Académique Documents
Professionnel Documents
Culture Documents
of
Histology and
Embryology
Editor: Fu Wenyu, Zhang Shengming, Guan Yingjun, Yue Bingde,
Lv E, Yu Li
2006.2
PREFACE
2
The second part, A Practice Guide Book of Histology, is written for the use in the
laboratory classes to help to develop students manipulating skills, observing power,
and problem analyzing and solving ability. The third part, Review of Histology and
Embryology, is a collection of self-evaluation questions for students to use to check
the result of their study and find what they need to pay more attention to. Through
these efforts we hope to improve the quality of our education and increase the ability
of students to meet the challenge of revolution of science and technology.
(Zhang Shengming)
CONTENTS
3
Chapter 9 Circulatory System………………………………………………...…14
4
The teaching of theory
Emphasis: The general structure of the light microscope. The method to study
histology.
Difficulty: The method to study histology. The method of paraffin section stained
with hematoxylin and eosin (H-E).
Contents:
The method most commonly used is a paraffin section stained with hematoxylin and
eosin (H-E). H behaves as a basic stain and stains the nucleus purple-blue color; E is
an acid dye and stains the cytoplasm pink to red color. Tissue component that stain
readily with the basic dyes are called basophilic; those with an affinity for acid dyes
are termed acidophilic. Tissue components that do not readily stein with both basic
and acid dyes are called neutrophilic. Certain dyes stain tissue components a
different color from that of the dye solution. The color change in the dye is called
5
metachromasia. Some tissue structures can be visualized with silver impregnation in
Which silver ions are deposited on tissues and reduced to silver particles showing
brown to black color --argyrophilic.
1.Emphasis:
2.Contents of practice
(Zhang Shengming)
(2) Master the electron micrographs of microvilli, cilia and cell junctions.
Emphasis:
6
Contents:
(7)Glandular epithelium and glands: derive from covering ones; descend into
connective tissue; make up glands.
(8)Specific structures in epithelium: ①Free surface: Microvilli and cilia. ②Lateral
surface: Tight junction(zonula occludens), intermediate junction(zonula adherens),
desmosome(macula adherens), gap junction(communicating junction), junctional
complex. ③Basal surface: Basement membrane, plasma membrane infolding and
hemidesmosome.
1.Emphasis:
2.Contents of practice
7
(1)Slides
(2) Video: Showing various covering epithelia, the ultrastructure of microvilli, cilia
and cell junctions.
(3) Homework: Draw the LM structure of simple columnar epithelium.
(Zhang Shengming)
(1) Master the morphology of cells and fibers in loose connective tissue.
(2) Understand and be able to recognize dense connective tissue, adipose and reticular
tissues.
Emphasis: The morphology of cells and fibers in loose connective tissue, the
function of cells and fibers in loose connective tissue
Difficulty:The ground substance and the structure of molecular sieve
Contents:
(1)Loose connective tissue: Most common and widely distributed type. Major
component is ground substance with a few cells and fibers(areolar).Soft and flexible.
①Cells—7 types: Fibroblasts, Macrophages, Plasma cells, Mast cells, Fat cells,
Undifferentiated mesenchymal cells and Leukocytes. ②Fibers—3 types: Collagen
8
fibers, elastic fiber and reticular fiber. ③The ground substance: Major constituents are
1.Emphasis:
(1) master the morphology of cells and fibers in loose connective tissue.
(2)Understand and be able to recognize dense connective tissue, adipose and reticular
tissues.
2.Contents of practice
(1) Slides
②Plasma cells
(3)Homework: Draw the LM structures of the cells and fibers in loose connective
tissue.
(Zhang Shengming)
9
The teaching of theory
Contents:
(2)Bone: Specialized connective tissues with calcified matrix, thus firmer than
cartilage, and provides support, movement, protection and a storage site for calcium.
The teaching of practice
1.Emphasis:
2.Contents of practice
(1) Slides
②Elastic cartilage
10
④Cartilaginous ossification(finger of a human fetus)
(3) Homework: Draw the LM structures of the hyaline cartilage and bone.
(Zhang Shengming)
(2) Master the classification, structure and function of peripheral blood cells.
Emphasis:
The classification, structure and function of peripheral blood cells, the basic
conception of hematopoietic stem cell.
Difficulty: The development process and morphologic changes of hematopoietic cells
in hematopoiesis.
Contents:
(1) Blood :
The composition of blood: consisting of formed elements and the blood plasma.
11
Erythrocyte: the structure, metabolizable features and functions of red blood cells.
The amount and life-span of red blood cells in the normal peripheral blood. The
structural features of reticulocytes.
1. Emphasis
(2) Master the classification, structure and function of peripheral blood cells.
2. Contents of practice
(1) Slide
No.18
12
(3) Homework: Draw the light microscopic structures of erythrocytes, leukocytes and
blood platelets.
(Lv E)
(1) Master the light microscopic structure and function of skeletal muscle, cardiac
muscle and smooth muscle
(2) Master the ultrastructure of skeletal muscle and cardiac muscle
(3) Understand the molecular structure and contraction mechanism of skeletal muscle
Emphasis:
Contents:
(2) Skeletal muscle: the light microscopic structure of skeletal muscle; sarcomere,
myofibril, dark and light bands(or A band and I band), the ultrastructure of
skeletal muscle, myofilament, transverse(T) tubules, sarcoplasmic reticulum(SR,L
tubule), triad, the molecular structure and contraction mechanism of skeletal
muscle. The composition of skeletal mucsle: epimusium, permusium,
endomysium.
13
(3) Cardiac muscle: compare the light microscopic structure and the ultrastructure
between skeletal muscle and cardiac muscle. the ultrastructure of intercalated
discs.
(4) Smooth muscle: the light microscopic structure and the ultrastructure of smooth
muscle, the junctions of the adjacent cells.
The teaching of practice
1. Emphasis:
(1) Master the light microscopic structure and function of skeletal muscle, cardiac
muscle and smooth muscle.
(2) Master the ultrastructure of skeletal muscle and cardiac muscle.
2. Contents of practice
(1) Slides
(2) Video: The structure of skeletal muscle, cardiac muscle and smooth muscle.
(3) Homework: Draw the light microscopic structures of 3 types of muscular tissue.
(Lv E)
(1) Master general structure of nervous tissue, the structural and functional features of
neuron and neuroglial cell
14
(2) Master the light microscopic and ultrastructural features, function and
classification of synapse
(3) Master the classification and structure of nerve fibers
Emphasis:
(1) General structure of nervous tissue, the structural and functional features of
neuron and neuroglial cell.
(2) The light microscopic and ultrastructural features, function and classification of
synapse.
(3) The classification and structure of nerve fibers.
Contents:
(2) Neuron: cell body, the light microscopic structure and the ultrastructure of
dendrites and axons, the classification of neurons according to the number of
processes and the neurotransmitters released, the structural base of axonal
transport. the junctions of the adjacent neurons: the light microscopic and
ultrastructural features of synapse,
(3) The classification of neuroglial cells: Four types of neuroglial cells in the CNS:
the structural and functional features of astrocytes, oligodendrocytes, microglia
and ependymal cells. Two types of neuroglial cells in the PNS: the structural and
functional features of Schwann cells and capsular cells.
(4) Nerve fibers: the light microscopic structure of myelinated nerve fiber, Schwann
cell plasmalemma, the formation and ultrastructure of myelin sheath. The light
microscopic and ultrastructural features of unmyelinated nerve fibers.
15
The teaching of practice
1. Emphasis:
(1) Master general structure of nervous tissue, the structural and functional features of
neuron and neuroglial cell.
(2) Master the light microscopic structure and ultrastructural features, function and
classification of synapse.
(3) Master the classification and structure of nerve fibers.
2. Contents of practice
(1) Slides
① Neuron No.30
(Lv E)
(2) Understand the histological organization of the cerebral cortex ,cerebellar cortex
and spinal cord
(3) Understand the basic structure of peripheral nervous system.
(4) Understand the histological composition and function of the blood-brain barrier,
the nutrition supplied features of nervous system.
Contents
16
(1) CNS: the basic structure of CNS, the histological organization of the cerebral
cortex, cerebellar cortex and spinal cord.
(2) PNS: the basic structure of PNS, histological features of ganglia and nerve, the
classification of nerve endings, the histological features and function of tactile
corpuscle, lamellar corpuscle,muscle spindle, free nerve ending, motor end plates.
(4) The nutrition supplied features of nervous system. the histoligical composition
and function of the blood-brain barrier.
The teaching of practice
1. Emphasis:
(2) Understand the organizational structure of the cerebral cortex, cerebellar cortex
and spinal cord
2. Contents of practice
(1) Slides
② Cerebellum No.31
③ Cerebrum No.33
(3) Homework: Draw the light microscopic structure of cerebellum, and the
ultrastructure of synapse
(Lv E)
17
The teaching of theory
Contents:
(1) General structure of the wall of the heart and blood vessels.
(3) Artery: the structural features and functions of large artery, medium-sized artery
and small artery.
(4) Vein: the structural features of small vein, medium-sized vein and large vein.
(7) Lymphatic vessels: the structural features and function of lymphatic capillary. The
structural features of lymphatic vessel and lymphatic duct.
18
The teaching of practice
1. Emphasis:
2. Contents of practice
(1) Slides
① Heart No.39
(3) Homework: Draw the light microscopic structure of the wall of the medium-sized
artery and the heart.
(Lv E)
19
(2) Master the classification of lymphocyte and functions of all kind of lymphocytes.
(3) Master the structures and functions of central and peripheral lymphoid tissue.
(4) Master the general structure and functions of lymph node and spleen.
Emphasis: the structures and functions of lymph node and spleen. mononuclear
phagocyte system. Blood-thymus barrier.
Difficulty: the classification of lymphocyte and functions of all kind of lymphocytes,
mononuclear phagocyte system
Contents:
(5) Thymus: structure: capsule, cortex, medulla, blood-thymus barrier. the functions
of thymus
(6) Lymph nodes: interstitium; cortex, superfacial cortex, paracortical zone, cortical
lymphoid sinuses; medulla, medullary cords, medullary lymphoid sinuses. The
functions of lymph node. recirculation of lymphocytes
(7) Spleen: general structure; white pulp, periarterial lymphatic sheath, lymphoid
nodules; red pulp, splenic sinusoids; marginal zone. The functions of spleen
20
The teaching of practice
1. Emphasis:
2. Contents of practice
(1) Slides:
①Thymus No.43
③Spleen No.42
(3) Homework: Draw the light microscopic structures of lymph node and spleen.
(Fu Wenyu)
(3) Understand the locations, structures and functions of melanocyte and Langerhans
cell.
(4) Understand the structures and functions of Sebaceous gland and Sweat gland.
21
Contents:
(1) Skin: General structures of epidermis and dermis. Layers, structure, ultrastrucure
and keratinization of thick epidermis from base to top. The locations, structures and
functions of melanocyte and Langerhans cell. Layers of dermis, the characteristics of
papillary layer and reticular layer.
(2) Skin appendages: hair, sebaceous gland, sweat gland and nail
1.Emphasis
2. Contents of practice
(1) Slides:
(Fu Wenyu)
(1) Understand the general structures of the endocrine glands, the ultrastructural
characteristics nitrogen-containing hormone-secreting cells and steroid hormone-
secreting cells
22
(2) Master the histological structures and their secreting hormones of thyroid gland,
parathyroid gland and adrenal gland.
(3) Master the histological structures and their secreting hormones of pituitary gland.
The relationship between hypophysis and hypothalamus.
Emphasis: the structures and functions of thyroid gland, parathyroid gland, adrenal
gland and hypophysis, hypophyseal portal system, relationship between hypophysis
and hypothalamus.
Difficulty: the steps of thyroid hormone synthesis and secretion, relationship between
hypophysis and hypothalamus
Contents:
(2) Thyroid gland: general structure, structure and ultrastructure of thyroid follicles,
the processes of thyroid hormone synthesis and secretion, distribution, structure and
ultrastructure of parafollicular cells and the hormone they secreted.
(3) Parathyroid gland: general structure, structure of chief cells and the hormone they
secreted. structural characteristics of oxyphil cell.
(4)Adrenal gland: general structure. Cortex: structures of zona glomerulosa, zona
fasciculate and zona reticularis, hormones that they secreted. commom ultrastructural
features of cortical cells. Medulla: structure, ultrastructure and hormone they secreted
of medullary cells. Blood supply of adrenal gland.
(5) Relationship between hypothalamus and hypophysis: morphology, structure and
subsections of hypophysis. Adenohypophysis: structure and classification of
acidophil(somatotroph and mammotroph) in pars distalis, structure and classification
of basophil(thyroitroph, gonadotroph and corticotroph), structure of chromophobe
cell, structure and function of pars intermedia, structure of pars tuberalis, commom
ultrastructural features of endocrine cells of hypophysis. Neurohypophysis: structure
23
of pars nervosa, structures and hormones(antidiuretic hormone and oxytocin) it
secreted of Herring body. Relationship between hypothalamus and adenohypophysis,
hypophyseal portal system. Relationship between hypothalamus and
neurohypophysis.
The teaching of practice
1.Emphasis:
(1) Master the structures of thyroid gland, adrenal gland and hypophysis.
2. Contents of practice
(1) Slides:
③Hypophysis No.94
(2) Video: The structure of thyroid gland, adrenal gland and hypothysis
(3) Homework: Draw the light microscopic structures of thyroid gland, adrenal gland
and hypophysis.
(Fu Wenyu)
(1) Master the general structures of digestive tract and the structural characteristics
and functions of each organ.
24
(2) Understand the distribution, kinds and functions of enteroendocrine cells in
digestive tract.
Emphasis: the general structures of digestive tract, histological structures of the
esophagus, stomach and small intestine.
Difficulty: mucous-HCO3- barrier(stomach mucous barrier)
Contents:
(1) General structures of the digestive tract: Mucosa, epithelium, lamina propria,
muscularis mucosae. Submucosa, villi, plicae. Muscularis externa. Serosa or
adventitia, serosa, adventitia
1.Emphasis:
25
2. Contents of practice
(1) Slides:
①Esophagus No.50
②Stomach No.53
③Duodenum No.54
④Jejunum No.55
⑤Ileum No.56
⑥Colon No.58
⑦Appendix No.59
(2) Video: The structures of esophagus, stomach, small intestines, large intestine and
appendix.
Homework: Draw the light microscopic structures of esophagus, stomach and
duodenum.
(Fu Wenyu)
(1) Master the morphological characteristics of serous acini, mucous acini and mixed
acini.
(2) Master the structure and function of pancreas.
26
(3) Master the structure and function of liver, the structures of hepatic lobule and
portal area.
(4) Master the ultrastructural features and functions of the hepatocyte and sinusoid.
Emphasis: the morphological characteristics of serous acini, mucous acini and mixed
acini, the structure and function of pancreas, the structure and function of liver, the
structures of hepatic lobule and portal area, the ultrastructural features and functions
of the hepatocyte and sinusoid, the characteristics of blood supply of liver.
Difficulty: the ultrastructural features and functions of the hepatocyte and sinusoid.
Contents:
(1) General structures of the salivary glands, acini and duct system. serous acini,
mucous acini and mixed acini. Distinctive features of three pairs of salivary glands
(2) Pancreas gland
(3) Liver
General structure of liver, the light microscopic structures of hepatic lobule, central
veins, hepatic plates, the light microscopic structure, ultrastructural features and
functions of the hepatocyte, the ultrastructure of bile canaliculi, the light microscopic
structure and ultrastructural features of sinusoid, structure and function of Kupffer
27
cells, structures and functions of perisinusoidal space and fat-storing cells. Constitute
and structure of portal area. Characteristics of blood supply of liver. Passage of bile.
The teaching of practice
1. Emphasis:
(2) Understand the structure of parotid gland, submandibular gland, sublingual gland.
2. Contents of practice
(1) Slides:
①Pancreas No.66
④Parotid gland
⑤Submandibular gland
⑥Sublingual gland
(3) Homework: Draw the light microscopic structures of pancreas and liver.
(Fu Wenyu)
28
Chapter 15 Respiratory System
Emphasis: the structure of trachea, the structure of lung, the ultrastructure and
function of alveoli.
Difficulty: the ultrastructure and function of alveoli.
Contents:
(1) Nasal cavity: The parts of nasal cavity. The structures and functions of nasal
mucous menbrane in vestibular region, respiratory region and olfactory region.
(2) Trachea and bronchus: Structure of the trachea and bronchus. Mucous, submucosa
and adventitia. The light microscopic structure, ultrastructural feature and functions of
the epithelium, ciliated cell, goblet cell, basal cell brush cell and diffuse
neuroendocrine cell.
(3) Lung: General structure of the lung, pulmonary lobule, constitutes and structural
changes of pulmonary conductive portion. Constitutes and structures of pulmonary
respiratory portion, structural features of respiratory bronchiole, alveolar sac and
29
1. Emphasis:
2. Contents of practice
(1) Slides:
①Trachea No.71
②Lung No.74
(3) Homework: Draw the light microscopic structure of trachea and lung.
(Fu Wenyu)
(1)Master the distribution, the structure of light and electron microscope and the
functions of the nephron, collecting tubule and papillary duct.
(2) Master the composition and the structure of the juxtaglomerular complex.
(4)Understand the general structure of the renal calyx, renal pelvis ureter and urinary
bladder.
Emphasis: The structure of light and electron microscope and the functions of the
nephron and the composition and the structure of the juxtaglomerular complex.
Difficulty: Difference of the structure between the proximal convoluted tubule and
the distal convoluted tubule.
Contents:
30
(1) Kidney: General structure: the location and composition of the cortex, medulla,
renal pyramid, medullary, cortical labyrinth and renal column, the composition of the
uriniferous tubule. Nephron: The light and electron microscopic structures and
functions of the renal corpuscle. The structure and the function of the filtration barrier.
The structure of light and electron microscope and the functions of the renal tubule.
Juxtaglomerular complex: the composition and structure of the juxtaglomerular
complex. The function and structure of the juxtaglomerular cell, macula densa and
extraglomerular mesangial cell. Interstitial tissue: the function and structure of the
interstitial cell.
(2) The structures of the renal calyx, renal pelvis ureter and urinary bladder.
1. Emphasis
(2) Master the ultrastructure of the filtration barrier of the renal glomerulus.
2. Contents of practice
(1) Slides
①Kidney No. 74
(2) Video: The structures of kidney, injected renal blood vessels and urinary bladder.
(3) Homework: Draw the light microscopic structure of the renal cortex.
(Yue Bingde)
31
(1) Master the structure of the seminiferous tubule and the conception of the
spermatogenesis and spermiogenesis.
(2) Master the structure and the function of the interstitial cell and the conception of
blood-testis barrier.
(3) Know well the structure of the prostate.
(4) Understand the general structure of the testis and the structure of the epididymis
and ductus deferens.
Emphasis: The structure of the seminiferous tubule. the structure and the function of
the conception of blood-testis barrier.
Difficulty: The conception of the spermatogenesis and spermiogenesis.
Contents:
(1) Testis: General structure: the structure of the seminiferous tubule. The light and
electron microscopic structures and functions of the Sertoli cell. Spermatogenesis and
spermiogenesis. Blood-testis barrier and the light and electron microscopic structures
of the spermatozoon.
(2) Epididymis: the structure and the function of the ductulus efferentes and ductulus
epididymis.
(3) The structure of the ductus deferens.
(4) Prostate: the composition of the prostate. The structure of the acinus.
1. Emphasis
(2) Master the morphology of the spermatogenic cell, Sertoli cell, Leydig cell and
spermatozoa.
2. Contents of practice
(1) Slides
32
①Testis No. 79
②Prostate No. 83
(2) Video: The structures of the testis, epididymia, protate and spermatozoa.
(3) Homework: Draw the structures of the seminiferous tubule and the interstitial
cells.
(Yue Bingde)
(1) Master the structure of the ovary, the development and maturation of the ovarian
follicle.
(2) Master the formation, structure and function of the corpus luteum, the conception
of the ovulation.
(3) Master the structure of the uterus, master the relationship between the cyclic
changes of the uterine endometrium and the ovary.
(4) Know well the structure of the oviduct.
Emphasis: the development and maturation of the ovarian follicle, the relationship
between the cyclic changes of the uterine endometrium and the ovary.
Difficulty: the relationship between the cyclic changes of the uterine endometrium
and the ovary.
Contents:
(1) Ovary: General structure of the ovary, the development and maturation of the
ovarian follicle and the structure and function of the ovarian follicle, the formation,
structure and function of the corpus luteum. Ovulation.
33
(2) Oviduct: The structure and function of the oviduct.
(3) Uterus: The structure and function of the uterus,specially uterine endometrium.
The cyclic changes of the uterine endometrium.
(4) Vagina and mammary gland: The structure of the vagina and mammary gland.
1. Emphasis
(1) Master the structural features of the ovary and corpus luteum.
(2) Master the structural features of the uterus and histological changes in the
endometrium during a normal menstrual cycle.
2. Contents of practice
(1)Slides
①Ovary No. 83
(3) Homework: Draw the structures of the ovary cortex and uterine endometrium.
(Yue Bingde)
34
(2) Master three tunics (outer fibrous tunic, middle vascular tunic and inner retina)
enclosing the refracting media (aqueous humor, lens and vitreous body) of the
eyeball.
(4) Understand the bone and membranous labyrinth of the inner ear, containing
receptors for both hearing and position.
Emphasis: Three tunics of the eyeball: outer fibrous tunic (composed of cornea,
anterior1/6 and sclera, posterior5/6), middle vascular tunic (consisting from anterior
to posterior of iris, ciliary body and choroids) and inner retina (composed of 4 layers
of cells forming 10 layers of retina).
Difficulty: The bone and membranous labyrinth of the inner ear, containing receptors
for both hearing and position.
Contents:
(1) Eye:
Eyeball: The compositions, structures and functions of the fibrous tunic, vascular
tunic and retina. macula lutea and papilla of the optic nerve. The contents of eyeball.
(2) Ear: External ear: the structure of the external ear. Middle ear: the structure of the
middle ear. Inner ear: the bone and membranous labyrinth of the inner ear, containing
receptors for both hearing and position.
1. Emphasis
(2) Master the structure of the spiral organ in the inner ear.
2. Contents of practice
(1) Slides
35
①Eyeball No. 100
(Yue Bingde)
(2) Master the development of embryo in preembryonic period and the process of
implantation.
(3) Master the development of embryo in embryonic and fetal period.
(5) Master the structures and functions of fetal membrane and placenta .
Contents:
Gametogenesis in the male and female, sperm capacitation. Time, position, process
and significance of the fertilization.
(2) Development of embryo in preembryonic period
36
①Cleagvage and formation of blastocyst: process of cleagvage, formation of
of chorion.
②Development of notochord
③Differentiation of three germ discs: formation of neural plate, neural groove and
37
①Fetal membranes: formation and functions of chorion, amnion, yolk sac, allantois
(Guan Yingjun)
Contents:
Contents of practice
38
Samples, models and video: Development of the face and palate, Cleft lip, oblique
facial cleft, Cleft palate.
(Guan Yingjun)
Contents:
39
laryngotracheal groove, development of larynx, trachea and lung. tracheoesophageal
fistula,hyaline membrane disease.
Contents of practice
Samples, models and video: Development and malformations of digestive tract and
digestive glands and respiratory system.
(Guan Yingjun)
40
②Development of ureteric bud and metanephrogenic tissue; position changing of
metanephros.
kidney.
(2) Development of genital system
1. Emphasis
41
differentiation.
Difficulty:
Contents:
42
cordius、ventricle、atrium and sinus venosus); formation of bulboventricular loop.
1. Emphasis
2. Contents of practice
Samples, models and video: Fetal heart; development and malformation of the
cardiovascular system.
(Yu Li)
43
Difficulty: differentiation and migration of neural crest.
Contents:
(1) Development of central nervous system: development of spinal cord and brain;
development and evolution of brain vesicle; differentiation and migration of neural
tube.
1. Emphasis
2. Contents of practice
Samples, models and video: Development of the brain and its congenital
malformations.
(Yu Li)
Understand the development of the eye and ear and congenital malformations.
Contents:
44
(1) Development of the eye: optic cup; lens placode; origin of the part of eye.
(2) Development of the ear: development of the external ear、middle ear and inner ear.
1. Emphasis
Understand the development of the eye and ear and congenital malformations.
2. Contents of practice
Samples, models and video: Development of the eye and ear and their congenital
malformations.
(Yu Li)
45