Biological safety: Blood, human tissue, select agents and biologically-derived toxins, OPIM, biological agents by hazard classification,

, recombinant DNA (rDNA) Bacteria (T.B., Legionella), viruses (HIV, HBV), fungi (Candida Albicans), parasites (Malaria), rickettsiae (Rocky Mt. Spotted Fever), prions (CJD) A combo of lab practices and techniques, safety equipment, and lab facilities to reduce the risk The levels are designated in ascending order by degree of protection Level 1: suitable for work, not known to cause disease in healthy human adults o Level 2: agents that are associated with human disease, something has to happen: some kind of exposure, incident Level 3: exotic agents that have potential for high aerosol transmission, disease may have serious or lethal consequences Level 4: complete containment, agents with no cure, no therapy, viral agents, 7 rules of bio-safety: 1. Do not mouth-pipette 2. Manipulate infectious fluids carefully 3. Minimize contact with sharp objects: go into impermeable container 4. Use protective clothing and gloves 5. Wash hands after lab activities (30 secs) 6. Decontaminate work surfaces: prior to working with biological material, after any spill or viable material, when research is complete and all waste has been properly disposed Decontamination: should be accomplished by using 5.25% sodium hypochlorite diluted 1:10 with water: use a quarter cup of bleach 7. No eating/drinking in the lab BBP: microorganisms present in human blood Why the standard exists: to limit workplace esposures, to educate employees about safe work practices, to ensure training is provided Blood to blood contact or any other bodily fluid visibly contaminated with blood Ingestion, inhalation, injection, eyes, mouth, hose, non-intact skin, accidental puncture from contaminated sharps/needles 8. Hf Signs, labels, color: 1. Fluorescent orange, red, or orange-red, available from EH&S Biosafety cabinets: class II type a: filtered, not ducted, removes particulates, not vapors, sash height important HBV vaccination Annual refresher training

Biological spills: 1. Minor spill: alert people, close doors, wear gloves and lab coat, use forceps to pick up broken glass, discard into red sharps container, pour from outside in, allow 20 min contact time, dispose in biohazard waste container, re-spray disinfectant and wipe spill area with disinfectant 2. Major spill: Waste disposal: Autoclavable trays: sterilizer, steam, pressure Trays= only orange bags NO SOLIDS IN ORANGE BAGS Never autoclave solvents hkj

corrosives: chemicals with a low or high pH exposure risk can be reduced by: substituting for less toxic/ hazardous materials using as little material as possible handling inside a fume hood MSDS: must be readily available to you at all times Labeling and marking system

SEM COLLECTS HAZARDOUS WASTE Chemical name, building , room #, phone #, quanitity of chemical, Fax or call All buildings have fire alarms Strobe flashers for the hearing impaired PASS: pull, aim, squeeze, sweep