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mClavGR2 PhotoconvertibleReporter Fusion Vectors
F
or Research Use Only. Not for Diagnostic or Therapeutic Use.
Purchase does not include or carry any right to resell or transfer this product either as a stand-alone product or as a component of another product. Any use of this product other than the permitted use without the express written authorization of Allele Biotech is strictly prohibited
Box 1 | Product List
Fluorescent Protein Vectors
Vector NameCat. Number
Page
pmClavGR2-CTABP-FP-CLAVC2pmClavGR2-NTABP-FP-CLAVN3
I
MPORTANT NOTICE TO PURCHASER:
These products may be subjects of pending U.S. and foreign patents. Allele Biotech grants academic/government research institutions a worldwide, non-exclusive, royalty-free, limited license to use this product for non-commercial life science research use only. Such license
specically prohibits the right to sell or otherwise
transfer this product or its derivatives to a third party.
Industry For-Proft Institutions:
Institutions that wish to use this product are required to obtain a sub-licensing agreement from Allele Biotech. For more information please visit Fluorescent Protein Sublicense, or contact us via phone or email.
A
llele’s photoconvertible proteins allow researchers to track the changes in localization in living cells by changing from green to red. These two fusion vectors are
monomeric photoconvertible uorescent expression vec
-tors with multiple cloning sites for fusions to the C and N termini of mClavGR2. Compared with previously available photoconvertible FPs, mClavGR2 has improved photosta-bility of the red state under confocal illumination condi-tions, 3644 over mEOS2’s 2700 and Dendra2’s 2420
[1]
. Most notable among other advantages of mClavGR2 is its monomeric structure, its highly optimized and rapid folding
efciency, and its superior photoconversion efciency due
to the high pKa of the green state.
[1]
Hoi H, Shaner NC, Davidson MW, Cairo CW, Wang J, Campbell RE: A monomeric photo-
convertible uorescent protein for imaging of dynamic protein localization. J Mol Biol. 2010 Sep 3;401(5):776-91.
587 ATCC
GCTAGC
TCTAGACT
GGATCC
TC
GAATTCGGTACC
TC
AAGCTTGCGGCCGCC
TCGAGNheI XbaI BamHI EcoRI KpnI HindIII NotI647 GCCACC
ATG
GTGAGCAAGGGCGAGGAGACCATCATGAGCGTGATCAAGCCTGACATGAAG707 ATCAAGCTGCGCATGGAGGGCAACGTGAACGGCCACGCCTTCGTGATCGAGGGCGAGGGC>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>1247 CACTTCGTGGACCACCGCATCGAGATCCTGAGCCACGACAAGGACTACAACAAGGTGAAG1307 CTGTACGAGCACGCCGTGGCCCACAGCGGCCTGCCCGGCATGGACGAGCTGTACAAG
TAA
CMV Promoter ........ 1-589Multiple Cloning Sites ........ 591-646mClavGR2 ........ 659-1366poly(A) Signal ........ 1397-1623 AmpicillinResistance Gene ........ 2210-3070pUCOrigin ........ 3225-3844Sequencing Primer: CMV-F and mClavGR2-R (ABP-FP-CLVRV)
Multiple Cloning Sites
pmClavGR2-CT (ABP-FP-CLAVC)
607 GCCACC
ATG
GTGAGCAAGGGCGAGGAGACCATCATGAGCGTGATCAAGCCTGACATGAAG667 ATCAAGCTGCGCATGGAGGGCAACGTGAACGGCCACGCCTTCGTGATCGAGGGCGAGGGC>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>1267 CTGTACGAGCACGCCGTGGCCCACAGCGGCCTGCCCGGCATGGACGAGCTGTACAAGTCC1327 GGACTCAGATCTCGAGTCGGATCCTCGAATTCGGTACCCTCTAGACAAGCTTGCGGCCGCBglII XhoI BamHI EcoRI KpnI XbaI HindIII NotI1387
TAA
CMV Promoter ........ 1-589mClavGR2 ........ 613-1323Multiple Cloning Sites ........ 1324-1386poly(A) Signal ........ 1428-1654 AmpicillinResistance Gene ........ 2241-3101pUCOrigin ........ 3256-3875Sequencing Primer: mClavGR2-F (ABP-FP-CLVFW)
Multiple Cloning Sites
pmClavGR2-NT (ABP-FP-CLAVN)
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