Read the following abstract and answer the following questions.

0D 06 A8 70 28 B4 C2 FE 09 2E 35 63 0D 90 A8 70 22 B4 C FE
90 A8 07 22 B4 9C FE 409 F2 35 563 20D 90 6A 07 22 8B 9C 2FE 40
6A 07 02 8B 9C 2F 40 F E3 56 20 9 6A 80 02 8B 49 2F 4 9F
80 02 28B 49 2F E4 9F 2E3 556 32 D9 06A 80 702 28B 49 C2F E4 09F 2E
70 28 49 C2 E4 09 2E 55 32 0D 06 8 70 28 4 C2 E 09 2E 35
22 B4 C FE 09 F2 35 63 0D 90 A 07 22 B 9C FE 40 F2 35 56
8B 9C 2F 40 F E3 56 20 9 6A 80 02 8B 49 2F 4 9F E3 56 32
49 2F E4 9F 2E3 55 32 D9 06 80 702 28 49 C2 E4 09F 2E 55 32 0D
C2 E 09 2E 55 63 0D 06 A8 70 28 B4 C2 FE 09 2E 35 63 0D 90
Q.4 B) Enlist the components of research paper and elaborate any two components.

FE 409 F2 35 63 20D 90 A 07 22 B4 9C FE 40 F2 35 563 20 90 6

40 F2 E35 56 20 90 6A 807 022 8B 9C 2F 40 9F2 E3 56 20 D9 6A
9F E3 56 32 D9 6A 80 02 8B 49 2F E4 9F E 55 32 D 06
2E 55 32 0D 06 8 70 28 4 C2 E 09 2E 355 63 0D 906 A8
35 63 0D 90 A8 07 22 B4 9C FE 409 F2 35 63 20D 90 A
as compared to Azadirachta indica, which was investigated by the Agar diffusion method.

56 20 9 6A 07 02 8B 9C 2F 40 F2 E3 56 20 9 6A 80
32 D9 06 80 02 28 49 2 E4 9F E 55 32 D 06 80 7

Q.4 D) Explain the important differentiating points of verbal and non-verbal communication.
0D 06 A8 70 28 B4 C2 FE 09 2E 35 63 0D 90 A8 70

(07)

(15)
(08)
(07)
(08)
(07)
(08)

90 A8 07 22 B4 9C FE 409 F2 35 563 20D 90 6A 07

6A 07 02 8B 9C 2F 40 F E3 56 20 9 6A 80 02
80 02 28B 49 2F E4 9F 2E3 556 32 D9 06A 80 702 2
Q.3 D) Draw a neat labeled diagram of carbon dioxide incubator and explain its use in animal cell

70 28 49 C2 E4 09 2E 55 32 0D 06 8 70 28
Abstract: A wide range of medicinal and aromatic plants (MAPs) have been explored for their

naturally in different plant parts during the process of secondary metabolism. The goals of the

screening of the twoplants showed presence of alkaloids, carbohydrates, glycosides, phenolic

negundo, which was investigated by (DPPH) radical scavenging assay. Regarding the antimicrobial
study were to determine and compare the phytochemical screening, antioxidant and antimicrobial

andtannin compounds, flavonoids, saponins, lipids and terpenoids. About the antioxidant potential,
activities of the essential oils of Azadirachta indica and Vitex negundo. The phytochemical

Azadirachta indica showed a higher ability to scavenge free radicals as compared to Vitex

activity, Vitex negundo showed a better ability to inhibit the growth of pathogenic micro-organisms
essential oils in the past few decades. Essential oils are complex volatile compounds, synthesized

22 B4 C FE 09 F2 35 63 0D 90 A 07 22
8B 9C 2F 40 F E3 56 20 9 6A 80 02 8B
Paper / Subject Code: 77404 / Biotechnology-I: Tissue Culture & Scientific Writing & Communication.

49 2F E4 9F 2E3 55 32 D9 06 80 702 28
C2 E 09 2E 55 63 0D 06 A8 70 28 B4
FE 409 F2 35 63 20D 90 A 07 22 B4
40 F2 E35 56 20 90 6A 807 022 8B 9C
9F E3 56 32 D9 6A 80 02 8B 49
2E 55 32 0D 06 8 70 28 4 C2
3 6 0 9 A 0 2 B 9