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BMS 1004 Lab Fall 2011 Text: Introduction to Cell and Molecular Biology: BMS1004, Publisher: Mcgraw-Hill, Edition:

: Custom Edition, ISBN-10: 0-390-66606-8

YOU MUST READ THE FOLLOWING BEFORE THE FIRST DAY OF CLASS: A. Assigned Reading ix-xiii and have the signed released form ready to hand in on the first day of class. B. Assigned Reading How to write a scientific Paper or Lab Report ,Exercise 1 and 2 (pp xv-18)

**YOU WILL BE TESTED ON YOUR KNOWLEDGE OF ASSIGNED READINGS DURING THE FIRST WEEK OF CLASS**

GRADING: Lecture: 60% Lab: 40% Quizzes - 40% Pre Lab Report/Post Lab Report 40% Practical 10% Technical 10% ATTENDANCE: Attendance to the lab are mandatory, any missed labs may be made up during the same week of the lab missed. Make up labs are at the discretion of the Professor and the Head TA. This is a PRIVILEGE and not a right. If permission is granted you must make an appointment with the HEAD TA to reserve a seat in the make-up lab. 2 missed labs will constitute a failing grade. 2 late nesses will constitute an absence.

HOW TO PREPARE FOR CLASS: 1. Read ahead and prepare a Pre-lab to be handed in before you start class. The Pre-labs will be graded.

2. You must wear a lab coat in class. In the event you forget your lab coat, one can be rented from the lab. You may only do this ONCE a semester. 3. You may NOT wear open toed or meshed shoes at any time. 4. DO NOT enter the Lab without a Teaching Assistant or a Professor being present. 5. DO NOT apply lip gloss, lip stick or any other lip protectors in lab. 6. DO NOT wear jewelry in class. 7. DO NOT use your cell phones or any other electronic media unless you have the consent of the Professor. 8. Adhere to the safety protocol found in your preface of your lab manual. Exercises: 1. The Microscope Exercise 3 Determining Total Magnification; Understanding stage manipulation; the Letter e and Understanding Depth of Field; the 3 strings. Preparing a wet mount and observing living organisms, Questions to be answered; Q7a-c. 2. Solutions, Acids and Bases: the pH scale Exercise 5 Questions 1a-1c, 2a-2j and 3a-3c (DO AT HOME AND HAND IN AT THE BEGINNING OF CLASS). Procedures 5.1, 5.2 and 5.3. Questions 6a-c and 7a-b. 3. Biologically Important Molecules, Part I: Carbohydrates Exercise 6 Procedures 6.1 and 6.2. Questions 2a-d and 3a-c. Biologically Important Molecules, Part II: Proteins and Lipids Exercise 6 Procedures 6.3, 6.4 and 6.5. Questions 5a-c, 6, 7 and 8a-8e. 4. Separating Organic Compounds: Column Chromatography and Paper Chromatography Exercise 7 and Exercise 13. Procedures 7.1 and 13.1. Exercise 7 Questions 2a-b. Exercise 13, Questions 1 and 2a-e. Exercise 13 will be posted on Blackboard. 5. Separating Organic Compounds: Gel Electrophoresis Exercise 7. Procedure 7.3. Pre Lab Questions will be posted on blackboard; (DO AT HOME AND HAND IN AT THE BEGINNING OF CLASS) 6. Diffusion, Osmosis and Tonicity: Membrane transports Exercise 9. Procedures 9.4 and 9.5. Questions 5a-d and 6a-b. 7. Spectrophotometry: Identifying Solutes and Determination of Concentrations Exercise 8. Determining the Standard Curve, Procedure 8.2. Determining the concentration of a unknown, Procedure 8.3. Hand in the graph of the standard curve and the concentration of the unknown.

8. Enzymes I - Affects by Temperature Exercise 11. Revised Procedure will be posted on blackboard (DO PRE LAB ACCORDINGLY) Questions 1a-j. Enzymes I - Affects by Inhibitors Exercise 11. Revised Procedure will be posted on blackboard (DO PRE LAB ACCORDINGLY) 9. Enzymes II - Affects by pH Exercise 11. Revised Procedure will be posted on blackboard (DO PRE LAB ACCORDINGLY) Questions 2a-2h. Enzymes II Affects of Concentration Exercise 11. Revised Procedure will be posted on blackboard (DO PRE LAB ACCORDINGLY) 10.Cellular Respiration Exercise 12. Procedure 12.1, Questions 3a-3i. Cell Cycle: Mitosis Exercise 14. Questions 2a-b. Procedure 14.3 and 14.4 Questions 2a-b, 3a-b, 4a-b. 11.Genetics I Exercise 17, pp169-182. Observing Corn and Codominance of Blood Types. Procedure 17.2, Procedure 17.3, and Procedure 17.4. Questions 10a-c. Genetics II Exercise 17, pp175-177. Human Traits. Table 17.1, Determine the self genotype, phenotype and the possible gametes you can produce. Table 17.3, Phenotypes and Genotypes of Human Traits 12. Final - Practical