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North Maharashtra University, Jalgaon.

Class:- F.Y.B.Sc. Subject: Microbiology With Effect from June, 2009

The board of studies in Life Sciences in its meeting held on 15.04.2009 resolved to accept the revised syllabus for F.Y.B.Sc. (Microbiology) as per guidelines of Academic Council and with references to UGC model curriculum. The courses codes and titles for the courses are as given below. Mb: Microbiology YSC: Y- Year, S Semester, C Course No.

Course Code
Mb 111 Mb 112 Mb 121 Mb 122

Title of the Course

Elementary Microbiology Fundamental Methods in Microbiology Growth & Ultra Structure of Bacteria Isolation, Characterization & control of Microorganism

Semester 1 1 2 2

Lectures Ext. 40 40 40 40 40 40 40 40

Marks Int. 10 10 10 10

Mb 103

Practical Course





North Maharashtra University, Jalgaon

Syllabus for F.Y.B.Sc. Microbiology (With effect from June 2009) Mb 111: Elementary Microbiology Unit I- Types of Microorganism (General characteristics and Significance of Following microorganisms) A) Bacteria B) Archaebacteria C) Algae D) Fungi E) Protozoa F) Viruses G) Actinomycetes Unit II-Scope of Microbiology a) Microbes in food and Dairy industries b) c) d) e) Industrial products Genetic engineering and biotechnology Environment Agriculture (12 L) (12 L)

f) Bioterrorism Unit III-History of Microbiology a) Discovery of Microbial world b) Spontaneous generation-controversy c) The golden age of microbiology i) ii) iii) iv) Fermentation and Pasteurization The Germ theory of Disease Vaccination Chemotherapy- synthesis drugs and Antibiotics (16 L)

Mb-121: Growth and Ultra structure of Bacteria. Unit IV Growth and Reproduction of Bacteria a) b) c) d) e) f) Concept of Growth Reproduction (binary fission, Budding, Fragmentation Formation of Conidiospores and Sporagiospores) Growth rate and generation time Mathematical expression of growth Growth curve of bacterial population and its practical Applications Quantitative measurement of bacterial growth (Total Cell count, viable countbiomass determination, significance of growth measurement) (22 L) (18 L)

Unit V-Morphology and Fine Structure of Bacteria a) Morphology of Bacteria i) Size and shape ii) Arrangements Ultra structure of Bacteria Structure, function and chemical composition of the Following i) Capsule ii) Flagella iii) iv) v) vi) Pili and Fimbriae Cell Wall (Gram positive &Gram negative) Cell or cytoplasmic membrane Mesosome

b) c)

vii) Cytoplasm, Nucleoid (bacterial chromosome) and ribosomes. viii) Cytoplasmic inclusion volutine granules, PHB granules, glycogen, carbohydrates, Magnetosome, Gas vacicals, chlorosome, sulphur granules. d) Spore and Cyst i) ii) Endospore and Exospores Germination and Sporulation of endospore

References: 1. Modi, H.A. Elmentary microbiology Vol .I,Akta Prakashan,Nadiyad. 2. Modi, H.A. Elmentary microbiology Vol.II,Akta Prakashan,Nadiyad. 3. Dubey, R.C.and maheshwari,A. Text Book of Microbiology,S.Chand Publications New Delhi. 4. Tortora,Funke & Case Microbiology-An Introduction ,8 Edn, Pearson Education, Delhi. 5. Pawar and Daginawala, General Microbiology Vol.II. Himalaya Publishing House,Mumbai. 6. Stainer, R.Y.Iingraham,J.L. Wheelis M.L.PainterR.K.(1995) General microbiology, 5 Edition,MacMillan Press Ltd.London. 7. Pelczar,m.j.Chan ECS,Krieg NR(1998)5 Edition,Tata McGraw Hill Publication Co.Ltd.New DELHI. 8. Salle, S.J.(1974).Fundamental Principals of Bacteriology, Tata McGraw Hill Publication Co.Ltd.New DELHI. 9. Frobisher M.Hinsdill,Crabtree and Goodherat(1974) Fundamentals of Microbiology, 9 Edition,WB Saunders Co.USA. Mb-112: Fundamental Methods in Microbiology Unit I -: Microscopy a) Definition b) Comparison of Bright field and Dark Field Microscope c) d) e) f) Lenses of cofound microscope Image formation in compound microscope. Numerical aperture and immersion oil Resolving power (12 L)

g) Magnification h) Aberration. Unit II: Biological Staining a) Concept of stains and Dyes b) Uses of staining c) Acidic and basic dyes d) Mordents. e) Simple and differential staining (Acid Fast and Gram Staining)

(12 L)

Unit III: Cultivation of Microbes a) Bacteria i) ii) Nutritional requirements of bacteria. Media Common ingredients -Construction of culture medium -Types of Media iii) iv) b) i) ii) Enrichment culture method. Cultivation of Anaerobic bacteria.

(16 L)

Viruses Cultivation of animal viruses (in living animals, Embryonated eggs and cell cultures.) Cultivation of bacteriophage in laboratory.

Mb-122: Isolation, characterization and Control of Microbes Unit IV: Pure culture technique and identification of Bacteria a) b) c) d) e) Isolation by plating method ( Streak plate, pour plate Spread plate and roll tube method) Isolation in liquid media Two membered cultures Cultural characteristics on solid and in liquid media. Biochemical Characteristics. (22 L) (16 L)

Unit V: Control of Microorganisms a) b) c)

Fundamentals of Control. Mode of Action of antimicrobial agents. Physical agents High Temperature, Low temperature, Desiccation, Osmotic PRESSURE, Radiation, Ultraviolet lights,X- rays, Gamma rays,Cathod rays, surface tension and interfacial tension, filtration. Chemical agents : Characteristics of an ideal antimicrobial chemical agent, Definition of Sterilization, Disinfectant, Antiseptic, Sanitizer, Germicidal, Bactericides.


Major groups of chemical antimicrobial agents with practical allocation and mode of action. 1. Phenol and phenolic compound. 2. Alcohol 3. Halogen 4. Heavy metals and their compounds 5. Dyes 6. 7. 8. 9. Detergents Quaternary ammonium compounds Aldehydes Gaseous sterilization Practical course: Basic Techniques in Microbiology 1. 2. 3. Use and care of compound Microscope. Introduction of laboratory Instruments. Water bath,Autoclave,Hot air oven,pH meter, Weighing balance Specrtophotometer, Centifuge Refrigerator. Introduction and use of common Laboratory Glass Wares: i) Test tube, culture tube, screw-capped tubes ii) iii) iv) v) Petri dish. Pipettes Pasture pipette Erlenmeyer flask

4. 5. 6. 7. 8. 9. 10. 11. 12.

vi) Numeric flask vii) Glass spreader Monochrome staining. Negative staining Gram staining Acid fast staining Hanging drop technique Preparation of culture medium-(a)nutrient broth and agar,(b)MacConkeys broth and agar Isolation of bacteria by streak plate technique Enumarition of bacteria by pour plate technique from soil sample (serial dilution) Enumerations of bacteria by serial dilution and spread plate technique from soil sample .

13. 14.

Study of commen colony characteristics of (a)E.coli,(b)Staph.aureus. Biochemical test for Identification of bacteria (a) Sugar,GlucoseLactose,Sucrose,Maltose (b)H2s test byT.S.I. and Lead acetate paper technique. IMViC Test. Effect of temperature on growth of bacteria. Effect of pH on growth of bacteria. Effect of heavy metal on growth of bacteria. Demonstration of permanent slides (Anabena, chlorell, mucor, yeast, penicillium, amoeba, paramecium). Growth curve of E.coli.

15. 16. 17. 18. 19. 20.

References: 1. Dubey R.C.and Maheshwari D.K. 2004 Practical Microbilogy, S.Chand and Co. Delhi. 2. Aneja K.R.(1996) Experiments in Microbiology, 3 Edition Wishwa Prakashan,New Delhi. 3. Deshmukh A.M. (1997) 1 Edition, Handbook of Media, Stain and reagents in Microbiology pama publications. 4. Goud R.S. AND Gupta G.D.Practical microbiology, Nirali prakashan, pune.