Biology 2EE3 Multiple choice questions

practice midterm questions

October 2010

1. Barely ____________% of all microbial species can be cultured in the laboratory. a. 50 b. 25 c. 10 d. 1 e. 0.1 2. In which one of the following types of microscopy is the specimen shadowed with heavy metal? a. atomic force microscopy b. SEM c. TEM d. X-ray diffraction e. dark-field 3. The chromosome includes a number of extra twists called supercoils. All of the following are true of supercoiling except a. DNA doubles back and twists upon itself. b. In bacteria and eukaryotic cells, there is positive supercoiling. c. This results in compaction. d. It is generated by gyrase. e. It is maintained by DNA-binding proteins. 4. Light is harvested by protein complexes called __________. a. phycobilisomes b. carboxysomes c. gas vesicles d. storage granules e. magnetosomes 5. Hydrolysis of ATP with the release of pyrophosphate is the driving force of which of the following reactions? a. DNA synthesis b. RNA synthesis c. aminoacylation of tRNA d. all of the above e. none of the above 6. Which of the following typically will survive autoclaving under standard laboratory operating conditions? a. mesophile b. thermophile c. endospore former d. Mycobacterium tuberulosis e. None of the above.

Biology 2EE3

practice midterm questions

October 2010

7. Archaea resemble eukaryotes in all but which of the following? a. nuclear membrane b. DNA-packing proteins c. RNA polymerase d. ribosomal components e. DNA polymerase 8. Agarose gel electrophoresis separates DNA based on a. size. b. charge. c. sequence. d. all of the above. e. none of the above. 9. All of the following statements regarding phospholipids are true except a. They consist of glycerol with ester links to two fatty acids and a phosphoryl group. b. They impart rigidity to the membrane. c. All face each other tail-to-tail. d. The two layers in the bilayer are called leaflets. e. They give the membrane a consistent thickness. 10. E coli tolerates a pH range of 29, which is a what-fold difference in hydrogen ion concentration? a. 7-fold b. 100-fold c. 1,000-fold d. 10,000-fold e. 10,000,000-fold True/False questions 11. Antiseptics and antibiotics are both used orally to fight bacterial infections. 12. Hopanoids reinforce and stiffen membranes in bacteria. 13. The ABC transporter superfamily contains only efflux transport systems. 14. The membranes of mesophiles are composed mostly of saturated lipids that bend against each other and align poorly, making the membranes of mesophiles more fluid at lower temperatures. 15. Strict anaerobes do not use oxygen as a terminal electron acceptor, which is why they die in air. 16. DNA and RNA polymerases both require primers. 17. Plasmid or phage DNA itself may integrate into the bacterial genome. 18. Some pathogenic bacteria use proton-driven efflux pumps to expel antibiotics from the cell.