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MICROBIAL FUEL CELLS (MFCs) Biofuels for energy production and Waste disposal Provosts Energy Retreat FEEI February 24 & 25, 2006 Ken Nealson Wrigley Professor of Geobiology USC
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PS Bacteria Cyanobacteria Algae & Plants
Geobiology
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Fermentation Metabolism
Geobiology
Biomass
CH3OH CH3CH2OH
3/1 2.5/1
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Geobiology
Dont always have to win breaking even might be enough!
Waste
Biofuel Cell Interruption of the process! (Imagine many other fuels being used by these Microbial Fuel Cells)
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Geobiology
What is a microbial fuel cell? Advantages of MFCs Disadvantages of MFCs State of the Art Challenges Prospects
Demonstrate these with examples of our work when appropriate.
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Geobiology
Oxidized Fuel
Oxidant
Oxidized Fuel
Oxidant
Cathode
Cathode
Anode
Anode
Microbe
Microbe Fuel
Fuel
Reduced Oxidant = Oxidized carrier molecule = Reduced carrier molecule = Ion Exchange Membrane = Outer membrane electron carriers (i.e. nanowires)
Almost any microbe can produce electricity with an electron shuttle (innefficient !) Mediators are mostly phenolic compounds, which are expensive & sometimes toxic A mediator-less microbial fuel cell is possible if the microbes can give electrons directly to the electrode
No additional electron shuttle is needed Few known microbes have this ability Shewanella & iron reducers do !
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Geobiology
Mediator-less fuel cells take advantage of special bacteria Isolated ~ 15 years ago -- Shewanella, Geobacter, others Iron/manganese reducing bacteria Famous for reducing solid substrates (Fe & Mn oxides) Subsequently found to have enzymes on outside of the cell Unusual for bacteria, but necessary for this reaction Example shown in next slide
Enrichment Culture
Solid Substrate
Pure Culture on MnO2 Breathing Mn oxide!
Biofilm
Anode
Injection port for fuel
Cathode
Scale in inches
Pt coated graphite Graphite felt felt electrode electrode Clamp holding ion exchange membrane
Used to evaluate strains in the laboratory Anode graphite with bacterial catalyst Glass autoclavable, re-usable Extra ports for electrochemical measurements Cathode platinized graphite (Surya Prakashs help!)
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Geobiology
Potential advantages of MFCs 1. Catalysts are inexpensive essentially free 2. Catalysts are diverse and robust extreme conditions of pH, Eh, T, salinity, etc. 3.Catalysts are versatile single type can use wide variety of substrates 4.Catalysts can self repair (proteins, DNA, membranes, etc.)
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More than 50 different Shewanella species known So far, all produce current From ~ 4 oC to 55 oC; wide salinity range 65 different carbon sources Very tough and robust organisms Just the tip of the iceberg of biological diversity (other Fe-reducers are known that grow to 110oC!)
MFC OD of 0.8
MR-1 can also use these products to maintain and produce current Can also switch from one to another with ease:
(1mM)
(1mM)
Form ate
(1mM)
Acetate
0.05 0.045 0.04 0.035 0.03 0.025 0.02 0.015 0.01 0.005 0 0
MFC OD of 0.3
Current (mA)
Lactate Lactate
Acetate
(1mM)
(1mM)
(1mM)
10 20 Tim e (hours) 30 40
0.05 0.045 0.04 0.035 0.03 0.025 0.02 0.015 0.01 0.005 0 0
Succinate
Tim e (Hours)
MFC OD of 0.3
Current (mA)
(1mM)
(1mM)
(1mM)
10 20 Tim e (hours) 30 40
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Geobiology
Almost certainly all these disadvantages are built on misconceptions These arise from use of mediated MFCs
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State of the art:
Geobiology
1. Many bacteria now known that produce current in mediator-less MFCs 2. Mechanism of current production not understood 3. Current densities are getting into the range of interest mA/cm2 (wide range of abilities) 4. Interesting development has to do with microbial consortia current density is always higher
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Geobiology
Chang et al., 2006, Electrochemically active bacteria (EAB) and mediator-less microbial fuel cells. J. Microbiol. Biotechnol. 16:163-177. Power densities range from: 16 to 4,300 mW/m2 I have a PDF of this I will send to anyone who wants reviews much of what I have said today.
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Geobiology
Challenges to be addressed: 1. How do they work? Mechanisms? genetic and genomic approaches 2. Physiology of the cells interface with FC biofilms, etc. 3. Microbial communities and consortia enrichment cultures
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Geobiology
9 mutants that knock out ability to produce current 4 are involved with iron reduction 5 are not 3 mutants that increase current production all of these are cytochromes leading to other termini Several regulatory mutants that increase or decrease the level of current production
luxSrif mtrA omcA hydB hydB and hydA hydA tatC mpw fur crp
Wild type
65.06.1 7.30.5 4.60.2 61.015.4 66.317.9 54.010.0 15.32.9 48.07.2 26.02.0 19.06.6 68.07.8 5.00.1
2.530.25 0.420.09 0.320.01 2.630.37 2.940.48 2.530.72 0.970.16 2.150.09 1.300.25 1.110.36 2.560.18 -
++ + +/+ + + ++ + + + + -
12
E.coli
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Geobiology
How do the catalysts work? Mutant screening genome of Shewanella has been sequenced use this information to make directed mutants mutant analysis identifies those genes coding for proteins involved with current prod. so far great success using this approach
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Geobiology
Understanding the catalyst: Role of attachment, biofilms, connections No doubt of catalytic ability Question of how to control and direct it This is issue of physiology of cells:
1.2
0.6
0.6
0.4
0.2
0.2
0 0 5 10 15 Time (hrs) 20 25 30
0 0 1 2 3 4 Time (Hours) 5 6 7 8
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Geobiology
Many questions to answer and things to optimize However, these approaches, coupled with modeling should lead to an optimum catalyst that can be combined with optimum design to yield high power To this end: we were just awarded a MURI From DOD for this work (5 from USC). (Prakash,Ronney,Wang,Mansfeld, Nealson)
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Prospects & Approach:
Geobiology
Understand the system Optimize to produce adequate current Scale up or down for specific applications: power waste disposal remote power supplies water treatment etc.
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Geobiology
Power Production
Scale up
Waste Disposal Water Treatment Biosensors
Research tool
Same scale
Teaching
MFC
Medical Devices
Scale down
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Waste Disposal:
Geobiology
7 billion tons of sewage sludge generated in the US We estimate that 90% of this could be metabolized by efficient MFC approach If properly designed, we could get paid for this process by current production THANK YOU !!
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Geobiology
MICROBIAL FUEL CELLS (MFCs) Biofuels for energy production and Waste disposal THANK YOU FOR YOUR ATTENTION !! Ken Nealson Wrigley Professor of Geobiology USC (knealson@usc.edu)
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Geobiology
Oxidant
Atmospheric oxygen
Catalyst
Microbe at anode Pt at cathode (soon to be microbe)
-e e
e-
eee- e e-
Cathode Catalyst
Fuel
Oxidation Reaction
e-
Oxidant
Reduction Reaction
Oxidized Fuel
H+ H+ + + H H+ H
H+ H+ + + H+H H+ H+ H
Reduced Oxidant
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Geobiology
e-
e-
e Fuel
Oxidation Reaction
eeAnode Catalyst
Cathode Catalyst
e-
Oxidized Fuel
H+ H+
+ H+ H+ H
H+ H+
+
Reduced Oxidant
H+H
H+ H+ H