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Dermal fillers
Sodium hyaluronate
gel viscoelastic properties, thereby making Novozymes crosslinking technology versatile and adaptable to customers specific requirements. 5 6 Figure 1 presents the storage (G) and loss (G) moduli of a crosslinked HA hydrogel formulation as a function of the frequency of deformation. The 5 G values of formulations characterized by different ratios of crosslinked 4 HA hydrogel to HA hydrogel solution show that the elastic properties of 3 these formulations can be fine tuned (Fig. 2). Finally, the average force required to extrude different hydrogel formulations through a 30G needle 2 was below 15 N which shows that these could easily be injected in vivo 1 (Fig. 3). The well-defined rheological properties and excellent injectability of 0 Novozymes crosslinked HA formulations can offer maximum0 convenience to practitioners and comfort to patients for enhanced precision and efficacy. 1.0
Extrusion (N)
Extrusi
15
6 5
Extrusion force (N)
Extrusion (N)
10
4 3 2 1 0
2.0Formulation 1
Formulation 1
1.0
Formulation 2
2.0
Formulation 3
3.0
3.0 Formulation 2
1000
HA concentration (% w/v) Fig. 3. Average extrusion force Formulation inject 2 Formulation 3 HA concentration (% w/v) needed1 to Formulation different Novozymes crosslinked HA hydrogel formulations, differing by the ratio of crosslinked HA hydrogel to HA hydrogel solution, through a 30G needle (according to texture analysis). Formulation G Formulation 2 Formulation11 30Formulation 2 22 G 27 G Formulation 3 30 G 22 G 27 G
100
Benefits of Novozymes crosslinked HA technology Proven and versatile technology affording gels with customized viscoelastic properties No detectable residual crosslinking agent Ability to withstand autoclaving and extrusion without significant alteration of rheological properties Optimal injection mechanics for increased practitioner convenience and patient comfort Well tolerated, safe, and biocompatible
10
0.01
0.1
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Frequency (Hz)
Fig. 1. Storage (G) and loss (G) moduli as a function of the frequency of deformation of a typical Novozymes crosslinked HA hydrogel formulation (according to rheological analysis). Storage modulus Loss modulus
1000
100
References
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1
Sturm et al. A systematic review of dermal fillers for age-related lines and wrinkles. ANZ
Beasley et al. Hyaluronic acid fillers: a comprehensive review. Facial Plastic Surgery (2009)
Novozymes A/S No. 2011-15533-01
1 0.01 0.1 1 10
25, 8694.
3
Frequency (Hz)
Ghersetich and Lotti. Hyaluronic acid in cutaneous intrinsic aging. International Journal of
and their correlation with water content in human skin. Journal of Dermatological Science (2011) doi:10.1016/j.dermsci.2011.02.007.
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