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Training on Molecular Biology, DNA Fingerprinting and DNA Barcoding

First Batch 1st June. 2013 to 30th June. 2013

Second Batch 1st July 2013 to 30th July. 2013

Organized by
Escherichia Genomics
46 A second Floor IP Extention Hasanpur New Delhi 110092
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BACKGROUND
Realizing the importance of molecular biology techniques, DNA fingerprinting, DNA barcoding, and DNA diagnostics in human health care, molecular breeding, functional biomedical genetics, drug development and gene therapy with application of evolutionary studies and the genetic engineering. The Escherichia Genomics, New Delhi India was taken initiative at international level organizing Training on Molecular Biology, DNA Fingerprinting and DNA Barcoding (2013). The main aim of this training is to provide hands on experience to the various prospects of this new emerging field and share opportunities among scientists, engineers, doctors, research scholars and students in India and abroad. The training will provide an excellent platform for work and share them personnel with science, engineering, molecular biology, forensic, evolutionary biology, molecular breeding, genetically modified crop/organism/animal (GMOs, LMOs and transgenic) and medical background and help to come together, take the challenges in rapid development of this emerging field across the globe toward the evaluation and research development with biodiversity ,biotechnology, biosystematics, livestock genetic diversity, marine biodiversity, marine and fresh water fish biodiversity (fisheries biotechnology), ornithological studies, insects and pests diversity, agro entomology, integrated biological control , sericulture biotechnology, molecular medicine, intellectual property rights and patents, animal and veterinary products, animal nutrition and feed technology, conservation strategies and anthropological molecular survey studies. The Escherichia Genomics Training Program enhances opportunity for the development of the cellular, biochemical and molecular sciences with exclusive application biotechnology and molecular biology techniques towards the understanding the genomics and its wide phenomenon of development. Molecular Biology, DNA Fingerprinting And DNA Barcoding training is to provide trainees with a multidisciplinary conceptual and technical foundation for developing independent careers conducting transformative research in the biomedical sciences, life science and applied application based studies and commercially viable projects. The programspecific activity that is open to all interested research personnel. These yearly enrich students training by expose them to the full range of research being conducted by Escherichia Genomics professionals and their groups of
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Training Theme

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General Laboratory Experiment : (Hands on): DNA isolation from plant, bacteria, yeast, fungi , animal tissue (heart, kidney, lever, and blood etc), quantification and quality check of isolated DNA, DNA dilution, PCR amplification of DNA using multiplex primers, PCR amplification of DNA using random 10mer primers for RAPD, PCR amplification of DNA using SSR primers, micro satellite analysis and STR primer for DNA fingerprinting, separation following by electrophoresis, data capturing, data analysis and DNA fingerprint development. Human DNA Fingerprinting and Identification :Laboratory experiments (Hands on): DNA isolation from human blood, quantification and quality check of isolated DNA, DNA dilution, PCR amplification of DNA using multiplex primers, fragment (allele) separation following electrophoresis, data capturing, data analysis and DNA fingerprint development DNA fingerprinting of plants following Random Amplified Polymorphic DNA (RAPD) and microsatellite techniques: Laboratory experiments (Hands on): Isolation of DNA, quantification and quality check of isolated DNA, DNA dilution, PCR amplification of DNA using random 10 mer primers for RAPD, PCR amplification of DNA using SSR/ISSR/STR primers for microsatellite analysis, agarose gel electrophoresis of PCR products for RAPD, allele separation following automated capillary electrophoresis for microsatellite analysis, data collection and data analysis DNA barcoding of animals using Cytochrome C Oxidase subunit I (COI):Laboratory experiments (Hands on): DNA isolation, quantification and quality check of the isolated DNA, DNA dilution, PCR amplification of DNA using COI primers, agarose gel electrophoresis of PCR products, gel elution of PCR products, sequencing of PCR products and sequence data analysis. Forensic Molecular Biology: DNA fingerprinting method of plant, animals, microorganisms, human and diagnostics (disease diagnostics, heritable disease diagnostics, cancer diagnostics, pathogen diagnostics, parental diagnostic, and mass disaster of natural calamities and army causality. DNA Fingerprinting: Methods and protocols used in DNA fingerprinting of human, DNA fingerprinting of animals, DNA fingerprinting of fishes, DNA fingerprinting of plant, DNA fingerprinting of fungi, bacteria, and yeast etc. Implication and issues with techniques. DNA Bar-coding: Methods used in human DNA barcoding, animal DNA barcoding, insect DNA barcoding, fish DNA barcoding and microorganism DNA barcoding, plant DNA barcoding, implication and issues with techniques etc. Phylogenic Analysis: Molecular phylogenic analysis, genotyping of plant, animal, fish, insect and microbial population, individual and community, seed purity, genetic containment, germplasm characterization, allele and gene discovery, implication and issues with technique etc Genetic Engineering: Gene isolation, gene detection, GMOs, LMOs detection, GMO food detection, microbial contamination determination. Molecular Marker Technology: SSR, ISSR, RAPD, STR, molecular marker assisted selection, molecular breeding, cultivar development, molecular mapping, gene mapping, genetic structure analysis, molecular marker development. Biotechnology: Nucleic acid extraction, purification, dilution, spectrophotometer quantization, electrophoresis, DNA elution, PCR, PCR product purification, genetic (DNA) fingerprints, DNA molecular profile development. DNA Fingerprinting Software and Biostatistics Analysis: DNA fingerprinting profile regeneration, DNA barcoding, diversity matrix development, phylogenetic tree development, allele frequency, private allele, banding pattern, heterozygosis, primer discriminatory power test, and molecular biostatistics analysis(UPGMA,AMOVA,PCA, Njoin etc. Result Analysis and Interpretation: On forensic molecular biology, molecular genetics, evolutionary aspects and phylogenetic background.
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