Fluorescent Dyes For Flow Cytometry & Microscopy

(IR) DyLight 800 Abs: 777 Emi: 794 App: F, CM Alexa Fluor 660 Abs: 663 Emi: 690 App: F, CM SL: Red-Diode Alexa Fluor 594 Abs: 590 Emi: 617 App: CM SL: Orange-He-Ne DyLight 649 Abs: 654 Emi: 673 App: F, CM SL: Krypton-Argon

This poster is a guide to some of the most useful uorochromes available for ow cytometry and immunouorescence/confocal microscopy today. The main chart separates single dyes according to laser excitation wavelengths and the luminous rings represent their actual uorescence color. Two of the laser lines shown can be used to excite tandem conjugates (the rst excited uorophore provides the energy to activate the second dye). This is particularly useful where, for example, you only have an Argon-Ion laser available but still want to gain access to uorophores with higher excitation and emission wavelengths. Tandem dyes are presented separately in the tables below. Although all the information has been checked for accuracy we highly recommend you always refer to actual product datasheets before starting your experiments. These dyes are environmentally sensitive and may produce different results in different buffers. Precise instrument settings, choice of lters and uorescence detection channels will also inuence outcomes. Please refer to your instrument handbooks for guidance.
Alexa Fluor 647 Abs: 650 Emi: 665 App: F, CM SL: Krypton-Argon

Tandem Conjugates See Table 1

FITC Abs: 490 Emi: 525 App: F, CM SL: Argon-Ion

TRITC Abs: 596 Emi: 570 App: CM SL: Krypton-Argon

APC Abs: 650 Emi: 661 App: F SL: Krypton-Argon

Alexa Fluor 555 Abs: 555 Emi: 565 App: CM SL: Krypton-Argon Tandem Conjugates See Table 2 Alexa Fluor 405 Abs: 401 Emi: 421 App: F, CM SL: Blue Diode R-Phycoerythrin Abs: 490,565 Emi: 578 App: F, CM SL: Argon-Ion

Pacic Blue Abs: 410 Emi: 455 App: F, CM SL: Violet Diode DyLight 405 Abs: 400 Emi: 420 App: F, CM SL: Blue Diode

Texas Red Abs: 596 Emi: 615 App: CM SL: Yellow-He-Ne

Alexa Fluor 488 Abs: 495 Emi: 519 App: F, CM SL: Argon-Ion

DyLight 549 Abs: 562 Emi: 576 App: F, CM SL: Green-He-Ne

Note: Human vision is in the range from 380 nm (blue) to 790 nm (red). Therefore, special equipment is required to visualize the far-red uorescent dyes. Abbreviations: Abs = Maximal Absorbance (nm), APC = Allophycocyanin, Appl = Application, CM = Confocal Microscopy, Emi = Maximal Emission (nm), F = Flow Cytometry, FITC = Fluoresceinisothiocyanate, IR = Infrared, PE = R-Phycoerythrin, PerCP = Peridinin-chlorophyll-protein complex, SL = Suggested Laser, TRITC = Tertramethylrhodamine

DyLight 680 Abs: 692 Emi: 712 App: F, CM SL: Red-Diode Cy3 Abs: 548 Emi: 570 App: CM SL: Green-He-Ne

Alexa Fluor 430 Abs: 433 Emi: 541 App: F, CM SL: Blue Diode PerCP Abs: 490 Emi: 675 App: F SL: Argon-Ion

DyLight 488 Abs: 493 Emi: 518 App: F, CM SL: Argon-Ion

Tandem Conjugates
DyLight 594 Abs: 593 Emi: 618 App: F, CM SL: Orange-He-Ne

Table 1 488nm
DYE PE-Texas Red PE-Alexa Fluor 610 PE-Cy5 PE-Alexa Fluor 647 PerCP-Cy5.5

ABSORBANCE 496,546 496,546 496,546 496,546 496,546 496,546 496,546 496,546 496,546 496,546

EMISSION 615 627 627 627 695 695 702 723 779 785

APPLICATION F F F F F F F F F F

LASER Argon-Ion Argon-Ion Argon-Ion Argon-Ion Argon-Ion Argon-Ion Argon-Ion Argon-Ion Argon-Ion Argon-Ion

FLUORESCENCE COLOR

Alexa Fluor 680 Abs: 679 Emi: 702 App: F, CM SL: Red-Diode

Alexa Fluor 546 Abs: 556 Emi: 573 App: CM SL: Green-He-Ne

DyLight 633 Abs: 638 Emi: 658 App: F, CM SL: Red-He-Ne

PE-Cy5.5 PE-Alexa Fluor 680 PE-Alexa Fluor 700 PE-Alexa Fluor 750 PE-Cy7

Near IR Near IR Near IR

Table 2 633,635,647nm
(Near IR) DyLight 750 Abs: 752 Emi: 778 App: F, CM Alexa Fluor 700 Abs: 702 Emi: 723 App: F, CM SL: Red-Diode (Near IR) Alexa Fluor 633 Abs: 632 Emi: 647 App: F, CM SL: Red-He-Ne

DYE APC-Cy5.5 APC-Alexa Fluor 750 APC-Cy7

ABSORBANCE 650 650 650

EMISSION 695 779 785

APPLICATION F F F

LASER Krypton-Argon Krypton-Argon Krypton-Argon

FLUORESCENCE COLOR

Near IR Near IR

Your rst choice for antibodies!

www.abdserotec.com

LITFLUORPOST.MAR.2009.1 MorphoSys UK Ltd 2009. All Rights Reserved. Creative Concept by Misha Rahman, Ph D. Alexa Fluor and Pacic Blue are Registered Trademarks of Molecular Probes Inc, USA DyLight is a Trademark of Thermo Fisher Scientic and its Subsidiaries CyDye is a Registered Trademark of GE Healthcare