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Allele Biotech-Introducing Cost Effectiveness to Research
T
he Stealth Express Luciferase PCR template for In vitro tran-scription allows for the large-scale synthesis of mRNA encoding Lucif-erase gene. With the mRNA product, exogenous Luc expression can be achieved when transfected into target cells. This new gene delivery technol-
ogy is faster and more efcient than
conventional virus-based approaches.
10 μg of template is provide, enough
to make hundreds of micrograms of Luciferase encoding mRNA. Within each template, the coding sequence
for a protein is anked by 5’ and 3’ un
-translated regions designed to maxi-
mize the translational efciency and
cytoplasmic stability of the mRNA. The template drives incorporation of 120-nt polyA tails into RNA tran-scripts, eliminating the need to enzy-matically polyadenylate IVT products before use.
Features
Template contains the T7 phage polymerase promoter allowing the widely available T7 polymerase to be used.
 Inherently non-mutagenic PCR template for footprint-free gene expression
 Reliable expression levels
 Eliminates safety concerns and bio-containment issues associated with virus
Luciferase PCR Template
in vitro transcription template for large-scale synthesis of mRNAencoding Luciferase.
Box 1 | Product Info
ContentQty
Cat#: ABP-RP-SELUC
Luciferase linear DNA template ready to use directly in IVT reactions.
10 μg
Storage
-20
°
C
F
or Research Use Only. Not for Diagnostic or Therapeutic Use.
Purchase does not include or carry any right to resell or transfer this prod-uct either as a stand-alone product or as a component of another product.  Any use of this product other than the permitted use without the express written authorization of Allele Biotech is strictly prohibited
Box 2 | Components Needed for IVT
 
ComponentCat. No.Description
Modied UTP
 ABP-PP-NTPMODU
rNTPs for In vitro transcription. Modied
Uracil is required to ameliorate immunological responses towards the mRNA product.
Modied CTP
 ABP-PP-NTPMODC
rNTPs for In vitro transcription. Modied Cy
-tosine is required to ameliorate immunological responses towards the mRNA product.
Standard ATP
 ABP-PP-NTPSTDArNTPs for in vitro transcription.
Standard GTP
 ABP-PP-NTPSTDGrNTPs for in vitro transcription.
T7 polymerase
 ABP-PP-T7POLRNA polymerase for In vitro Transcription
ARCA
 ABP-PP-NTPARCAAnti-Reverse Cap Analog; transcripts produced with ARCA have increased translation in com-parison with conventional cap analogs.

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