24h(N1)

CBD 0 5 10 15 20 µg/ml DMSO 0 5

mean
%
เทียบกับ
DMSO

1 2 3 4 5 6 7 8 9
A 0.044 0.043 0.044 0.042 0.044 0.044 0.043 0.042 0.045
B 0.044
C 0.043
D 0.045
E 0.051 0.045 0.045 0.045 0.044 0.044 0.043 0.044 0.046
F 0.952 0.731 0.601 0.906 0.894 0.044
G 0.51 0.568 0.443 0.901 0.883 0.044
H 0.689 0.574 0.589 0.96 0.89 0.045

1 2 3 4 5 6 7 8 9
A 0.047 0.043 0.044 0.043 0.044 0.044 0.043 0.042 0.043
B 0.044
C 0.044
D 0.043
E 0.045 0.044 0.044 0.044 0.045 0.044 0.043 0.044 0.045
F 0.902 0.767 0.826 0.827 0.787 0.044
G 0.838 0.777 0.717 0.75 0.77 0.046
H 0.904 0.806 0.788 0.796 0.789 0.045

1 2 3 4 5 6 7 8 9
A 0.044 0.042 0.044 0.042 0.044 0.044 0.043 0.042 0.044
B 0.044
C 0.043
D 0.044
E 0.045 0.044 0.045 0.045 0.045 0.043 0.044 0.044 0.045
F 1.457 1.467 0.989 0.752 0.131 0.043
G 1.452 1.415 1.009 0.676 0.085 0.044
H 1.498 1.534 1.004 0.771 0.079 0.044

1 2 3 4 5 6 7 8 9
A 0.044 0.042 0.043 0.045 0.044 0.043 0.042 0.043 0.043
B 0.048
C 0.044
D 0.043
E 0.047 0.044 0.044 0.044 0.044 0.043 0.043 0.044 0.044
F 1.38 1.255 0.914 0.568 0.072 0.045
G 1.419 1.3 0.85 0.493 0.075 0.045
H 1.493 1.229 1.09 0.495 0.082 0.047

1 2 3 4 5 6 7 8 9
A 0.044 0.042 0.044 0.043 0.044 0.047 0.042 0.042 0.044
B 0.814 0.873 0.639 0.557 0.107 0.051 0.835 0.817 0.693
C 0.824 0.735 0.742 0.236 0.052 0.052 0.817 0.772 0.67
D 0.926 0.741 0.863 0.137 0.052 0.052 0.756 0.768 0.818
E 0.047 0.047 0.045 0.045 0.044 0.044 0.044 0.045 0.044
F 0.899 0.781 0.763 0.666 0.258 0.054 0.778 0.713 0.792
G 0.919 0.76 0.739 0.679 0.857 0.055 0.748 0.769 0.68
H 1.02 0.76 0.77 0.729 0.393 0.055 0.784 0.747 0.802

1 2 3 4 5 6 7 8 9
A 0.044 0.042 0.044 0.043 0.044 0.044 0.042 0.043 0.044
B 0.859 0.782 0.448 0.333 0.053 0.057 0.834 0.783 0.751
C 0.809 0.736 0.436 0.135 0.051 0.052 0.843 0.745 0.701
D 0.81 0.677 0.368 0.153 0.143 0.052 0.822 0.739 0.794
E 0.044 0.044 0.044 0.045 0.045 0.044 0.043 0.045 0.045
F 0.833 0.789 0.749 0.898 0.763 0.532 0.83 0.709 0.644
G 0.82 0.765 0.73 0.729 0.189 0.055 0.768 0.673 0.68
H 0.853 0.833 0.755 0.678 0.371 0.066 0.807 0.743 0.707

1 2 3 4 5 6 7 8 9
A 0.044 0.042 0.042 0.041 0.045 0.044 0.043 0.042 0.043
B 0.038 0.041 0.04 0.042 0.042 0.04
C 0.041 0.044 0.046 0.043 0.042 0.036
D 0.043 0.045 0.044 0.041 0.044 0.043
E 0.046 0.046 0.045 0.045 0.044 0.045 0.044 0.046 0.046
F 0.722 0.724 0.684
G 0.678 0.645 0.647
H 0.704 0.709 0.706

1 2 3 4 5 6 7 8 9
A 0.044 0.042 0.044 0.041 0.044 0.044 0.043 0.043 0.047
B 0.042 0.042 0.042 0.042 0.043 0.044
C 0.045 0.044 0.046 0.044 0.044 0.045
D 0.045 0.045 0.045 0.045 0.043 0.048
E 0.045 0.046 0.047 0.045 0.046 0.047 0.046 0.048 0.047
F 0.904 0.758 0.839
G 0.812 0.726 0.729
H 0.773 0.753 0.792
 10 15 20 µg/ml

10 11 12
0.043 0.042 0.044 570

570

570

570

0.045 0.045 0.045 570

570

570

570

10 11 12
0.044 0.043 0.043 570

570

570

570

0.044 0.045 0.044 570

570

570

570

10 11 12
0.043 0.043 0.042 570

570

570

570

0.046 0.045 0.045 570

570

570

570

10 11 12
0.045 0.042 0.044 570
 570

570

570

0.044 0.045 0.046 570

570

570

570

10 11 12
0.043 0.044 0.042 570

0.54 0.052 0.054 570

0.428 0.053 0.053 570

0.136 0.054 0.053 570

0.044 0.045 0.044 570

0.81 0.711 0.914 570

0.727 0.738 0.761 570

0.819 0.757 0.818 570

10 11 12
0.043 0.042 0.044 570

0.254 0.053 0.054 570

0.225 0.054 0.051 570

0.163 0.059 0.055 570

0.044 0.044 0.046 570

0.672 0.686 0.828 570

0.674 0.664 0.807 570

0.665 0.75 0.846 570

10 11 12
0.043 0.044 0.042 570

570

570

570

0.045 0.045 0.045 570

0.662 0.605 0.615 570

0.701 0.687 0.606 570

0.663 0.619 0.623 570

10 11 12
0.045 0.046 0.043 570

570

570

570

0.053 0.047 0.045 570

0.88 0.824 0.89 570

0.801 0.796 0.881 570

0.791 0.777 0.863 570

24h(N1)
CBD 0 5 10 15 20 µg/ml DMSO 0
0.699 0.502 0.431 0.277 0.08 0.952

0.665 0.551 0.423 0.285 0.078 0.95

0.655 0.558 0.428 0.289 0.073 0.912

mean 0.938
0.673 0.537 0.427333 0.283667 0.077
% 100
100 79.79198 63.49678 42.14958 11.44131
เทียบกับ
DMSO 100 81.55998 65.66701 43.55818 12.07193

24h(N2)
CBD 0 5 10 15 20 µg/ml DMSO 0
0.888 0.812 0.579 0.427 0.081 0.897
0.846 0.801 0.602 0.285 0.076 0.883
0.84 0.828 0.545 0.387 0.08 0.904
mean 0.858 0.813667 0.575333 0.366333 0.079 0.894666667
% 100
100 94.83294 67.05517 42.69619 9.207459
เทียบกับ
DMSO 100 99.85548 77.20981 48.29186 10.53402

24h(N3)
CBD 0 5 10 15 20 µg/ml DMSO 0
1.237 1.072 0.872 0.614 0.094 0.899
1.144 1.205 0.772 0.58 0.092 0.889
1.172 1.024 0.751 0.616 0.091 0.851
mean 0.879666667
1.184333 1.100333 0.798333 0.603333 0.092333
% 100
100 92.9074 67.40782 50.94287 7.796229
เทียบกับ
DMSO 100 96.33895 78.29632 61.38732 9.686557

24h(N4)
CBD 0 5 10 15 20 µg/ml DMSO 0
1.407 1.352 0.953 0.557 0.073 0.898
1.414 1.027 0.847 0.657 0.069 0.82
1.237 1.27 0.718 0.492 0.08 0.853
mean 0.857
1.352667 1.216333 0.839333 0.568667 0.074
 % 100
100 89.92114 62.05027 42.04041 5.470675
เทียบกับ
DMSO 100 96.85264 69.75568 48.92979 6.448925
 5 10 15 20 µg/ml CBD
0.927 0.917 0.906 0.894 0 5
0.925 0.903 0.901 0.883 N1 100 81.55998
0.901 0.901 0.916 0.89 N2 100 99.85548
0.917666667 0.907 0.907667 0.889 N3 100 96.33895
97.83226724 96.6951 96.76617 94.77612 N4 100 96.85264
Mean 100 93.65176
Sd 0 7.109219
Cell death 0 6.348236

5 10 15 20 µg/ml
0.836 0.826 0.827 0.787
0.888 0.717 0.75 0.77
0.825 0.788 0.796 0.789
0.849666667 0.777 0.791 0.782
94.97019374 86.84799 88.41282 87.40686

5 10 15 20 µg/ml
0.781 0.763 0.728 0.716
0.876 0.739 0.733 0.679
0.888 0.77 0.729 0.729
0.848333333 0.757333 0.73 0.708

96.43804471 86.09322 82.98598 80.48503

5 10 15 20 µg/ml
0.789 0.749 0.802 0.798
0.765 0.783 0.729 0.701
0.833 0.755 0.678 0.682
0.795666667 0.762333 0.736333 0.727
92.84325165 88.95371 85.91988 84.83081
 10 15 20 µg/ml

65.66701 43.55818 12.07193

77.20981 48.29186 10.53402
78.29632 61.38732 9.686557
69.75568 48.92979 6.448925
72.73221 50.54179 9.685359
5.238917 6.596533 2.0549
27.26779 49.45821 90.31464
24h(N1)

CE1 0 1 5 10 25 50 µg/ml DMSO

0.905 0.799 0.647 0.182 0.088 0.055

0.892 0.816 0.637 0.163 0.056 0.053

0.882 0.848 0.786 0.164 0.076 0.055

mean mean
0.893 0.821 0.69 0.169667 0.073333 0.054333
% %
100 91.93729 77.26764 18.99963 8.212019 6.08436
เทียบกับ
DMSO 100 97.89586 92.1471 21.20942 8.976975 6.802889

24h(N2)
CE1 0 1 5 10 25 50 µg/ml DMSO
0.821 0.758 0.638 0.224 0.114 0.058

0.771 0.742 0.78 0.209 0.064 0.053

0.814 0.76 0.741 0.287 0.056 0.055

mean mean
0.802 0.753333 0.719667 0.24 0.078 0.055333
% %
100 93.93184 89.734 29.92519 9.725686 6.899418
เทียบกับ
DMSO 100 98.09337 93.28782 30.2526 10.27737 7.388807

24h(N3)
CE1 0 1 5 10 25 50 µg/ml DMSO
1.283 1.165 1.15 0.278 0.062 0.061
1.231 1.16 1.131 0.226 0.137 0.058
1.276 1.235 1.1 0.254 0.097 0.059
mean mean
1.263333 1.186667 1.127 0.252667 0.098667 0.059333
% %
100 93.9314 89.20844 20 7.810026 4.69657
เทียบกับ
DMSO 100 98.27852 91.60375 21.21856 8.357382 5.003479

24h(N4)
CE1 0 1 5 10 25 50 µg/ml DMSO
1.304 1.198 1.175 0.341 0.161 0.064
1.314 1.175 1.137 0.288 0.091 0.062
1.237 1.149 1.124 0.364 0.158 0.061
mean mean
1.285 1.174 1.145333 0.331 0.136667 0.062333
 % %
100 91.36187 89.131 25.75875 10.63554 4.850843
เทียบกับ
DMSO 100 99.65059 92.53539 27.24791 10.8762 5.176533
 0 1 5 10 20 50 µg/ml
0.974 0.857 0.755 0.834 0.869 0.861

0.902 0.837 0.78 0.804 0.83 0.8

0.917 0.929 0.807 0.864 0.856 0.837

0.931 0.874333333 0.780667 0.834 0.851667 0.832667

100 93.91335482 83.85249 89.5811 91.4787 89.43788

1
0 1 5 10 20 50 µg/ml
150
0.735 0.763 0.711 0.731 0.679 0.752
100

0.787 0.748 0.761 0.773 0.737 f(x) = − 1.75363211578626 x

50 0.702
+ 77.3225533414876
0
0.788 0.701 0.75 0.781 0.77 00.70310 20 30 40 50 60

0.77 0.737333333 0.740667 0.761667 0.728667 0.719

2
100 95.75757576 96.19048 98.91775 94.63203
150 93.37662
100

50 f(x) = − 1.8311501341843 x + 80.4242570403679

0
0 10 20 30 40 50 60

0 1 5 10 20 50 µg/ml
1.366 1.31 1.341 1.289 1.248 1.262 3
1.34 1.29 1.318 1.284 1.262
120 1.264
1.386 1.311 1.326 1.284 1.314
100 1.315
80
1.364 1.303666667 1.328333 1.285667 1.274667 1.280333
f(x) = − 1.79447352111738 x + 80.5194117714317
60
100 40
95.57673509 97.38514 94.25709 93.45064 93.86608
20
0
0 10 20 30 40 50 60

0 1 5 10 20 50 µg/ml
1.265 1.173 1.228 1.105 1.233 1.149
1.154 1.118 1.156 1.118 1.172 1.188
1.332 1.148 1.229 1.323 1.263 1.178
1.250333333 1.146333333 1.204333 1.182 1.222667 1.171667
100 91.68221808 96.32098 94.53479 97.78726 93.70834
CE1 0 1 5 10 25 50 µg/ml

N1 100 97.89586 92.1471 21.20942 8.976975 6.802889

N2 100 98.09337 93.28782 30.2526 10.27737 7.388807
N3 100 98.27852 91.60375 21.21856 8.357382 5.003479
N4 100 99.65059 92.53539 27.24791 10.8762 5.176533
Mean 100 98.47959 92.39352 24.98212 9.621982 6.092927
Sd 0 0.689485 0.613261 3.915015 1.002229 1.025917
Cell death 0 1.520415 7.606484 75.01788 90.37802 93.90707

N1 N2 N3

97.89586 99.65059 98.09337

1

5 92.1471 92.53539 93.28782

10 21.20942 27.24791 30.2526

25 8.976975 10.8762 10.27737

50 6.802889 5.176533 7.388807

Ic50 15.58109 16.61424 17.00697

mean 16.40077
sd 0.736518
24h(N1)

CE2 0 1 5 10 25 50 µg/ml DMSO

0.725 0.731 0.642 0.374 0.149 0.055

0.721 0.693 0.656 0.254 0.058 0.056

0.746 0.707 0.698 0.516 0.06 0.059

mean mean
0.730667 0.710333 0.665333 0.381333 0.089 0.056667
% %
100 97.21715 91.05839 52.18978 12.18066 7.755474
เทียบกับ
DMSO 100 98.43354 94.05344 54.19906 13.41083 8.84898

24h(N2)
CE2 0 1 5 10 25 50 µg/ml DMSO
0.836 0.823 0.697 0.474 0.054 0.057
0.912 0.768 0.738 0.578 0.056 0.056
0.859 0.777 0.752 0.299 0.059 0.057
mean mean
0.869 0.789333 0.729 0.450333 0.056333 0.056667
% %
100 90.83237 83.88953 51.82202 6.482547 6.520905
เทียบกับ
DMSO 100 96.74017 88.47502 52.1784 6.731356 6.161934

24h(N3)
CE2 0 1 5 10 25 50 µg/ml DMSO
0.674 0.668 0.523 0.415 0.057 0.058
0.719 0.677 0.533 0.403 0.06 0.062
0.711 0.663 0.547 0.421 0.062 0.06
mean mean
0.701333 0.669333 0.534333 0.413 0.059667 0.06
% %
100 95.43726 76.18821 58.88783 8.507605 8.555133
เทียบกับ
DMSO 100 99.61264 84.44755 61.46418 8.5977 9.774508

24h(N4)
CE2 0 1 5 10 25 50 µg/ml DMSO
0.745 0.788 0.594 0.402 0.06 0.062
0.814 0.719 0.662 0.406 0.08 0.06
0.784 0.717 0.639 0.403 0.063 0.06
mean mean
0.781 0.741333 0.631667 0.403667 0.067667 0.060667
 % %
100 94.92104 80.87921 51.68587 8.664106 7.767819
เทียบกับ
DMSO 100 99.08577 91.76757 53.48914 9.414281 8.783019
 0 1 5 10 20 50 µg/ml
0.722 0.724 0.684 0.662 0.605 0.615

0.678 0.645 0.647 0.701 0.687 0.606

0.704 0.709 0.706 0.663 0.619 0.623

0.701333333 0.692666667 0.679 0.675333 0.637 0.614667

100 98.76425856 96.81559 96.29278 90.827 87.64259

0 1 5 10 20 50 µg/ml
0.904 0.758 0.839 0.88 0.824 0.89
0.812 0.826 0.729 0.801 0.796 0.881
0.773 0.753 0.792 0.791 0.777 0.863
0.829666667 0.779 0.786667 0.824 0.799 0.878

100 93.89312977 94.8172 99.31699 96.30374 105.8256

0 1 5 10 20 50 µg/ml
0.685 0.615 0.657 0.653 0.662 0.582
0.677 0.639 0.636 0.677 0.749 0.607
0.642 0.666 0.515 0.59 0.572 0.565
0.668 0.64 0.602667 0.64 0.661 0.584667

100 95.80838323 90.21956 95.80838 98.9521 87.52495

0 1 5 10 20 50 µg/ml
0.754 0.741 0.661 0.722 0.707 0.638
0.788 0.745 0.7 0.745 0.702 0.675
0.742 0.702 0.652 0.74 0.693 0.707
0.761333333 0.729333333 0.671 0.735667 0.700667 0.673333
100 95.79684764 88.13485 96.62872 92.03152 88.44133
CE2 0 1 5 10 25 50 µg/ml

N1 100 98.43354 94.05344 54.19906 13.41083 8.84898

N2 100 96.74017 88.47502 52.1784 6.731356 6.161934
N3 100 99.61264 84.44755 61.46418 8.5977 9.774508
N4 100 99.08577 91.76757 53.48914 9.414281 8.783019
Mean 100 98.46803 89.6859 55.33269 9.538542 8.39211
Sd 0 1.081483 3.616466 3.613463 2.438009 1.345943
Cell deat 0 1.53197 10.3141 44.66731 90.46146 91.60789

1
120
100 3
80 f(x) = − 1.88512877 x 120
60 + 88.0985144
100
40
20 80
f(x) = − 1.89236349691853 x +
0 60
0 10 20 30 40 50 60
40

20
2
0
120 0 10 20 30
100
80
f(x) = − 1.88794896421 x
60 + 86.86862745013
40
20
0
0 10 20 30 40 50 60
 N1 N2 N3

98.43354 99.08577 96.74017

1

5 94.05344 91.76757 88.47502

10 54.19906 53.48914 52.1784

25 13.41083 9.414281 6.731356

50 8.84898 8.783019 6.161934

Ic50 20.2106 19.52911 18.22976

mean 19.32316
sd 1.006351

3
120

100

80
f(x) = − 1.89236349691853 x + 84.498390503447
60

40

20

0
0 10 20 30 40 50 60
24h(N1)

CE3 0 1 5 10 25 50 µg/ml DMSO

0.765 0.752 0.766 0.599 0.291 0.06

0.756 0.734 0.675 0.519 0.302 0.061

0.77 0.748 0.671 0.583 0.329 0.06

mean mean
0.763667 0.744667 0.704 0.567 0.307333 0.060333
% %
100 97.512 92.18682 74.24705 40.24443 7.90048
เทียบกับ
DMSO 100 98.73208 95.21898 77.10553 44.30889 9.014431

24h(N2) 120

CE3 0 1 5 10 25 50 µg/ml 100 DMSO

1.004 0.867 0.864 0.766 0.43 0.064 f(x) = − 1.90297046250708 x + 94.70
80
0.918 0.855 0.839 0.684 0.237 0.061
60
0.926 0.822 0.662 0.619 0.265 0.064
40
mean mean
0.949333 0.848 0.788333 0.689667 0.310667 0.063
20
% %
100 89.32584 83.04073 72.64747 32.72472 6.636236
0
เทียบกับ 0 10 20
DMSO 100 99.38848 87.57982 73.14707 33.98074 6.270915

24h(N3)
CE3 0 1 5 10 25 50 µg/ml DMSO
0.714 0.689 0.587 0.503 0.192 0.066
0.718 0.694 0.669 0.558 0.186 0.071
0.717 0.688 0.672 0.487 0.258 0.063
mean mean
0.716333 0.690333 0.642667 0.516 0.212 0.066667
% %
100 96.3704 89.71615 72.0335 29.59516 9.306654
เทียบกับ
DMSO 100 99.54963 99.44201 75.18497 29.90857 10.63314

24h(N4)
CE3 0 1 5 10 25 50 µg/ml DMSO
0.757 0.741 0.745 0.63 0.203 0.107
 0.766 0.731 0.708 0.602 0.285 0.147
0.759 0.711 0.74 0.643 0.168 0.061
mean mean
0.760667 0.727667 0.731 0.625 0.218667 0.105
% %
100 95.6617 96.09991 82.16477 28.74671 13.80368
เทียบกับ
DMSO 100 99.85892 109.0374 85.03141 31.23572 15.60773
 0 1 5 10 20 50 µg/ml
0.722 0.724 0.684 0.662 0.605 0.615

0.678 0.645 0.647 0.701 0.687 0.606

0.704 0.709 0.706 0.663 0.619 1

0.623
120
0.701333333 0.692666667 0.679 0.675333 0.637 0.614667
100
f(x) = − 1.8807300303546 x + 99.105268223858
100 98.76425856 96.81559 96.29278
80
90.827 87.64259

60

40
2
20

0
0 10 20 30 40 50 60
0 1 5 10 20 50 µg/ml
0.904 x + 94.7074664586593
f(x) = − 1.90297046250708 0.758 0.839 0.88 0.824 0.89

0.812 0.726 0.729 0.801 0.796 0.881

0.773 0.753 0.792 0.791 0.777 0.863

0.829666667 0.745666667 0.786667 0.824 0.799 0.878

100 89.87545199 94.8172 99.31699 96.30374 105.8256

10 20 30 40 50 60

3
120

0100 1 5 10 20 50 µg/ml
f(x) = − 1.92493319536566 x + 99.0456045696434
0.685 0.625 0.657 0.653 0.662 0.582
80
0.677 0.639 0.636 0.677 0.749 0.607
0.642 0.676 0.515 0.59 0.572 0.565
60
0.668 0.646666667 0.602667 0.64 0.661 0.584667
40
100 96.80638723 90.21956 95.80838 98.9521 87.52495
20

0
0 10 20 30 40 50 60

0 1 5 10 20 50 µg/ml
0.754 0.741 0.661 0.722 0.707 0.638
 0.788 0.745 0.7 0.745 0.702 0.675
0.742 0.702 0.652 0.74 0.693 0.707
0.761333333 0.729333333 0.671 0.735667 0.700667 0.673333

100 95.79684764 88.13485 96.62872 92.03152 88.44133

CE2 0 1 5 10 25 50 µg/ml

N1 100 98.73208 95.21898 77.10553 44.30889 9.014431

N2 100 99.38848 87.57982 73.14707 33.98074 6.270915
N3 100 99.54963 99.44201 75.18497 29.90857 10.63314
N4 100 99.85892 109.0374 85.03141 31.23572 15.60773
Mean 100 99.38228 97.81954 77.61725 34.85848 10.38155
Sd 0 0.411703 7.747364 4.503614 5.650368 3.396415
Cell deat 0 0.617722 2.180457 22.38275 65.14152 89.61845

N1 N2 N3

1 98.73208 99.38848 99.38228

5 95.21898 87.57982 97.81954

10 77.10553 73.14707 77.61725

25 44.30889 33.98074 34.85848

50 9.014431 6.270915 10.38155

Ic50 26.10996 23.49291 25.47977

mean 25.02754
sd 1.365877