Molecular Biology Topic: DNA & DNA replication

1. 2. 3. 4. 5. 6. 7. 8. 10. Eukaryotic mRNA differs from prokaryotic mRNA by 1) Poly A tail 1) Fungal pathogens 1) 5 3 Exonuclease 1) Sense 1) 2-deoxy ribose Host for the bacteriophage is 1) Phage particle 1) 40 - 200 1) nutrient uptake 1) Accumulation of toxic metabolites 11. 12. 13. 14. 1) 2 1) E.coli 1) FISH 1) Can be done with any biological material 15. 16. 17. 1) Poly U at 3 terminus Chargoff rule is 1) A+T = G+C 1) Increases with temperature 18. 1) Promoter 2) A/C = G/T 2) Decreases with temperature 2) Terminator 3) A x G = C x T 3) is independent of the temperature 3) Ori 4) A/C = 1 4) it depends on the pressure Viscosity of the DNA solution 2) Bacteria 2) 400 2) Transcription 2) Depletion of nutrients 3) Fungi 3) 1000 - 2000 3) Translation 3) Presence of antibiotics 4) Yeast 4) 50 000 1 lakh 4) Cell wall biosynthesis 4) All of the above The number of replicons in a typical mammalian cell is Which one of the following is false, with regard to the killing of bacteria by antibiotic The growth rate of E.coli decrease in vitro culture due to 2) Methyl GTP cap at 5 end 2) Viral pathogens 2) 5 3 polymerase 2) Antisense 2) 3-deoxy ribose 3) Presence of both Nematodes 3) 3 5 Exonuclease 3) both 3) ribose 4) absence of both Insect pests 4) All of the above 4) none 4) both 1 & 2 Bt cotton became popular because it confers resistance against Proof reading or editing function is Strand of DNA has homology in base sequence to mRNA The sugar moiety present in tRNA is

Questions: 50

The number chromosome present in E.coli 2) 23 pairs 2) T4 phage 2) FPLC 2) It is based on coding sequence 2) Poly U at 5 terminus 3) 23 3) Retroviruses 3) ELISA 3) based on repetitive sequence 3) Poly A at 3 terminus 4) 1 4) Yeast 4) PCR 4) is useful in forensic science Replication of the following organism requires reverse transcriptase Several copies of the DNA segment can be obtained by the following process Of the following statements is false, with regard to DNA fingerprinting

Common feature found in most of the eukaryotic mRNA molecule is 4) Poly A at 5 terminus

The replication of DNA starts at 4) none of the above

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19. 20. 21. 22. 23. Sugar found in E.coli DNA is 1) deoxy glucose 1) Watson & Crick 1)P32 1) DNA 1) -amamitin & penicillin 24. 25. 26. 27. 1) uracil 1) mRNA A riboside is 1) Ribose + PO4+ base The Replication of DNA is 1) Conservative & continuous 28. 1) 100 strands of half heavy and 100 light 29. 30. 31. 32. 33. 34. 35. 36. The Tm of DNA increases if 1) G + C = A + T 1) Half of the original 1) hydrogen bond 1) Vanderwaal forces 1) 280 nm 1) Khorana 1) Watson crick turns 1) Denaturation of DNA 2) G + C > A + T 2) remains the same 2) ionic bond 2) -glycosidic bonds 2) 250 nm 2) Watson & Crick 2) Twisting number 2) Renaturation of DNA 3) A + T/2 = G + C 3) double 3) - glycosidic bond 3) ionic interactions 3) 260 nm 3) kornberg 3) Linking number 3) Super coiling 4) A + T > G + C 4) decreases 4) phospho-diester 4) Hydrogen bonds 4) 340 nm 4) Hershey & Chase 4) Writhing number 4) glycosylation The amount of the DNA in the cell just above to undergo cytokinesis is Bases are attached to the Sugar molecule in DNA by The bonds that hold two DNA strands together in double helix are The maximum absorbance of Nucleic acids is In vitro DNA synthesis for the first time done by The topoisomers are the DNA molecules that differ in Topoisomerases are associated with 2) Semi conservative & continuous 2) 100 heavy and 100 light 3) Semi conservative & discontinuous 3) 200 strands having half heavy 4) Conservative & discontinuous 2) Ribose + base 3) Ribose + PO4 4) Base + PO4 2) deoxy fructose 2) Hershey and Chase 2) S35 2) mRNA 2) Acridine and cycloheximide 2) uridine 2) DNA 3) Ribose 3) Rosalind Franklin 3) C14 3) tRNA 3) Ethidium & chloremphenicol 3) uric acid 3) rRNA 4) deoxy ribose 4) Mathew meselson 4) N15 4) rRNA 4) Tetracyclin and cycloheximide X-ray crystallogram of DNA was taken by Isotope used in Meselson and Stahls experiment is Nucleolus is the site of synthesis of Eukaryotic mitochondrial transcription and translation are inhibited by

Which of the following is nucleoside 4) Urea 4) tRNA The adaptor molecule of translation is

If 50 heavy DNA (N15) are allowed to replicate twice in the medium N14 the resultant DNA is 4) 50 heavy DNA strands

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37. 38. 39. 40. 41. 42. 43. 44. 45. 46. The DNA polymerase mainly concerned DNA synthesis is 1) DNA Pol - I 1) DNA Pol - I 1) Hydrogen Bonds 1) it increases Tm 1) Watson crick turns 1) DNA Polymerase 1) Arthur Kornberg 1) 5- AATGGCTAGC - 3 1) DNA pol I 1) 5 3 Exonuclease & 5 3 polymerase 47. 48. 49. 50. Primase is coded by 1) dna A 1) ATP 1) RNA pol -I 1) Pulse labelling 2) dna B 2) NAD+ 2) DNA pol - III 2) Pulse chase 3) dna E 3) NADP+ 3) DNA pol - I 3) Electrophoresis 4) dna G 4) GTP 4) DNA helicase 4) Chromatography DNA Ligase of E.coli requires Nick translation and strand displacement are carried out by Existence of Okazaki fragments was invented by Reiji Okazaki with the experiment 2) DNA Pol - II 2) DNA Pol - II 2) Phospho-diester 2) It decrease Tm 2) Twisting number 2) RNA polymerase 2) Baltimore and Temin 2) 5- TTCGGCTACG - 3 2) DNA pol - II 2) 3 5 Endonuclease & 5 3 polymerase 3) DNA pol - III 3) DNA Pol - III 3) Both of these 3) No effect 3) Linking number 3) Primase 3) Crick 3) 5- TTACCAATTCG-3 3) DNA pol - III 3) 5 3 Exonuclease & 3 5 Exonuclease 4) All of these 4) All of these 4) glycosidic bonds 4) it protects DNA 4) Writhing number 4) Topoisomerase 4) J.D. Watson 4) 3- TTACCAATTCG-5 4) DNA ligase 4) 3 5 Exonuclease & 5 3 polymerase The enzyme mainly concerned with DNA Repair During denaturation of DNA which of the following bonds are not broken In rDNA technology experiments formamide is used because The topoisomers are the DNAs that vary in Okazaki fragments in the DNA synthesis are primed by Reverse transcriptase was discovered by If a strand of DNA has the following sequence 5- AATGGTTAAGC - 3 what is the sequence of opposite strand 5 3 Exonuclease activity is characteristic feature of Klenow fragment of DNA polymerase has following functions