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SFX GREENHERALD INTERNATIONAL SCHOOL BIOLOGY MCQ TEST FOR AS LEVEL Chapters: Microscopy Name: Time: 20 minutes

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(For MCQs Place a CIRCLE over the alphabet of the corresponding correct answer) 1. What describes resolution in microscopy? A the ability to distinguish between two objects that are very close together B the clarity of the image formed by the microscope C the number of times the image has been magnified by the objective lens D the power of the microscope to focus on very small objects 2. What is compound microscope? A Microscope with the capability to view oil immersion B Microscope with the capability to view compounds C Microscope with a single lens D Microscope with two lenses

3. What is the total magnification achieved with a compound microscope? A Magnification of objective lens B Magnification of ocular lens C Magnification of ocular lens added to the magnification of the objective lens D Magnification of ocular lens multiplied by the magnification of the objective lens

4. What is another name for the light microscope? A Simple microscope B Compound microscope C Phase contrast microscope

D Dissection microscope 5. Which microscope would be particularly useful for looking at living cells? A Simple microscope B Compound microscope C Phase contrast microscope D Transmission electron microscope

6. Which type of microscope has only one lens? A Simple microscope B Compound microscope C Phase contrast microscope D Dissection microscope 7. What is the resolution, in nanometres, of an electron microscope and of a light microscope? electron microscope A B C D 0.5 0.5 5.0 5.0 light microscope 20 200 20 200

8. What describes the features of an electron microscope? maximum magnification A B C D 2.5 ×103 2.5 ×104 2.5 ×105 5.0 ×105 resolution / nm 2.5 ×102 5.0 ×10-1 5.0 ×10-1 2.5 ×102 specimen dead living dead living

9. Which cell structure can be seen only with an electron microscope? A cell surface membrane B cell wall C chromosome

D nucleolus .

what effect will this have on the magnification and on the resolution of the microscope? magnification A B C D decreased increased remains the same remains the same resolution decreased increased increased decreased . Pollen grains were grown in a sugar solution and viewed using the eyepiece graticule. The diagram shows a graduated slide. A specimen is viewed under a microscope using green light with a wavelength of 510 nm. Diagram 1 shows the pollen grains at first and diagram 2 shows them after four hours.10.1 mm viewed using an eyepiece graticule. If the same specimen is viewed under the same conditions. with divisions of 0. but using red light with a wavelength of 650 nm instead. diagram 1 diagram 2 at start What is the growth rate of the pollen tubes? A 5 µmh-1 B 10 µmh-1 C 5 mmh-1 D 10 mmh-1 after 4 hours 11.

The diagram shows a photomicrograph. Its magnification is ×2800. seen under a light (optical) microscope at ×400 magnification? C 13. Which represents the same cell. The diagram below is drawn from an electron micrograph of an animal cell.12.5 µm B 5 µm C 10 µm D 20 µm . What is the diameter of the nucleolus? A 2.

of a palisade mesophyll cell. The diagram shows a stage micrometer on which the small divisions are 0. 2 and 3 B 1. 3 and 4 . 2 and 4 C 1. The student observed 1 the cisternae of the Golgi apparatus 2 the grana in the chloroplasts 3 the two membranes of the nuclear envelope 4 the vacuole enclosed by a tonoplast Which features can be seen because of the higher resolution of the electron microscope? A 1.14. one using a high quality light microscope and the other using an electron microscope. taken at the same magnification. The stage micrometer is replaced by a slide of a plant cell. It is viewed through an eyepiece containing a graticule. A student is asked to study two photographs. What is the width of a chloroplast? A 5 μm B 10 μm C 50 μm D 100 μm 15.1 mm. 3 and 4 D 2.

Which eyepiece and objective lens combination enables you to see the greatest number of cells in the field of view? eyepiece A B C D ×5 ×10 ×5 ×10 objective ×10 ×10 ×40 ×40 17. The graticule and stage micrometer are used to measure cells. Which is the correct reason why the graticule calibrated? A The graticule can be used to make measurements. viewed through an eyepiece containing a graticule. with divisions 0. 18.16. How many graticule divisions will cover the diameter of a white cell of 10 µm? A1 B4 C 10 D 20 . D The graticule makes comparisons. The same eyepiece is now used to examine a blood smear. B The graticule is magnified by the objective lens.1 mm apart. C The graticule magnifies the specimen. The diagram shows a stage micrometer.

3 and 4 only C 1 and 2 only D 3 and 4 only THE END . 4 Multiply the number of eyepiece graticule units by the calibration of the eyepiece graticule. Which steps are needed to find the actual width of a xylem vessel viewed in transverse section using a ×40 objective lens? 1 Convert from mm to µm by multiplying by 10–3. If the same specimen is viewed under the same conditions.19. A specimen is viewed under a microscope using green light with a wavelength of 510 nm. 2 Calibrate the eyepiece graticule using a stage micrometer on ×10 objective lens. A 1. 3 and 4 B 2. what effect will this have on the magnification and on the resolution of the microscope? magnification A B C D decreased increased remains the same remains the same resolution decreased increased decreased increased 20. but using red light with a wavelength of 650 nm instead. 2. 3 Measure the width of the xylem vessel using an eyepiece graticule.

Questionnaire prepared by: Md. Emrul Hasan. SFX Greenherald Int’l School . Teacher (Biology).

1000X 2. 100X e. objective lenses can be found to have a magnification of all of the following. 0. What is this phenomenon called? a. What is "compound microscope"? a. What is the turret? a. Unifocal c. EXCEPT? a. Microscope with the capability to view oil immersion b. Tube e. On a microscope. Magnification of ocular lens multiplied by the magnification of the objective lens e. 0. 2 angstroms 5. Magnification of ocular lens c. Microscope with a single lens d. What is the total magnification achieved with a compound microscope? a. Diaphragm 7. Base b. What is the maximum resolving power seen with a compound microscope? a. what structure connects the eyepiece to the objective lens? a. Diaphragm b. Stage d. the focus knob does not have to be readjusted when changing the magnification. 2 millimeters c. 10X c. In a good compound microscope.2 millimeters d. 4X b. Microscope with the capability to view compounds c. 2 micrometers e. Microscope with two lenses e. Nosepiece c. Focused e. Magnification of ocular lens added to the magnification of the objective lens d. 40X d. Base b. Microscope with three lenses 3. Stage d. Magnification of objective lens b. Parfocal b. Convergent . Magnification of condenser lens multiplied by the magnification of the objective lens 4. Bifocal d. Tube e. Nosepiece c.2 micrometers 6.1. When using a compound microscope.

Phase contrast microscope e. Which microscope does not rely on visible light? a. Zero b. Phase contrast microscope e. Simple microscope c. 10X c. 1000X 12. On a microscope. as glass d. 10X c. 100X d.8. Phase contrast microscope e. What is another name for the light microscope? a. what is the refractive index of the oil? a. What is the usual magnification of the ocular lens on a compound microscope? a. as air c. what is the magnification of the oil immersion lens? a. as water e. Transmission electron microscope b. Focus the light on the specimen e. Simple microscope c. Compound microscope d. Dissection microscope 9. what structure varies the diameter of the cone of light? b. What is the role of the condenser lens? a. When using a compound microscope. 1X b. When using oil immersion to view a tissue. None 14.000 X 13. Control the aperture of light c. Which microscope makes things appear three dimensional? a. Transmission electron microscope b. Compound microscope d. 100X e. Dissection microscope 11. Transmission electron microscope b. 40X d. 1000X e. Initial magnification of 10X . Compound microscope d. Provide light 15. Dissection microscope 10. Increase the magnification d. Simple microscope c. 4X b. 10.

Dissection microscope b. Which type of microscope has only one lens? a. Compound microscope d. Emrul Hasan. Tube e. Compound microscope d. Transmission electron microscope THE END Questionnaire prepared by: Md. Compound microscope d. SFX Greenherald Int’l School b. Base b. Stage d. Phase contrast microscope e. Where do you place the slide when using a microscope? a. Dissection microscope b. Tube e. Diaphragm 17. Nosepiece c. Simple microscope c. Nosepiece c. Teacher (Biology). Transmission electron microscope 19. Base b. Tube e. Which microscope would be particularly useful for looking at living cells? a. Stage d. Nosepiece c. Phase contrast microscope e. Base b.a. Stage d. Transmission electron microscope 20. What is the bottom of a microscope called? a. Simple microscope c. Diaphragm 16. What is another name for the bright field microscope? a. Dissection microscope . Diaphragm 18. Simple microscope c. Phase contrast microscope e.

c 20.Answer Key to Questions of Microscopy 1. 10X c. The eyepiece lens usually has a magnification of 10X. a 18. d 7. e 16. 1000X Answer: e A compound microscope has two lenses: an eyepiece lens and objective lens. 2. b 9. EXCEPT? a. Some compound microscopes also have an oil immersion lens with a magnification of 100X. Microscope with a single lens d. b 13. Microscope with the capability to view oil immersion b. Microscope with two lenses . 10X. 40X d. e 2. b 6. 100X e. objective lenses can be found to have a magnification of all of the following. b 19. c 17. d 12. a 1. e 10. When using a compound microscope. 4X b. c 14. d 5. There are objective lenses on the revolving nosepiece with varying magnifications. What is "compound microscope"? a. d 4. Most compound microscopes have objective lenses with magnification of 4X. c 15. d 3. d 11. a 8. and 40X. Microscope with the capability to view compounds c.

4.2 millimeters c. Magnification of ocular lens added to the magnification of the objective lens d. 10X. Some compound microscopes also have an oil immersion lens with a magnification of 100X. and 40X. For example. There are objective lenses on the revolving nosepiece with varying magnifications. Magnification of ocular lens c. 0. Magnification of condenser lens multiplied by the magnification of the objective lens Answer: d To calculate the total magnification achieved with a compound microscope. What is the maximum resolving power seen with a compound microscope? a. The eyepiece lens usually has a magnification of 10X. 2 micrometers d. the magnification of the ocular lens is multiplied by the magnification of the objective lens. 2 millimeters b.e. What is the total magnification achieved with a compound microscope? a. Most compound microscopes have objective lenses with magnification of 4X. Magnification of objective lens b. if viewing a sample with the 40x objective. Magnification of ocular lens multiplied by the magnification of the objective lens e. Microscope with three lenses Answer: d A compound microscope has two lenses: an eyepiece lens and objective lens. the total magnification would be calculated as follows: a 10X ocular lens used with a 40X objective lens. 3. 0. the total magnification is 400X (10 x 40).2 micrometers .

Nosepiece c. thus. The tube is the structure which connects the eyepiece to the objective lenses (it is shaped like a tube.2 micrometers. Base b. they are perceived as one object. its name). Stage d. 2 angstroms Answer: d Resolving power is the ability to see two things as discrete images. In a good compound microscope. Diaphragm Answer: b The base is the bottom of the microscope. 6. The objective lens are attached to the nosepiece (or turret). Any closer than this. the focus knob does not have to be readjusted when changing the magnification. Stage d. Base b. What is this phenomenon called? . What is the turret? a. The diaphragm controls the diameter of the cone of light. With normal vision. Tube e. In other words. The revolving nosepiece is also called a turret. two marks 0. 7. The slide rests on the stage. there is a resolving power of about of 100 micrometers. On a microscope. The slide rests on the stage. The revolving nosepiece is also called a turret. Tube e. Diaphragm Answer: d The base is the bottom of the microscope. its name). what structure connects the eyepiece to the objective lens? a. thus. Nosepiece c. The diaphragm controls the diameter of the cone of light.2 micrometers apart can be seen as two distinct entities. The tube is the structure which connects the eyepiece to the objective lenses (it is shaped like a tube. A compound microscope has a resolving power of approximately 0. The objective lens are attached to the nosepiece (or turret).e. 5.

because the specimen does not need to be stained. the specimen remains in focus. Dissection microscope e. Focused e. Phase contrast microscope d. Compound microscope c. Things appear three dimensional with a dissection microscope. A transmission electron microscope does not use light. Simple microscope b. but rather a beam of electrons. Simple microscope b. A phase contrast microscope is useful for examining living cells. Transmission electron microscope Answer: e . Compound microscope c. Bifocal d. Which microscope does not rely on visible light? a. 9.a. Transmission electron microscope Answer: b A simple microscope has only one lens. Unifocal c. A dissection microscope uses low power magnification. A compound microscope utilizes two lenses: an ocular lens and an objective lens. The compound microscope is also referred to as a "light microscope" or "bright field microscope". This phenomenon is seen with good compound microscopes. 8. In other words. Convergent Answer: a Parafocal is the term used for a microscope if the focus knob does not have to be readjusted when changing the magnifications. when the specimen is in focus at 4X and the objective is switched to 10X. Phase contrast microscope d. Parfocal b. What is another name for the light microscope? a. Dissection microscope e.

The compound microscope is also referred to as a "light microscope" or "bright field microscope". because the specimen does not need to be stained. because the specimen does not need to be stained. A phase contrast microscope is useful for examining living cells. The compound microscope is also referred to as a "light microscope" or "bright field microscope". Simple microscope b. . 10. Compound microscope c. Things appear three dimensional with a dissection microscope. A dissection microscope uses low power magnification. A transmission electron microscope does not use light. Transmission electron microscope Answer: d A simple microscope has only one lens. but rather a beam of electrons. Things appear three dimensional with a dissection microscope. A compound microscope utilizes two lenses: an ocular lens and an objective lens. Which microscope makes things appear three dimensional? a. A compound microscope utilizes two lenses: an ocular lens and an objective lens. Dissection microscope e. A phase contrast microscope is useful for examining living cells. but rather a beam of electrons. Phase contrast microscope d. A transmission electron microscope does not use light.A simple microscope has only one lens. A dissection microscope uses low power magnification.

Same as air c. It is also called the eyepiece lens. 1X b. 13. and 40X. Zero b. what is the magnification of the oil immersion lens? a. 12. the one that you first look through when using a microscope. Some microscopes have a 15X eyepiece lens. Same as glass . Most compound microscopes have objective lenses with magnification of 4X. There are objective lenses on the revolving nosepiece with varying magnifications. 10X c. 1000X Answer: d A compound microscope has two lenses: an eyepiece lens and objective lens.11. 1000X e. 10X c. What is the usual magnification of the ocular lens on a compound microscope? a. 100X e. When using a compound microscope. 100X d. 40X d.000 X Answer: b The usual magnification of an ocular lens on a compound microscope is 10X. The ocular lens is the lens at the top of the tube. When using oil immersion to view a tissue. 10. 4X b. Some compound microscopes also have an oil immersion lens with a magnification of 100X. what is the refractive index of the oil? a. 10X. The eyepiece lens usually has a magnification of 10X.

Oil immersion replaces that space of air with oil. Focus the light on the specimen d. None of the above Answer: c With light microscopy. Provide light Answer: c The role of the condenser lens is to focus light on the specimen. Tube e. Same as water e. Initial magnification of 10X e. The refractive index of the oil is the same as glass.d. there normally is a space of air between the slide and the lens. It is used with higher magnifications. On a microscope. Nosepiece c. What is the role of the condenser lens? a. Diaphragm Answer: e . Stage d. what structure varies the diameter of the cone of light? a. Increase the magnification c. Control the aperture of light b. 14. 15. Base b.

What is the bottom of a microscope called? a. . The revolving nosepiece is also called a turret. thus. The slide rests on the stage. Base b. 16. The objective lens are attached to the nosepiece (or turret). The revolving nosepiece is also called a turret. Nosepiece c. its name). Base b. The revolving nosepiece is also called a turret. Nosepiece c.The base is the bottom of the microscope. The objective lens are attached to the nosepiece (or turret). Stage d. 17. thus. its name). The tube is the structure which connects the eyepiece to the objective lenses (it is shaped like a tube. The slide rests on the stage. Tube e. The tube is the structure which connects the eyepiece to the objective lenses (it is shaped like a tube. The diaphragm controls the diameter of the cone of light. its name). The diaphragm controls the diameter of the cone of light. The slide rests on the stage. Where do you place the slide when using a microscope? a. The diaphragm controls the diameter of the cone of light. thus. Tube e. Diaphragm Answer: a The base is the bottom of the microscope. Diaphragm Answer: c The base is the bottom of the microscope. The tube is the structure which connects the eyepiece to the objective lenses (it is shaped like a tube. Stage d. The objective lens are attached to the nosepiece (or turret).

A phase contrast microscope is useful for examining living cells. A transmission electron microscope does not use light. Dissection microscope e.18. The compound microscope is also referred to as a "light microscope" or "bright field microscope". Transmission electron microscope Answer: c A simple microscope has only one lens. Phase contrast microscope d. but rather a beam of electrons. What is another name for the bright field microscope? a. Phase contrast microscope d. Dissection microscope e. Things appear three dimensional with a dissection microscope. A compound microscope utilizes two lenses: an ocular lens and an objective lens. Simple microscope b. A dissection microscope uses low power magnification. The compound microscope is also referred to as a "light microscope" or "bright field microscope". . 19. because the specimen does not need to be stained. A transmission electron microscope does not use light. A phase contrast microscope is useful for examining living cells. A compound microscope utilizes two lenses: an ocular lens and an objective lens. A dissection microscope uses low power magnification. Compound microscope c. because the specimen does not need to be stained. Simple microscope b. Things appear three dimensional with a dissection microscope. Transmission electron microscope Answer: b A simple microscope has only one lens. but rather a beam of electrons. Which microscope would be particularly useful for looking at living cells? a. Compound microscope c.

Things appear three dimensional with a dissection microscope. A transmission electron microscope does not use light. Compound microscope c. Transmission electron microscope Answer: a A simple microscope has only one lens. Phase contrast microscope d. A phase contrast microscope is useful for examining living cells. because the specimen does not need to be stained. The compound microscope is also referred to as a "light microscope" or "bright field microscope". . Simple microscope b. Dissection microscope e.20. A dissection microscope uses low power magnification. A compound microscope utilizes two lenses: an ocular lens and an objective lens. but rather a beam of electrons. Which type of microscope has only one lens? a.