3.

3 mb, 435 pages

methods in molecular medicine

vascular disease
molecular biology and gene therapy protocols

1 detection of mutations and dna polymorphisms in genes involved in cardiovascular

diseases by polymerase chain reaction�single-strand conformation polymorphism
analysis
2 analysis of genetic variants in cardiovascular risk genes by heteroduplex
analysis
3 radiation hybrid (rh) mapping of human smooth muscle-restricted genes
4 efficient extraction of rna from vascular tissue
5 preparation of cdna libraries from vascular cells
6 screening cdna libraries using partial probes to isolate full-length cdnas from
vascular cells
7 cloning full-length cdnas from vascular tissues and cells by rapid amplification
of cdna ends (race) and rt-pcr
9 the use of differential mrna display (ddrt-pcr) to identify genes differentially
expressed in normal and diseased vascular cells
10 identification of novel protein kinases in vascular cells
11 primer extension analysis to map transcription start sites of vascular genes
12 use of liposome-mediated dna transfection to determine promoter activity in
smooth muscle cells
13 nuclear run-on assay to study gene transcription in vascular cells
14 electromobility shift analysis (emsa) applied to the study of nf-kappa b
binding interactions in vascular smooth muscle cells
15 measurement of gene expression in the vascular wall by reverse transcription-
polymerase chain reaction (rt-pcr) analysis
16 northern blot analysis to quantify gene expression in vascular diseases
17 localization of gene expression in human atherosclerotic lesions by in situ
hybridization
18 rnase protection assays for quantitation of gene expression in vascular tissue
19 detection of apoptosis in atherosclerosis and restenosis by terminal dutp nick-
end labeling (tunel)
20 in vitro detection of apoptosis in isolated vascular cells
21 molecular assessment of the phenotypic changes associated with smooth muscle
cells using two-dimension electrophoresis and microsequence analysis
22 simple methods for preparing recombinant adenoviruses for high-efficiency
transduction of vascular cells
23 generation of recombinant adeno-associated viruses for delivery of genes into
vascular cells
24 hemagglutinating virus of japan liposome-mediated gene delivery to vascular
cells
25 high-efficiency and low-toxicity adenovirus-assisted endothelial transfection
26 gene transfer in vascular cells using an engineered na-h exchanger (nhe1) as a
selectable marker
27 use of retroviruses to express exogenous genes in vascular smooth muscle cells
28 embryonal stem (es) cell�derived macrophages a cellular system that facilitates
the genetic dissection of macrophage function
29 delivery of recombinant adenoviruses to human saphenous vein
30 an in vivo angiogenesis assay to study positive and negative regulators of
neovascularization
31 efficient liposome-mediated gene transfer to rabbit carotid arteries in vivo
32 gene deliver y to rabbit arteries using the collar model
33 delivery of antisense oligonucleotides to the vascular wall
34 local gene delivery of recombinant adenoviruses to the rat carotid artery in
vivo
download :

http://rapidshare.de/files/34021575/030-
vascular_disease_molecular_biology_and_gene_therapy_protocols.pdf