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Figure 1: Genotype: Columbia 0M lateral root bud formation is not discernible because GUS was not present

Figure 1a 100 nM lateral root bud formation is not discernible because GUS was not present

Figure 1b 5 microM lateral root bud formation is not discernible because GUS was not present

Figure 1c 10 microM lateral root bud formation is not discernible because GUS was not present

Figure 1d 100 microM lateral root bud formation is not discernible because GUS was not present

Figure 1e

Figure 2: Genotype: CyclinB1::GUS 0M blue precipitate in leaves and at 2-3 points per plant on roots, marking lateral root bud initiation

Figure 2a 100 nM more blue precipitate along roots

Figure 2b 5 microM blue precipitate throughout the entire plant (uniformly blue)

Figure 2c 10 microM greater amount of blue precipitate throughout the plant (dark blue)

Figure 2d 100 microM greatest amount of precipitate throughout the plant (darkest color blue)

Figure 2e

Figure 3: Genotype: LBD-16::GUS 0M evidence of blue precipitate throughout the plant (GUS is expressed) lateral roots are visible at one location per plant Figure 3a 100 nM evidence of blue precipitate marking GUS expression throughout the plant more lateral roots are visible, some longer than 0 M auxin Figure 3b 5 microM evidence of blue precipitate marking GUS expression throughout the plant increase amount of lateral root formation at half-length of the main root Figure 3c 10 microM evidence of blue precipitate marking GUS expression throughout the plant GUS expression throughout the plant is very strong (dark blue), especially in the roots Figure 3d 100 microM evidence of blue precipitate marking GUS expression throughout the plant GUS expression throughout the plant is vary strong, both in roots and leaves Figure 3e

Figure 4: Genotype: LBD-33::GUS 0M evidence of blue precipitate marking GUS expression 2-3 points of localized blue precipitate in roots (per plant) marking lateral root initiation Figure 4a 100 nM evidence of blue precipitate marking GUS expression long tracks of blue along the roots of each plant, marking more extensive lateral root initiation Figure 4b 5 microM evidence of blue precipitate marking GUS expression large swaths of root which exhibit strong expression of GUS, marking more extensive lateral root formation 10 microM evidence of blue precipitate marking GUS expression entire root length exhibits GUS expression, with several areas showing stronger lateral root formation than others Figure 4d 100 microM evidence of blue precipitate marking GUS expression entire roots and shoots exhibit a deep blue coloring, marking lateral root formation along their length Figure 4e

Figure 4c

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