Tng hp cc loi enzym c ti PTN B mn

Numb er 1 2 3 4 5 6 7 8 9 10 11 12

Names Termamyl SC Spezyme Xtra Spezyme Alpha Mats L classic Liquozyme SC DS Optimash TBG Optimash VR Filtrase NL Optimash XL Viscozyme Cassava R Distillase L400 Distillase ASP

Producer s Novozyme s Genencor Genencor DSM Novozyme s Genencor Genencor DSM Genencor

Type of enzymes Alphaamylase s

Micro organisme Bacillus licheniformi s Bacillus licheniformi s Bacillus licheniformi s Bacillus licheniformi s Bacillus licheniformi s Talaromyce s emersonii Trichoderm a reesei Aspergillus aculeatus Aspergillus niger Bacillus licheniformi s and Trichoderm a reesei

Hot lc 240 KNU-S/g 14000 AAU/g 13,775 AAU/g

Optimal pH 5,5 to 6,0 5,0 to 6,7 5,7 to 5,8 5,7 to 7,5

Optimal temperat ure 85 to 900C 800C

Dosage 100 to 350 g/ton starch 0,2 to 0,4 kg/ MT DS 0,2 to 0,24 kg/MT DS 0,22 to 0,40 g/MT DS 0,1 to 0,15 kg/MT

Time

83 to 850C 85 to 900C 850C 75 to 850C 75 to 850C 70 to 800C 700C 50 to 650C <500C 40 to 500C 58 to 650C 58 to 650C

240 KNU-S/g 5625 u/g

5,0 to 6,0 4,5 to 6,0 5,0 to 6,0 3,7 to 4,5 4,5 to 5,0 4,0 to 5,5 3,5 to 6,5 4,0 to 5,0 4 to 4,5 4,0 to 4,5

Betaglucana ses

6200 IU/g 100 FBG/g 350 GAU/g 580 TGAU/g

0,05 to 0,1 kg/MT DS 0,1 to 0,3 kg/MT 0,06 to 0,08 % w/w 0,05 to 0,075 % w/w 0,48 to 0,84 kg/ton 0,8 to 3,2 kg/ ton of starch 1,0 to 2,5 kg/MT

Genencor Genencor

Glucoamylase s

13 14 15

Dextrozyme GA Amigase Mega L Stargen 001

Novozyme s DSM Genencor Glucoamylase Aspergillus niger Aspergillus kawachi, 456 GSHU/g

4,2 to 4,5 4,0 to 4,5 4,0 to 4,5

60 to 620C 55 to 600C 20 to 400C

Spezyme Xtra, Spezyme Alpha: Alpha Amylase Units (AAU). Enzyme activity is determined by the rate of starch hydrolysis, as reflected in the rate of decrease in iodine-staining capacity. One AAU unit of bacterial -amylase activity is the amount of enzyme required to hydrolyze 10 mg of starch per minute under specified conditions. Optimash TBG: one unit of -glucanase activity is defined as the quantity of enzyme which produces reducing sugars equivalent to 1 mole of dextrose per minute from barley -glucan under standard assay conditions pH 5.0 and 50C (122F). Optimash XL: One IU unit of activity liberates 1 mol of reducing sugars (expressed as glucose equivalents) in one minute under specific assay conditions (50C (122F), pH 4.8). Stargen 001: Granular Starch Hydrolyzing Units (GSHU), which is calculated from the level of enzyme activity on raw starch and glucoamylase activity on soluble dextrin under prescribed conditions. Enzyme activity is an indicator of the expected rate of starch hydrolysis. Stargen 002, Distillase ASP, Distillase L-400: One Glucoamylase Unit (GAU) is the amount of enzyme that will liberate one gram of reducing sugars calculated as glucose per hour from soluble starch substrate under the conditions of the assay. Fermgen: Spectrophotometric Acid Protease Units (SAPU). One SAPU is the amount of enzyme activity that liberates one micromole of tyrosine per minute from a casein substrate under conditions of the assay. Termamyl SC, Liquozyme SC DS: 1 KNU (Kilo Novo Unit) is defined as the amount of enzyme which hydrolyses 4870 mg (on a dry basis) of soluble Merck starch (Erg. B 6, lot number 6380528) per hour under standard conditions, pH 5.6, 37C, and Ca2+ concentration 0.0003 M (Novozymes A/S, 2001a). Viscozyme Cassava R: One Fungal beta-glucanase unit (FBG) is the amount of enzyme which according to the above outlined standard conditions, releases glucose or reducing carbohydrate with a reduction capacity equivalent to 1 mol glucose/min. ACCELLERASE 1000 : The activity of ACCELLERASE 1000 enzyme complex is expressed in carboxymethycellulose (CMC U) activity units. One CMC U unit of activity liberates 1 mol of reducing sugars (expressed as glucose equivalents) in one minute under specific assay conditions of 50C (122F) and pH 4.8.

Beta-glucosidase is reported in pNPG units. One pNPG unit denotes 1 mol of Nitrophenol liberated from paranitrophenyl-B-Dglucopyranoside in 10 minutes at 50C (122F) and pH 4.8