Loi ni dau TCVN 8125 : 2009 hean toan tung dung vdi ISO 20483 : 2008; TCVN 8125 : 2009 do Ban kj thuat tiéu chudn quéc gia TCVN/TC/F13 Phuong phap phan tich va lay mau bién soan, Téng eye Tiéu chudn Bo lvéng Chat lveng dé nghi, B6 Khoa hoc va Cdng nghé dng béNg céc va dau dé — Xac dinh ham Iweng nite va tinh ham lugng protein thé — Phuong phap Kjeldahl Cereals and pulses — Determination of the nitrogen conten! and calculation of the crude protein cantent — Kjeldahl method CANH BAO = Vide ap dung tiéu chudn nay 6 thé lién quan dén cac vat ligu, thiét bj vi cdc thao tac gdy nguy hidm. Tigu chudin nay khéng thé dura ra duc hét tit cd cac vdn dé an toan lidn quan dén vigc si dyng chung. Nguéi si dung tiéu chudn nay phai ty thiét lp cdc thao tac an toan thich hyp va xac dinh kha nang ap dung céc gid han qui dinh trréc khi si dung tiéu chudn. 1 Pham vi ap dung Tiéu chuan nay quy dinh phyong phap xAc dinh ham lwong nite bang phuong phdp Kjeldahl va tinh ham long protein thé trong nga céc, dau d& va cac san phdm cla chung. Phwong phap nay khéng phan biét dude gitra nite’ protein va nite phi protein. Néu can phai xae dinh ham lweng nite phi protein thi phai ap dung phuong phap thich hop khac. CHU THICH Trang eas treéng hop cy thé, phurong phap nay khang thé thu duoc toan bé nite trong nitrat va ndrit, 2 Tai ligu vin dan Cac tai ligu vién dan sau rat can thiét cho viée ap dung tiéu chudn nay. Béi vdi cac tai liéu vién din ghi nam cing bé thi 4p dung phién ban duc néu. Bdi voi cac tai liéu vin dan khang ghi nam ebng bé thi ap dung phién ban mdi nht, bao gdm cd cac sia di, bd sung (néu cd). TCVN 4846 (ISO 6540), Ngd — Phuong phdp xde dinh ham lvgeng 4m (Ng6 bat va ngé hat). ISO 712, Cereals and cereal products — Determination of mofsture content - Routine reference method {Ng@ céc va san phdm ngO cde - Xée dinh dO dm (Phuong phap chudn théng dung).5.9 Axit boric, dung dich, o(H3BQ3) = 40 g/l hodc bat ky nang 46 nao khac theo hudng din st dyng thidt 61 5.10 Chat chi th} mau Bd sung cac thé tich Dung dich A (5.10.1) va Dung dich B (5.10.2) theo huéng on str dung thidt bj (vi du: 5 phan thé tich Dung dich A va 1 phan thé tich Dung dich B) ‘CHU THIGH 1 Ca thé sit dyng dung dich axit boric d& chudn bj sn co chia chat chi thi mau (5.9 + 6.10) ‘CHU THICH 2 Ty I@ Dung dich A va Dung dich B cé thé dugs didu chinh phy thude wan thidt bj G6 thé tién hanh chudn d6 bang dién thé st dung dién cye pH, cdn dug kiém tra hang ngay. 5.10.1 Dung djch A Xanh wrornborest (C2Hy4BrOsS): 200 mg. Etanol (C;HyOH), 95 % thé tich: mét lugng dU cho 100 mi dung dich. §.10.2 Dung dich B £18 metyl (CysH;sNaO2): 200 mg. Etanal (C2HsQH), 95 % phan thé tich: mét leng 4 cho 100 mi dung dich. 6.11 Natri hydroxit, dung dich (NaOH), 33 % khdi Iveng node 40 % khéi Iueng co ham lurgng nite nhd hon hodc bang 0,001 %. Cing 0d thé si dung natri hydroxit ky thuat Khi ham lueng nite nhd hon hoade bang 0,001 %. 5.12 Axit sulfuric, dung dich chudn, c(H,50,) = 0,05 mobil Nén st dung H2S0, thay che HCI vi H,SO, khéng tao bot khi trong cac dng néi 5.13 Amoni sulfat, dung dich chudin, o(NH,}, SO, = 0,05 moll. Cach khac, 6 thé str dung mudi (NH,)2Fe(SO.)2.6H,0. $.14 Ba bot, dang hat, d4 dugc niva trong axit clohydric va dugc nung 5.15 Sacaroza (tuy chon} khéng cha nite, 6 Thiét bi, dung cu 6.1 May nghién co hoc6.2 Sang, cé co 16 0,8 mm. 6.3 Can phan tich, cé thé can chinh xac dén 0,001 g. 6.4 Thidt bj phan hoy, chung cdt va chudn a6 Can phai chac chan sy phan bé déng daéu nhiét 46 cda thiét bj phan huy. Banh gia tinh ding déu cla nhiét d bang phép th voi mat trong hai chat chudn (5.6 hede §.7) va tinh 6 thu hdi dat duge. Cae thidt bi chung cat cing duge kiém tra xac nhan bang cach chung c4t mét lugng 44 biAt cua mudi amoni [vi du: 10 ml dung dich mudi amoni sulfat (5.13}] va kiém tra xem 4 thu hdi ¢¢ lén hon hode bang 99,8 % hay khang. 7 Liy mau Mau giri G&n phong thu nghiém phi la mu dai dién. Mau khong bi hu héng hode thay di trang sud qua trinh van chuyén hodc bao quan. Phuong phap l4y m4u khéng qui dinh trong tigu chudn nay. Nén idy mau theo ISO 6644 va TCVN 5451 (ISO 13690) & Chudn bi mau thir Néu can, nghién m&u sao cho toan b6 mau lot qua [6 sang 0,8 mm. Bdi véi cae hat, khdi lveng hat cén phai nghién it nhat lé 200 g. Tran déu mau da nghién. 9 Xa dinh a6 dm Xac dinh 66 4m (w,) cla mau tht? tie mét lwong mu 4 due chudn bi theo Didu 8. Tién hanh xac dinh bang phueng phap tht pha hop ddi véi ting san pham (vi dy ISO 712 adi vei ng& cfc va san phdm ng cdc, TCVN 4846 (ISO 6540) adi vai ngd, hode bang phuvong phap mé td trang [10] adi vai dau 4a) 10 Cach tién hanh 10.4 Yéu cdu chung Néu can, dé kiém tra cac-yéu cau vé gidi han lp lai (12.2) thi tian hanh hai phép xac dinh riéng ©é theo 10.2 dén 10.5.10.2 Phan méu thor Can mot luvgng mau thi da chudn bj theo Biéu 8, chinh xac dén 0,001 9, tuy thude vao ham [wong niter gia dinh sao cho phan mau thi chira tir 0,005 g dén 0,2 g nite va tdt nhat fa lon hon 0,02 g 10.3 Xac dinh 10.3.1 Phan huy GANH BAO — Cac thao tac sau phai thyc hién trong ti thang gid tét, ta hut khdi chiu durye axit sulfuric. Chuyén phan mau thw (10.2) vao binh phan huy, sau 4d thém: - 10g kal sulfat (5.1); ~ 030g dang (Il) sulfat ngam nam phan tl nude (5.2); ~ 0,30 titan oxit (5.3) (cd thé sir dung chdt xc tac & dang vién tong Ung vai thanh phan qui dinh}, va - 20-mi axit sulfuric (5.4). Cé thé diéu chinh Ivong axit sulfuric phy thuge vao thiét bi, mhung chi sau khi chdc chdn rang phép do nay can cé dé thu hdi 99,5 % dai vei axetanilit va 99,0 % d6i vei tryptophan. Trén ky, dé dam bdo rdng phan mau thy edt hoan todn. Bat binh trén thiét bj phan huy d4 due gia nhiét rude dén (420 + 10) °C. Sau it nhdt 2 h phan hug tinh tir thei diém nhiét 6 cla thiét bi dat (420 + 10) °C, lAy ra va dé ngubi. CHU THIGH Nén bd sung da bot (5.14) lam chét didu chin s6i va chat chéng tao bot nhu sap parafin (5.5) Théi gian phan huy tdi thidu duge kiém tra bang chat chudn nhung rdt khé @@ dat 68 thu hdl (xem 10.5) Theo khuyén edo cia nha san xuat thiét bi, khi sy bay hoi kéo dai s& lam tht thoat nite 40.3.2 Chieng cdt Can théin thém 50 m/ nue vao binh 4a nguéi va dé ngudi han. Chuyén vao binh hing 59 ml axit boric (5.9) va it nhAt 10 gigt chi thi mau (5 10}, quan sat mau hoac str dung dau dé quang hoc. Thém mét tweng du 5 ml dung dich natri hydroxit (5.11) cdn dé trung hoa Iveng axit sulfuric dé si dung. Sau dé tién hanh chung edt.Tuy thudc vao thiét bj, ma Iugng thude thir dugre stk dyng ed thé khac nhau. 10.3.3 Chuan a6 Tién hdnh chudn 46 bang dung dich axit sulfuric (5.12), thyc hién lién tuc trong sudt qua trinh chung cAt hoac khi két thc qua trinh chung cét trén toan bd dich cat. Xac dinh diém két thie qua trinh chung cat bing so mau hodc si’ dung ddu dé quang hoc hoac phan tich dién thé véi hé théng do pH 10.4 Phép ther trang Tién hanh phép thir trang vei cac thude thir str dung trong 10.3.1 dén 10.3.3 nhung khéng od mau ther (10.2) CHU THIGH Geé thé thay mau thir bang + g sacaroza (6.15). 10.5 Phép thé v@i chét chudn (Phép tht kidm tra) Say khé chat chudn duec st dung & nhiét a6 tir 60 °C dén 80 °C trong chan khang, veri sw cé mat cla phospho pentoxit (5.8). Tién hanh phép thir kiém tra trén phan mau thi tdi thiéu 0,15 g bing cach xac dinh ham Iueng nite eda tryptophan (5.7) va/hodc cila axetanilit (5.6) CHU THICH C4 thé thém 4 g saccaroza (6.15) vae chat chudn 4 thu héi nite tlr axetanilit it nhét phai la 99,5 % va a6 thu hdi nite ter tryptaphan It nhat 1A 99,0 % 41 Bidu thj két qua 11.1 Ham lureng nite Ham lveng nite, wy, bidu thi phan khdi lung chat khd, tInh bang phan tram (%), thea céng thire sau: wy = (ic MolxT x0,074x100 100 _ 140.7 (—Vo) NT Tm” OOS ma OO trang da: Vp la thé tich cua dung dich axit sulfuric (5.12) c&n cho phép the trang, tinh bang mililit (ml); ¥, la thé tich eda dung dich axit sulfuric (6.12) c&n cho phan mau tht, tinh bang millit (mil); 0,014 14 lveng nite twong duong voi viéc ste dyng 1 ml dung dich axit sulfuric 0,5 mol, tinh bang gam (a);T la néng a6 dong lugng cia dung dich axit sulfuric st dung dé chudn dé: m la khdi lurgng etia phan mau ther, tinh bang gam (g); wy la dé Am, duoc xde dinh theo Biéu 9. Biéu thi kat qua d&n hai chi sé thap phan. 14.2 Ham lueyng protein tha Tinh ham iwgng protein thé cla san phadm khé bang cach nhan ham ureng nite (141.1) thu dug & thoi diém xac dinh vai hé sé chuyén aéi phi hop cho loai san phdm ngi cdc hod dau a va vide sir dung chung Biéu thi kat qua dén mat chiy s4 thap phan. CHU THIGH Mot s6né sé chuyén ddi duoc sir dung cho nga cdc duye néu trong Phy luc C. Cas loai khan thuéng sir dung hd 64 6,25 12 Béchym 12.1. Phép the fién phong ther nghiém Cac chi tiét cla phép tht lién phdng tht nghiém vé dé chum cla phuong phap dues dua ra trong Phy \We A. Cac gid tr thu duge tle phép thi lién phdng thi nghiém nay cé thé khéng 4p dung cho cae dai néng 6 va cdc chat nén ngoai cdc dai ning 43 va chat nén da néu, 42.2 Dé lap lai Chénh léch tuyét 44i gidra cdc két qua ella hai phép th déc lap, don lé thu duoc khi sir dung cung phuong phap trén vat liéu tho gidng hét nhau trong cling mét phéng thi nghiém, do mét ngudi thy hién, si dung cling thiét bi, thire hién trang mét khodng thai gian ngan, khong qua 5 % cac truréng hep vuert qua gidi han lap lai rduge tinh theo cong thirc sau r= (0,0063 x wp) x 2,8 trong do wp la ham Iugng protein thé cia mu, biéu thi bang phan tram khéi lweng san phdm khd (xem Bang B.1). D6i voi cdc san phdm cé ham Ivong protein thé tiv 7 % dén 80 %. xem Bang A.1 va Hinh At. 12.3 6 tai ip Chénh léch tuyét d6i gita cdc két qua cla hai phép thir don Ié, thu dure khi str dung cing phuong phap trén vat liéu ther giéng hét nhau trong cae phong thi nghiém khac nhau, do nhing ngudi khacnhau thye hién, st: dung cac thiét bi khac nhau, khéng qua 5 % cdc trvéng hop vuet qua gidi han tai lap R due tinh theo cong thirc sau: R= (0,014 x wp) x 2,8 (vem Bang B.1). Hdi vai cae san pham co ham lung protein thé tir 7% dén 60 %, xem Bang A1 va Hinh A1, 12.4 Saisé téihan 12.4.1. So sanh phép do cia hai nhém trong mét phong thir nghiém Sai sé téi han (CDr) gitra hai gia tri trung binh, méi gia trj thu duge tir hai két qua ther du@i cac diéu kién lap lai, bang: CDr = 1,98 x s, = 1,98 x (0,0063 x We) = 0.01247 x Wp (xem Bang B,1) 12.4.2, So sanh phép do cua hai nhém trong hai phéng thir nghiém Sai sé 164 han (CDR) gitra hai gia tri trung binh, mi gia tr) thu dures tir hai két qua thd do hai phong thi nghiém khac nhau thy hién duéi cac didu kién lap lai, bang: i oS Te CDR = 2,8,/s’n -0,5.5% = 2,8,(0,014 x wp}? 0,5 x (0,0083 x wp)? = 0,03716 x we (xem Bang 8.1). 413 Bao cdo thir nghiém Bao céo thir nghiém phai ghi r6: a) moi théng tin cdn thiét 44 nhan biét day ati vé mu ther; b) phurong phap lAy mau da str dung, néu biét; c) phuong phap thir a4 sir dung, vién dan tiéu chudn nay: a) hé sé chuyén déi 4a str dung (xem Chi thich trang 11.2); e) moi chi tidt thao tac khang duge quy dinh trong tigu chudn nay hode nhiing didu duge col la tuy + cing nhw bat ky sy oé nao 66 thé anh hudng dén ket qua thi f} két qué thir thu duoc, hodc néu théa man yéu céu vé 46 [4p lai thi néu két qua cudi cing thu duge.Phy luc A (Tham khao) Két qua clia cdc phép ther lian phong thir nghiém 6 lap lai, 66 tai lap va sai s6 téi han cla phueng phap dure thiét ap bd hai phép thir lién phéng thir aghiém luan phién thea cac y&u cau clia phan 2, phan 3 va phn 6 cla TCVN 6910 (ISO 5725). Muri phdng thir nghiém tham gia vao phép tht? nay. Mudi bdn sdn phdm va bén chat chudn due dung ab phan tich. Cac két qué dua ra trong Bang A.1. Bang A.1 — Két qua théng ké cua cac phép thir lién phang the nghiém Théng sé Mau" 1 2 Sill Sea: 5 6 1 | ahs T f 86 lugng céc phdng thi nghiém cén lai sau khi dd loai try ngoai |é 2 # ip ag 10 19 ie Harn lugng protein trung binh (wy x 5,7), tink | \ theo % chétkhd, we 7,03 | 694 | 9.02 | 11,88 | 13,90 | 15.54 | 18,19 Bd lech chudn lap lai, s. O11 604 0,07 0,15 0,05 O07 O47 5 - | 1 He sé bién thién (a6 ch chudn dtrung bin, % 166 | 045 | O76 | 126 | 0643 | 046 | 1,05 ta | fan | GiGi han lap lai, r(= 2.8 xs] 031 | 011 | 020 | o42 | O17 | 0,20 | o48 | { aa | | 86 lech chuan tai ap, Se O19 | 014 008 O28 | 016 | O14 | O34 Me sé bidn thién (d lech chudn Rfrung "tinn), % | 2,70 | 187 | 089 | 236 | 1,15 | 090 ° 2,10 | — Gidi han tai lap, R (= 2.8 x se} 063 048 | 022 0,78 0,45 0,39 0,05 oe —l | * Gac mau 6 la: 1 = Bot my théng thuong 1; 2 = Ngé: 3 = Lua mach; 4= Lua my théng theréng: 5 = Bot | my théng thuéng 3; 6 = Lua my cleng: 7 = Bot my thueng 2.Bang A.1 (két thac) Mau | Thong sé : ee : a a 10 " az | 43 | 44 | 10 10 10 10 10 a sau khi da loai tror ngaai |e 54 long cdc phéng thi nghiém con jai | ‘0 | | I | Ham tug rotein trung binh (4, % 5,7}, tinh | y ‘ | | veg a (oS, 2191 | 22.80 | 31.57 | 6133 | 62,19 | 7346 Tagg thea % chat kha, Wp | Bo lech chu ae a, aoo | ai7 | 010 | oas | o27 | 057 | o19 | He sé bién thién (a0 Kech chudn rftrung aai | 075 ) a2 | 139 | 043 | ore | a4 inh), % | Gibi han iap lai, r= 28x) a26 | aaa | o2a | 2,38 | 0,76 | 1,80 | 088 6 lech chudn tai lap. 5p 029 | 046 | 048 | tar | 079 | 1.02 | 08s He s6 bidn thién (40 lech chudn Rftrung 1,32 2.02 146 2.23 1,27 1,28 1,04 bin), % Git han tai lap, R (= 2.8 x ss) |} oat | 128 | 128 | aaa | 221 | 2ae | 2,32 " Cac mau dé lad: 8 = Bau 2; 9 = Bau 1; 10 = Bau hat ngoai déng rung; 11 = Gluten bot my 1; 12 = Gluten bOtmy 2, 13=Glutenngé1; 14 = Gluten ngé2. pe © 5 10 1 me a an as MOS A) BS 7H TS BOM CHU DAN X Ham luveng protein, % Y 80 isch chudn, % Hinh 4.1 —Méi quan hé gitra dé l@ch chuan lap lai va 46 léch chuan tai lap va gia trj trung binh cua ham lugng proteinHinh A.1 cho thay d6 l&ch chuan lap lai va 40 lech chuan lap tai lap phy thuéc vao gia tri pratein tim thay, do vay cac gid tri nay khong phai la hang sé. Vide thiét lap méi quan hé phy thuc gila cac gia tr) vé 44 chum (dé lp lai hodc dé tai lap) va mirc trung binh cla protein tang 44i vei mot vai loai o4 lién quan, Tir sai 86 nhé quan sat dupe gilra cac mi quan hé dan dén chap nhan phwong trinh di qua didm géc toa do: a) phueng trinh duéng hdi quy déiveis, 5, = 0,0063 x We b) hésé xdc dinh R? = 0.4646 ¢) phuong trinh duéng hdi quy déivei se = $e = 0.014. x we d) hé sé xae dinh R*=0,7849Phu luc B (Tham khao) Sai sé toi han va 4p dung thie té c4c giéi han lap lai va tai lap cho cac ham lirgyng protein khac nhau 8.1 So s4nh cac phép do cda hai nhém trong mét phang the nghiém Sai sé téi han (CDr)" gitra hai gid tr| rung binh thu dugc tir hai két qua thir dudi cae diéu kién vé a6 lap lai bang: CDOr= 2,8, eats yen, A, trong dé §; la d6 léch chuan lap lai: nyvan, 1886 két qua thi tuong Gng vai mdi gid tri trung binh, Néu cd hai 1, v4 n; déu bang 2, thi phuremg trinh nit gon 1d B.2 So s4nh céc phép do cia hai nhém trong hai phéng thir nghiém Sai sé tei han (CDR) gitra hai gid tri trung binh thu dure tir hai két qua thir cla hai phong thi nghiém khac nhau durdi cdc diéu kién vé 46 lp lai bing: os a CDR =2.8 |s*e-s?,|1-—--__ \ Lez telly trong dé: 5 la a6 léch chun 1p lai: 5p la 6 léch chudn tai lp; mvan2 1a 36 két qua thir tong Ung voi mai gia tri trung binh. Néu ca hai n, van, déu bang 2, thi phuong trinh rit gon la: CDR = 2,8,/s*n -0,55875 "Sai s& 141 han IA sai sé gida nai gi8 bi tung birch thu dugc tir hat két qua thir trong cling dé lp lai, xem TCVN 8910-6 (150 5725-6).Bang B.1 - Ap dung thy té cila gidi han lap lai va gigi han tai lap d6i vei cdc ham Iuegng protein khac nhau Ham twomg | Delech | Gidingn | BB Igch : Gigi han | Sais tei nee (CD) gia bal gi protein chudin lap fai lépiai | chudn tai lap tai lap Se MUO d Jeb ele | | (Ww 5,7) : R | ‘Trong 1 phong | Trong 2 phadng % i | 7 thirnghiem | thérnghigm 10 Sra O17 | O14 “0.30 suas 037 ie #8 Po,08 0,25 a2 [058 L 30; 18 | 20 0.12 0,34 0.28 078 0.24 | 07s 28 015 ~ 0,42 035 0.98 0,30 0,93 x 018 a) a4z [418 0,36 112 cn oss |) (O4p 1.37 Tesigega tat a 36” (ORs 67 0.56 157 «| CB 7) ao [as 0.27 oe 0,63 1,76 053 166 CO 50 | 030 0.84 070 198 059 tar Essent (mae 0,82 0,77 216 0,85 2,05 “60 ass | 1,01 0.84 2.35 “Ort lig toaa 7 0,8 7,09 oa | (288 O77 23 «| 70 042 4,48 098 Bre 0,83 261 76 0.45 1,26 10 | 24 0,89 2,80 80 0.48 | 4,34 iz | "314 0.86 2.99 Cho phép: Phép thi 1) + | Phép thie2] = Trung binh 1 (phép tht 1+ phép thy 22 Phép thi 5) + Phép thé | = Trung binh 2 (phép thi 5 + phép thi 6/2 Phép thu 9} + [Phép thy 10} = | Trung binh 3 (phép thir 9 + phép ther 10)/2 VEDU: Gidi han lap lai duge ap dung gidra hode gia Gidi han tai lap duge ap dung giva hode gia Sai sd ti han (CD) duge dp dung gida hodc gia phép thik 1 — phép tha 2 phép thir 5 = phép tha 6 phep thir 4 — php tho 6 phép thar 2 phép thir & trung binh phep thy 1 - phép thir 2 trung binh phép thir 1 ~ pheép thi 3Phu luc C (Thar kha) Cac hé sé chuyén déi ham lurgng nite sang ham luegng protein sin pam le ee | Lua my thueng = 57 | Loa my ceng 5,7 San phdm lua my nghién : | 6,7 node 6,25 | Lea my dung lam this an | 6.25 i Liamach k ae 625 | ¥en mach z 87 nese 6.25 | Lia mach gen 57 Ngai ed¢ jai (Triticale) 6,25 Ng ie : : 6,25 | Blau db 625 |Thu myc tai ligu tham khao [A] 180 1871, Agricultural foad products - General directions for the determination of nitrogen by the Kjeldak! method. [2] ISO 3188, Starches and derived products — Determination of nitragen content by the Kjetdaht method - Titrimetric method. [3] TCVN 6910-2:2001 (ISO §725-2:1994), D6 chinh xde (d6 déng va d6 chum) cla phuong phap do va kéf qua do — Phan 2: Phuong phap co ban dé xac dinh dé tap Jai va dé tai lap cda pinrong php do tiéu chudn. [4] TCVN 6910-32001 (ISO 5725-31984), 86 chinh xac (dé ding va dé chum) ctia phuong phap do va két qud do — Phar 3: Cae thurre do trung gian dé chum cia phuong phap do tiéu chudn. [5] TCVN 6910-6 (ISO 5715-6), 86 chinh xde (46 dung vé dé chum) eda phuong phép do va két qué do— Phan 6: Sir dung cdc gia tri dé chinh xéc trong thie té, [8] TCVN 4328 (ISO 5983-1) Thitc dn chan nudi — Xac dinh ham fugng nite va tinh ham [ugg protein &khé. Phuong phap Kjeldahi. [7l 150 6644, Flowing cereals and milled cereal products — Automatic sampling by mechanical means. [8] TCVN 5451 (ISO 13690), Ngd ede, dau dé va sdn pham nghién — Lay mau tte khdi hang tinh. [9] NF V 03-050, Produits agricales alimentaires ~ Directives générales pour Je dosage de /azote selon fa méthode de Kjeldahl. (10) BIPEA, Conseil méthodologiques pour le dosage de /eau dans /es grains et les graines — LU68 E 8405 - Détermination de la teneur en eau — Proiéagineux (Fiche n° 4). [11] European Brewery Convention, Analytica EBC, 1984. [12] Nitrogen-ammonia-protein Modified Kjeldahl method Titanium oxide and copper sulfate catatyst. Official Methods and Recommended Practices of the AOCS, (ed. D.E Firestone). AOCS Official Method Ba Ai 4-91, AOCS Press, Champaign iL, 1997 713) TKACUK, R. Nitregen-to-protein conversion factors for cereals and oilseed meals. Cereal Cham., 46(4), 1969, pp. 419-423 [14] ICC Standard 105/2, Determination of crude protein in cereals and cereal products for food and feed