Code No: RR322304 Set No.

1
III B.Tech Supplimentary Examinations, Aug/Sep 2008
BIOPROCESS ENGINEERING-II
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Write short notes on: [16]

(a) The aeration system

(b) The agitator
(c) Baffles
(d) foam control.

2. Explain about Tracer pulse experiment to calculate conversion with respect to

tanks-in-series model? [16]

3. List out the cardinal rules to be followed in design of a fermentor and its construc-
tion materials for successful operation? [16]

4. Write about the techniques employed in On-line analysis of Chemical factors? [16]

5. (a) Discuss in detail about ON-Line Sensor.

(b) Discuss in detail about OFF-Line Analytical Methods [8+8]

6. Explain in detail about the various stages in gene expression. [16]

7. Discuss in detail about protein Engineering. [16]

8. Discuss in detail about a highly structured single-cell model for growth of E.Coli
on glucose-ammonium salts medium. [16]

⋆⋆⋆⋆⋆

1 of 1
Code No: RR322304 Set No. 2
III B.Tech Supplimentary Examinations, Aug/Sep 2008
BIOPROCESS ENGINEERING-II
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Explain the basis for classification of Bioreactors and write in brief about them
with an example? [16]

2. Write short notes on: [16]

(a) Non-Ideal Tubular reactor

(b) Non-Ideal CSTR.

3. List out the cardinal rules to be followed in design of a fermentor and its construc-
tion materials for successful operation? [16]

4. Write in detail about the methods employed in measuring the following process
variables. [8+8]

(a) Temperature
(b) Pressure.

5. Explain the advantages gained by coupling process instruments to digital comput-

ers. [16]

6. Discuss in detail the control and information sequences in DNA guide the tran-
scription and transaation process which result in gene expression. [16]

7. Write Short Notes On: [16]

(a) Monoclonal antibodies.

(b) Immunobiological Regulators.
(c) Virus Vaccines.
(d) Hormones.

8. Explain unit impulse response model. [16]

⋆⋆⋆⋆⋆

1 of 1
Code No: RR322304 Set No. 3
III B.Tech Supplimentary Examinations, Aug/Sep 2008
BIOPROCESS ENGINEERING-II
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. How cells grow in Continuous culture ? Explain about some specific devices em-
ployed for continuous culture ? [16]

2. Explain in detail the design of Continuous sterilization process ? [16]

3. List out the cardinal rules to be followed in design of a fermentor and its construc-
tion materials for successful operation? [16]

4. How can you determine [6+4+6]

(a) Cellular ATP content

(b) Single cell characteristics
(c) Assay of cells with and without plasmids.

5. (a) Discuss in detail about ON-Line Sensor.

(b) Discuss in detail about OFF-Line Analytical Methods [8+8]

6. Explain in detail about the various stages in gene expression. [16]

7. Discuss in detail about the formation of a hybridoma for production of monoclonal

antibodies. [16]

8. Discuss in detail about a Morphologically structured kinetic model for Cephalosporin

C production. [16]

⋆⋆⋆⋆⋆

1 of 1
Code No: RR322304 Set No. 4
III B.Tech Supplimentary Examinations, Aug/Sep 2008
BIOPROCESS ENGINEERING-II
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. What are the different types of the bioreactors employed in Animal Cell Culture ?
Explain about packed glass bead reactors and Perfusion cultures ? [16]

2. Write short notes on: [8+8]

(a) One-parameter model

(b) Two-parameter model

3. Write short notes on: [8+8]

(a) Fed-batch reactors

(b) Enzyme catalyzed reactions in CSTR.

4. Write in detail about the methods employed in measuring the following process
variables. [8+8]

(a) Temperature
(b) Pressure.

5. (a) Discuss in detail about ON-Line Sensor.

(b) Discuss in detail about OFF-Line Analytical Methods [8+8]

6. Explain Holistic perspective on heterologous protein production with the bac-

ulovirus expression system. [16]

7. Discuss in detail about product formation kinetics. [16]

8. Discuss in detail about a Morphologically structured kinetic model for Cephalosporin

C production. [16]

⋆⋆⋆⋆⋆

1 of 1