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9700/31
BIOLOGY
Advanced Practical Skills 1
May/June 2011
2 hours
[Turn over
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You are reminded that you have only one hour for each question in the practical examination.
You should:
Plan your use of the time to make sure that you finish all the work that you would like to
do.
You will gain marks for recording your results according to the instructions.
contents
hazard
concentration
/%
volume
/ cm3
amylase solution
irritant
10
starch solution
none
50
copper sulfate
solution
harmful
irritant
0.03
20
distilled water
none
100
iodine
iodine in potassium
iodide solution
irritant
50
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Fig. 1.1 shows how to make the first concentration of 0.003% copper sulfate solution.
For
Examiners
Use
9 cm3 of
distilled
water, W
1 cm3 of
copper sulfate
solution, C
10 cm3 of 0.03%
copper sulfate
solution, C
0.003%
copper sulfate
solution
...................
...................
Fig. 1.1
(a) (i)
Complete Fig. 1.1 to show how you will make two further concentrations of copper
sulfate solution.
[3]
Proceed as follows:
1.
Prepare the concentrations of copper sulfate solution as shown in Fig. 1.1 in the
containers provided. Use the syringe labelled For copper sulfate.
2.
Label test-tubes with the concentrations of copper sulfate solutions and label
another test-tube W.
3.
Wipe the tile clean with a damp paper towel and then dry the tile. Label the tile, as
shown in Fig. 1.2. The numbers indicate the sampling times in seconds.
drop of
iodine
15
30
45
60
75
90
105
120
135
150
165
180
Fig. 1.2
4.
Put one drop of iodine on the tile at each sampling time, as shown in Fig. 1.2.
5.
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6.
7.
Put 0.5 cm3 of E into the same test-tube. Mix and start timing.
8.
9.
After 15 seconds use the glass rod to transfer a drop of the mixture to the iodine
drop, labelled 15, on the tile.
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5
(ii)
For
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[5]
(iii)
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(iv)
(v)
Table 1.1 shows the results of an investigation into the effect of the concentration of copper
sulfate solution on a protein suspension. A protein suspension was mixed with different
concentrations of copper sulfate solution.
After a set time, the percentage absorbance of light was measured using a colorimeter.
Table 1.1
copper sulfate
concentration
/ mol dm3
103
trial
1
trial
2
trial
3
trial
4
trial
5
mean
25.0
100
99
100
99
100
100
12.5
97
95
80
97
94
96
5.5
78
81
79
82
80
80
3.5
84
59
58
58
62
1.5
11
10
(b) (i)
UCLES 2011
Draw a circle around each of the anomalous results and complete the table.
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[2]
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7
(ii)
For
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[4]
(iii)
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2
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Fig. 2.1
(a) Draw a large plan diagram of the part of the leaf indicated by the shaded area in
Fig. 2.1.
Label the xylem and an air space.
[5]
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(b) Make a large drawing of six cells from the part of the leaf indicated by the shaded area
in Fig. 2.2.
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The cells should be two adjacent (touching) cells from the epidermis and two adjacent
cells from each of the next two layers.
Fig. 2.2
Label one epidermal cell.
[5]
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Fig. 2.3 is a photomicrograph of a transverse section through a leaf of a different plant
species.
Fig. 2.3
(c) The actual length of line Y is 785 m.
Use this measurement to calculate the magnification of Fig. 2.3.
You may lose marks if you do not show your working or if you do not use appropriate
units.
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(d) Prepare the space below so that it is suitable for you to record the observable differences
between the specimens on J1 and in Fig. 2.3.
Record your observations in the space you have prepared.
[5]
[Total: 18]
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University of Cambridge International Examinations is part of the Cambridge Assessment Group. Cambridge Assessment is the brand name of University of
Cambridge Local Examinations Syndicate (UCLES), which is itself a department of the University of Cambridge.
UCLES 2011
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