PARALAB PRIVATE LIMITED Goregaon (e) MUMBAI - 400063

STANDARD OPERATING PROCEDURE MICROBIOLOGY DEPARTMENT TITLE : OPERATION OF MICROSCOPE 1.0 OBJECTIVE : To lay down a procedure for the operation of Microscope 2.0 1.0

REF. NO. SUPERSEDES ISSUE NO.

SOP/MIC/025 -1.0

REV. NO.

0.0

EFFECTIVE DATE REVIEW DATE PAGE NO. ID NO:

13.12.2010 13.11.2013 1 of 1 PPL/INST/MIC/025

2.0 3.0

SCOPE : OBJECTIVE : This procedure procedure forfor operation of Microscope To lay down a is applicable the operation of Zone Reader ID No : PPL/INST/MIC/025 Make : Labo Location : Microbiology Department SCOPE : RESPONSIBILITY : This procedure is applicable for operation of Zone Reader HOD shall be overall responsible for Ketan LocationMicroscope ID No : PPL/INST/ZR/026 Make : the operation of : Microbiology Department

4.0 PROCEDURE : 3.0 RESPONSIBILITY : 4.1 OPERATION overall responsible for the operation of Zone Reader HOD shall be 4.1.1 Keep the microscope in position. 4.1.2 Adjust the low power objecitve, by rotating the revolving nose piece in postion. 4.0 PROCEDURE : 4.1.3 Adjust the focus of light with the help of mirror & condenser. 4.1 OPERATION 4.1.4 Ensure that the instrument (high power or from dust. Now place the objective is clean & free oil immersion whichever is requried ) in position by y moving the mains. y Switch on the resolving nose piece. 4.1.5 There isthe slide on the stage, lower the side of thewhile observing through the eye piece, till the Place ON/OFF switch on the front objective instrument. Put the switch to ON. y field appears clearly (if the oil immersion objective is in of the zoneone drop of be ON. oil y The indicator lamp will be ON, & the main light source use, place reader will immersion on the slide before placing it on the stage & then lower the O.I. objective just to touch the oil y Set the vernier scale to Zero-Zero coincidence. drop. y Place the microbiological Assay plate & measure accurately the zone of inhibition or zone of 4.1.6 Make the field clearer with the help of fine adjustment & then observe the slide. exhibition. 4.1.7 Slide should be move in left ,right , upper & the lower direction to observe the complete y The scale reading givesof slide stage screw & rotating scale, & 1/5mm on the fixed vernier smear area with help nearest 0.2mm on the coarse adjustment. scale. completion of observation, remove the slide , clean the objective lens & condenser with 4.1.8 After y Remove the plate after the readings are obtained. cotton soaked in xylene. Lower down the condenser & place low power obejctive in position. y 4.1.9 Put off the light. oil to harden on the lens. Do not allow the y Clean the instrument with a disinfectant by swabbing. y Switch OFF the mains & keep the instrument under cover when not in use. 5.0 ABBREVIATION  SOP : Standard Operating Procedure 5.0  O.I. ABBREVIATION : Oil Immersion  SOP : Standard Operating Procedure  Mm REFERENCE : Millimeter 6.0 Operating Manual 6.0 REFERENCE Operating Manual

PARALAB PRIVATE LIMITED Goregaon (e) MUMBAI - 400063

STANDARD OPERATING PROCEDURE MICROBIOLOGY DEPARTMENT TITLE : OPERATION OF ZONE READER

REF. NO. SUPERSEDES ISSUE NO.

SOP/MIC/026 -1.0

REV. NO.

0.0

EFFECTIVE DATE REVIEW DATE PAGE NO. ID NO:

13.12.2010 13.11.2013 1 of 1 PPL/INST/ZR/026

PREPARED BY: HOD

VERIFIED BY: TQM

AUTHORISED BY: TQM

(Mr M.M.Sonnad)

(Mr. V.G. Nagwekar)

(Mr. V.G. Nagwekar)

PARALAB PRIVATE LIMITED

REF. NO. SUPERSEDES ISSUE NO.

SOP/MIC/028 -1.0

1.0

OBJECTIVE : To lay down a procedure for the operation of Colony Counter SCOPE : This procedure is applicable for operation of Colony Counter ID No : PPL/INST/CC/028 Make : Kumar Location : Microbiology Department RESPONSIBILITY : HOD shall be overall responsible for the operation of Colony Counter

2.0

3.0

4.0 PROCEDURE : 4.1 OPERATION 4.1.1 Ensure that the instrument is clean & free from dust. Magnifying lens is fixed on its appropriate plate & marker is connected to either right or left side of the instrument. 4.1.2 Switch ON the mains. 4.1.3 Switch on the power button. The internal light will be flashed ON. Again pressing this button will turn the power OFF. 4.1.4 Place the plat containing colories to be counted 4.1.5 With the help of magnifying glass count the colories and record. 4.1.6 When the work as over clean the colory counter with dettol. 4.1.7 Switch off the instrumrent when not required. 5.0 ABBREVIATION  SOP : Standard Operating Procedure 6.0 REFERENCE Operating Manual

PARALAB PRIVATE LIMITED Goregaon (e) Goregaon (e) MUMBAI - 400063 MUMBAI - 400063 STANDARD OPERATING PROCEDURE
STANDARD OPERATING PROCEDURE MICROBIOLOGY DEPARTMENT MICROBIOLOGY DEPARTMENT TITLE : OPERATION OF PAN ANALYTICAL BALANCE TITLE : OPERATION OF COLONY COUNTER

REF. NO. SUPERSEDES ISSUE NO.

SOP/MIC/029 -1.0

REV. NO. REV. NO. EFFECTIVE DATE EFFECTIVE DATE REVIEW DATE REVIEW DATE PAGE NO. ID NO: PAGE NO. ID NO:

0.0 0.0 13.12.2010 13.12.2010 13.11.2013 13.11.2013 1 of 1 PPL/INST/PBAL/029 1 of 1 PPL/INST/CC/028

PREPARED BY: HOD

VERIFIED BY: TQM

AUTHORISED BY: TQM

(Mr M.M.Sonnad)

(Mr. V.G. Nagwekar)

(Mr. V.G. Nagwekar)

PARALAB PRIVATE LIMITED

REF. NO. SUPERSEDES ISSUE NO.

SOP/MIC/030 -1.0

PREPARED BY: HOD

VERIFIED BY: TQM

AUTHORISED BY: TQM

(Mr M.M.Sonnad)

(Mr. V.G. Nagwekar)

(Mr. V.G. Nagwekar)

1.0

OBJECTIVE : To lay down a procedure for the operation of Pan Analytical Balance SCOPE : This procedure is applicable for operation of Pan Analytical Balance ID No : PPL/INST/PBAL/029 Make : Topsons Location : Microbiology Department RESPONSIBILITY : HOD shall be overall responsible for the operation of Pan Analytical Balance

2.0

3.0

4.0 4.1 4.1.1 4.1.2 4.1.3 4.1.4 4.1.5 4.1.6

PROCEDURE : OPERATION Ensure that the instrument is clean & free from dust Adjust the spirit level provided on the top of the balance for precise weighing. Clean the weighing pan before and after weighing. Ensure that the setting knobs are in the zero position. Switch ON the mains. For checking position move the knob, on the left side panel at bottom of the instrument, turn half anti clockwise direction, and when turn on the clockwise direction gives the weighing position. 4.1.7 First turn the operating knob in the checking position. The light source will be on and check that the zero zero of the vernier scale and projection scale are coinciding with one another. 4.1.8 If it does not coincide, adjust with the adjusting knob provided on the right side panel of the instrument. 4.1.9 First check in the approximate weight in its checking position, if it within the desired range then bring same knob first in the rest position and then turn the knob on its weighing position. During weighing close the weighing cabinet for more accuracy. 4.1.10 Note down the accurate weight and put the operating knob in its rest position and set all weight adjustment knobs at zero position before switching off the instrument. 4.1.11 The balance has the capacity to produce weight up to 200gm.

5.0 ABBREVIATION  SOP : Standard Operating Procedure  gm : Gram 6.0 REFERENCE Operating Manual

Goregaon (e)
REV. NO. 0.0

MUMBAI - 400063
STANDARD OPERATING PROCEDURE MICROBIOLOGY DEPARTMENT
EFFECTIVE DATE REVIEW DATE PAGE NO. 13.12.2010 13.11.2013 1 of 1 PPL/INST/LF/030

TITLE : OPERATION OF LAMINAR FLOW 1.0 OBJECTIVE : ID NO: To lay down a procedure for the operation of Laminar Flow 2.0

SCOPE : This procedure is applicable for operation of Laminar Flow ID No : PPL/INST/LF/030 Make : HMG Location : Microbiology Department RESPONSIBILITY : HOD shall be overall responsible for the operation of Laminar Flow

3.0

4.0 4.1 4.1.1 4.1.2 4.1.3

PROCEDURE : OPERATION Clean the working table,top & side glass with IPA 70% before & after use. Clean the tubelight with clean dry sterile duster. Check the level of the instrument base. The base should possess uniform level with no ups & downs. 4.1.4 Check Air Flow pressure manometer indicate within acceptance limit [12 to 17 mm for Microroom, 10 to 15 mm for Sterility Room] 4.1.5 Start the laminar flow to allow the flow of sterile air. 4.1.6 Continue the work after half an hour of starting the laminar flow. 4.1.7 Switch on the tubelights of the laminar flow bench. 4.1.8 Bring only required sterile utensils & glass wares to the LAF bench. 4.1.9 Start gas burners. 4.1.10 Carry out microbiological work near the flame of gas burner. 4.1.11 Do not do any writing work in the Laminar Flow. 4.1.12 Immediately remove plates, glass ware & media from the area. 4.1.13 Clean the working area.Spray the disinfectant solution. 4.1.14 Switch OFF the tube lights of LAF bench.Switch OFF the instrument when not in use.

5.0 ABBREVIATION  SOP : Standard Operating Procedure  mm : Millimeter 6.0 REFERENCE Operating Manual

PREPARED BY: HOD

VERIFIED BY: TQM

AUTHORISED BY: TQM

(Mr M.M.Sonnad)

(Mr. V.G. Nagwekar)

(Mr. V.G. Nagwekar)

PARALAB PRIVATE LIMITED Goregaon (e) MUMBAI - 400063

STANDARD OPERATING PROCEDURE MICROBIOLOGY DEPARTMENT TITLE : OPERATION OF LAMINAR FLOW

REF. NO. SUPERSEDES ISSUE NO.

SOP/MIC/031 -1.0

REV. NO.

0.0

EFFECTIVE DATE REVIEW DATE PAGE NO. ID NO:

13.12.2010 13.11.2013 1 of 1 PPL/INST/LF/031

OBJECTIVE : OBJECTIVE To lay down a :procedure for the operation of Laminar Flow To lay down a procedure for the operation of Cyclo Mixer 2.0 SCOPE : 2.0 SCOPE : This procedure is applicable for operation of Laminar Flow This procedure is applicable Make : HMG Cyclo Mixer ID No : PPL/INST/LF/ 031 for operation of Location : Microbiology Department ID No : PPL/INST/CYM/032 Make : Remi Location : Microbiology Department 3.0 RESPONSIBILITY : 3.0 RESPONSIBILITY :responsible for the operation of Laminar Flow HOD shall be overall HOD shall be overall responsible for the operation of Cyclo Mixer 4.0 PROCEDURE : 4.0 OPERATION : PROCEDURE 4.1 4.1 OPERATION 4.1.1 Clean the working table,top & side glass with IPA 70% before & after use. 4.1.1 Switch ON the mains. clean dry sterile duster. 4.1.2 Clean the tubelight with 4.1.2 Adjust the speed by rotating the knob in The base should possess uniform level with no ups & 4.1.3 Check the level of the instrument base. clockwise /anticlockwise direction according to the requirement. And then start. downs. 4.1.3 Switch OFF the pressure manometer indicate within acceptance limit [12 to 17 mm for 4.1.4 Check Air Flow mains when not in use for a long period 4.1.4 Hold test tubes properly and tightly on the mixer Microroom, 10 to 15 mm for Sterility Room] 4.1.5 Start the laminar flow to allow the flow of sterile air. 5.0 ABBREVIATION 4.1.6 Continue the work after half an hour of starting the laminar flow.  SOP : Standard Operating Procedure 4.1.7 Switch on the tubelights of the laminar flow bench. 4.1.8 Bring only required sterile utensils & glass wares to the LAF bench. 6.0 REFERENCE 4.1.9 Start gas burners. Operating Manual 4.1.10 Carry out microbiological work near the flame of gas burner. 4.1.11 Do not do any writing work in the Laminar Flow. 4.1.12 Immediately remove plates, glass ware & media from the area. 4.1.13 Clean the working area.Spray the disinfectant solution. 4.1.14 Switch OFF the tube lights of LAF bench.Switch OFF the instrument when not in use. 1.0 1.0 5.0 ABBREVIATION  SOP : Standard Operating Procedure  mm : Millimeter 6.0 REFERENCE Operating Manual
VERIFIED BY: TQM AUTHORISED BY: TQM

PREPARED BY: HOD

(Mr M.M.Sonnad)

(Mr. V.G. Nagwekar)

(Mr. V.G. Nagwekar)

PARALAB PRIVATE LIMITED Goregaon (e) MUMBAI - 400063

STANDARD OPERATING PROCEDURE MICROBIOLOGY DEPARTMENT TITLE : OPERATION OF CYCLO MIXER LAMINAR FLOW

REF. NO. SUPERSEDES ISSUE NO.

SOP/MIC/035 SOP/MIC/032 -1.0

REV. NO.

0.0

EFFECTIVE DATE REVIEW DATE PAGE NO. ID NO:

13.12.2010 13.11.2013 1 of 1 PPL/INST/CYM/032 PPL/INST/LF/035

PREPARED BY: HOD

VERIFIED BY: TQM

AUTHORISED BY: TQM

(Mr M.M.Sonnad)

(Mr. V.G. Nagwekar)

(Mr. V.G. Nagwekar)

PARALAB PRIVATE LIMITED

REF. NO. SUPERSEDES ISSUE NO.

SOP/MIC/036 -1.0

PREPARED BY: HOD

VERIFIED BY: TQM

AUTHORISED BY: TQM

(Mr M.M.Sonnad)

(Mr. V.G. Nagwekar)

(Mr. V.G. Nagwekar)

1.0

OBJECTIVE : To lay down a procedure for the operation of Laminar Flow SCOPE : This procedure is applicable for operation of Laminar Flow ID No : PPL/INST/LF/035 Make : HMG Location : Microbiology Department RESPONSIBILITY : HOD shall be overall responsible for the operation of Laminar Flow

2.0

3.0

4.0 PROCEDURE : 4.1 OPERATION 4.1.15 Clean the working table,top & side glass with IPA 70% before & after use. 4.1.16 Clean the tubelight with clean dry sterile duster. 4.1.17 Check the level of the instrument base. The base should possess uniform level with no ups & downs. 4.1.18 Check Air Flow pressure manometer indicate within acceptance limit [12 to 17 mm for Microroom, 10 to 15 mm for Sterility Room] 4.1.19 Start the laminar flow to allow the flow of sterile air. 4.1.20 Continue the work after half an hour of starting the laminar flow. 4.1.21 Switch on the tubelights of the laminar flow bench. 4.1.22 Bring only required sterile utensils & glass wares to the LAF bench. 4.1.23 Start gas burners. 4.1.24 Carry out microbiological work near the flame of gas burner. 4.1.25 Do not do any writing work in the Laminar Flow. 4.1.26 Immediately remove plates, glass ware & media from the area. 4.1.27 Clean the working area.Spray the disinfectant solution. 4.1.28 Switch OFF the tube lights of LAF bench.Switch OFF the instrument when not in use. 5.0 ABBREVIATION  SOP : Standard Operating Procedure  mm : Millimeter 6.0 REFERENCE Operating Manual

Goregaon (e)
REV. NO. 0.0

MUMBAI - 400063
STANDARD OPERATING PROCEDURE MICROBIOLOGY DEPARTMENT
EFFECTIVE DATE REVIEW DATE PAGE NO. 13.12.2010 13.11.2013 1 of 1 PPL/INST/ACV/036

TITLE : OPERATION OF AUTOCLAVE (VERTICAL) 1.0 OBJECTIVE : ID NO: To lay down a procedure for the operation of Autoclave (Vertical) 2.0 SCOPE : This procedure is applicable for operation of Autoclave (Vertical) ID No : PPL/INST/ACV/036 Make : Modern Scientific Location : Microbiology Department RESPONSIBILITY : HOD shall be overall responsible for the operation of Autoclave (Vertical)

3.0

PROCEDURE : OPERATION Ensure that the autoclave is clean & free form dust & placed in a safe area. Fill the autoclave with D.M. water till the heater is immersed fully in the water. Load the article to be sterilized in the basket. Shut the lid by help of lifter & close the autoclave. Tight the screws diagonally. Switch ON the mains. Open the steam release valve & release the steam for 5 min., to avoid vacuum in the chamber. Close the valve. 4.1.8 Continue heating till the pressure gauge shows 15lbs/In2 pressure & temperature 121C srC constant. Keep it for the time specified or required for sterilization. 4.1.9 Switch OFF the mains, release the steam by opening valve slowly till pressure gauge declined to zero. 4.1.10 Loose the screw knob & lift up the lid by lifter. 4.1.11 Remove the loaded article by using the gloves. 4.1.12 Put one signaloc strip indicator in the basket along with the article to be sterilized. 4.1.13 After sterilization is over, remove the signaloc strip indicator & check its change in colour, proves the proper functioning of the autoclave 4.0 4.1 4.1.1 4.1.2 4.1.3 4.1.4 4.1.5 4.1.6 4.1.7 5.0 ABBREVIATION  SOP : Standard Operating Procedure  min : Minute  lbs/In2 : Pounds per square inch 6.0 REFERENCE Operating Manual

PREPARED BY: HOD

VERIFIED BY: TQM

AUTHORISED BY: TQM

(Mr M.M.Sonnad)

(Mr. V.G. Nagwekar)

(Mr. V.G. Nagwekar)

PARALAB PRIVATE LIMITED Goregaon (e) MUMBAI - 400063

STANDARD OPERATING PROCEDURE MICROBIOLOGY DEPARTMENT TITLE : OPERATION OF AUTOCLAVE (VERTICAL)

REF. NO. SUPERSEDES ISSUE NO.

SOP/MIC/037 -1.0

REV. NO.

0.0

EFFECTIVE DATE REVIEW DATE PAGE NO. ID NO:

13.12.2010 13.11.2013 1 of 1 PPL/INST/ACV/037

PARALAB PRIVATE LIMITED

REF. NO. SUPERSEDES ISSUE NO.

SOP/MIC/039 -1.0

PREPARED BY: HOD

VERIFIED BY: TQM

AUTHORISED BY: TQM

(Mr M.M.Sonnad)

(Mr. V.G. Nagwekar)

(Mr. V.G. Nagwekar)

1.0 1.0 2.0 2.0

OBJECTIVE : OBJECTIVE : To lay down a procedure for the operation of Refrigerator To lay down a procedure for the operation of Autoclave (Vertical) SCOPE : SCOPE : This procedure is applicable for operation of Refrigerator This procedure is applicable for operation of Autoclave (Vertical) ID No : PPL/INST/ACV/037 Make : : Modern Location : Microbiology Department ID No : PPL/INST/REF/039 Make Godrej Scientific Location : Microbiology Department RESPONSIBILITY : RESPONSIBILITY : HOD shall be overall responsible for the operation of Autoclave (Vertical) HOD shall be overall responsible for the operation of Refrigerator

3.0 3.0

4.0 PROCEDURE : 4.1 OPERATION : 4.0 PROCEDURE 4.1.13 OPERATION autoclave is clean & free form dust & placed in a safe area. 4.1 Ensure that the 4.1.14 Switch autoclave with D.M. water till the shall is immersed temperature. 4.1.1 Fill the on the main supply, digital displayheaterdisplay actualfully in the water. 4.1.15 Store all article to preparation in the basket. 4.1.2 Load the the mediabe sterilized with label on it describing name of media, product for which 4.1.16 Shutprepared, date of preparation, expiry date and name of individual who have prepared the it is the lid by help of lifter & close the autoclave. 4.1.17 media. screws diagonally. Tight the 4.1.18 Set the temperature by choosing three options inside the fridge i.e. Max, Normal-min and 4.1.3 Switch ON the mains. 4.1.19 off. the steam release valve & release the steam for 5 min., to avoid vacuum in the Open chamber. Close the valve. 4.1.20 ABBREVIATION the pressure gauge shows 15lbs/In2 pressure & temperature 121C srC Continue heating till 5.0  SOPconstant. Keep it for the time specified or required for sterilization. : Standard Operating Procedure 4.1.21 Switch OFF the mains, release the steam by opening valve slowly till pressure gauge declined to zero. 6.0 REFERENCE 4.1.22 Operating screw knob & lift up the lid by lifter. Loose the Manual 4.1.23 Remove the loaded article by using the gloves. 4.1.24 Put one signaloc strip indicator in the basket along with the article to be sterilized. 4.1.14 After sterilization is over, remove the signaloc strip indicator & check its change in colour, proves the proper functioning of the autoclave 5.0 ABBREVIATION  SOP : Standard Operating Procedure  min : Minute  lbs/In2 : Pounds per square inch 6.0 REFERENCE Operating Manual

PARALAB PRIVATE LIMITED Goregaon (e) Goregaon (e) MUMBAI - 400063 MUMBAI - 400063 STANDARD OPERATING PROCEDURE
STANDARD OPERATING PROCEDURE MICROBIOLOGY DEPARTMENT MICROBIOLOGY DEPARTMENT TITLE : OPERATION OF INCUBATOR TITLE : OPERATION OF REFRIGERATOR

REF. NO. SUPERSEDES ISSUE NO.

SOP/MIC/040 -1.0

REV. NO. REV. NO. EFFECTIVE DATE EFFECTIVE DATE REVIEW DATE REVIEW DATE PAGE NO. ID NO: PAGE NO. ID NO:

0.0 0.0 13.12.2010 13.12.2010 13.11.2013 13.11.2013 1 of 1 PPL/INST/INC/040 1 of 1 PPL/INST/REFR/

PREPARED BY: HOD

VERIFIED BY: TQM

AUTHORISED BY: TQM

(Mr M.M.Sonnad)

(Mr. V.G. Nagwekar)

(Mr. V.G. Nagwekar)

PARALAB PRIVATE LIMITED

REF. NO. SUPERSEDES ISSUE NO.

SOP/MIC/041 -1.0

PREPARED BY: HOD

VERIFIED BY: TQM

AUTHORISED BY: TQM

(Mr M.M.Sonnad)

(Mr. V.G. Nagwekar)

(Mr. V.G. Nagwekar)

1.0 1.0 2.0 2.0

3.0 3.0 4.0 4.0 4.1 4.1 4.1.1 4.1.1 4.1.2 4.1.2 4.1.3 4.1.3 4.1.4 4.1.4 4.1.5 4.1.5

OBJECTIVE : OBJECTIVE To lay down a :procedure for the operation of Incubator To lay down a procedure for the operation of Incubator SCOPE : SCOPE : This procedure is applicable for operation of Incubator This procedure is applicable for operation of Incubator : Microbiology Department ID No : PPL/INST/INC/040 Make : Tropical Location ID No : PPL/INST/INC/041 Make : Tropical Location : Microbiology Department RESPONSIBILITY : RESPONSIBILITY :responsible for the operation of Incubator HOD shall be overall HOD shall be overall responsible for the operation of Incubator PROCEDURE : PROCEDURE OPERATION : OPERATION Switch on the main supply, digital display shall display actual temperature. Switch required temperature by pressing the red coloured push switch. Now the display shall Set the on the main supply, digital display shall display actual temperature. Set the required temperature by pressing thepressed uses can set desired temperature with the show temperature vaine keeping the switch red coloured push switch. Now the display shall showof coarse and vaine keeping the switch pressed uses can set desired temperature with the help temperature finally with the help of fine adjustment. help of coarse and switch; with thetemperature adjustment. Release the Red finally actual help of fine will be displayed, which should match with Release the on thermometer kept inside the incubator. displayed, which should match with temperature Red switch; actual temperature will be temperature on thermometer kept inside the incubator. Temperature record should be maintained daily. Temperature record should be maintained daily. Temperature deviation accepted upto +/- 1C. Temperature deviation accepted upto +/- 1C.

5.0 ABBREVIATION 5.0  SOPABBREVIATION Procedure : Standard Operating  SOP : Standard Operating Procedure 6.0 6.0 REFERENCE REFERENCE Operating Manual Operating Manual

PARALAB PRIVATE LIMITED Goregaon (e) Goregaon (e) MUMBAI - 400063 MUMBAI - 400063 STANDARD OPERATING PROCEDURE
STANDARD OPERATING PROCEDURE MICROBIOLOGY DEPARTMENT MICROBIOLOGY [BIOLOGICAL TITLE : OPERATION OF B.O.D.DEPARTMENTOXYGEN DEMAND] INCUBATOR TITLE : OPERATION OF INCUBATOR

REF. NO. SUPERSEDES ISSUE NO.

SOP/MIC/043 -1.0

REV. NO. REV. NO. EFFECTIVE DATE EFFECTIVE DATE REVIEW DATE REVIEW DATE PAGE NO. ID NO: PAGE NO. ID NO:

0.0 0.0 13.12.2010 13.12.2010 13.11.2013 13.11.2013 1 of 1 PPL/INST/BOD/043 1 of 1 PPL/INST/INC/041

PREPARED BY: HOD

VERIFIED BY: TQM

AUTHORISED BY: TQM

(Mr M.M.Sonnad)

(Mr. V.G. Nagwekar)

(Mr. V.G. Nagwekar)

PARALAB PRIVATE LIMITED

REF. NO. SUPERSEDES ISSUE NO.

SOP/MIC/042 -1.0

PREPARED BY: HOD

VERIFIED BY: TQM

AUTHORISED BY: TQM

(Mr M.M.Sonnad)

(Mr. V.G. Nagwekar)

(Mr. V.G. Nagwekar)

1.0

OBJECTIVE : To lay down a procedure for the operation of B.O.D Incubator SCOPE : This procedure is applicable for operation of B.O.D Incubator ID No : PPL/INST/BOD/043 Make : Spectrum Location : Microbiology Department RESPONSIBILITY : HOD shall be overall responsible for the operation of B.O.D Incubator PROCEDURE : OPERATION Switch on the mains switch and B.O.D. Red main switch. Press black button and adjust required temperature with help of Reset and zero knob. Release the black button, actual temperature will display. Check the temperature on digital display, should match with temperature on thermometer, which is kept inside the incubator. For cooling time compressor will on after 90 sec. period Heating and cooling will be indicated by Red & Green colour lamp respectively, provided on front panel. Check the actual temperature after sometime. Temperature record should be maintained daily. Temperature deviation accepted upto +/- 1C.

2.0

3.0

4.0 4.1 4.1.1 4.1.2 4.1.3 4.1.4 4.1.5 4.1.6 4.1.7 4.1.8 4.1.9

5.0 ABBREVIATION  SOP : Standard Operating Procedure  B.O.D. : Biological Oxygen Demand  sec : Second 6.0 REFERENCE Operating Manual

Goregaon (e)
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MUMBAI - 400063
STANDARD OPERATING PROCEDURE MICROBIOLOGY DEPARTMENT TITLE : OPERATION OF HOT AIR OVEN 1.0
EFFECTIVE DATE REVIEW DATE PAGE NO. ID NO: 13.12.2010 13.11.2013 1 of 1 PPL/INST/HAO/042

OBJECTIVE : To lay down a procedure for the operation of Hot Air Oven SCOPE : This procedure is applicable for operation of Hot Air Oven ID No : PPL/INST/HAO/042 Make : Modern Scientific Location : Microbiology Department RESPONSIBILITY : HOD shall be overall responsible for the operation of Hot Air Oven

2.0

3.0

4.0 PROCEDURE : 4.1 OPERATION 4.1.1 Switch ON the mains. 4.1.2 The green indication light will be ON. 4.1.3 There is three-way switch for the selection of temperature range. 4.1.4 The requied temperature (e.g.105C) can be set by a thermostatically controlled knob, by rotating it in clockwise or anti-clockwise direction. 4.1.5 Note : When temperature is increased, a red indication light will be ON & then will be OFF automatically. 4.1.6 Inside the oven, there are two trays. Put any object that requires drying , on this tray & close the door of the oven. 4.1.7 A small hole is provided on the top of the instrument, to place thermometer or any other temperature measuring device. 4.1.8 A vapour or fumes releasing valve is also provided on the top or side of the instrument. 4.1.9 Switch OFF the instrument & mains, when not in use for long period. 5.0 ABBREVIATION  SOP : Standard Operating Procedure 6.0 REFERENCE Operating Manual

PREPARED BY: HOD

VERIFIED BY: TQM

AUTHORISED BY: TQM

(Mr M.M.Sonnad)

(Mr. V.G. Nagwekar)

(Mr. V.G. Nagwekar)