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Supercoiling: coiling of the ds DNA helix upon itself (higher order coiling of the DNA helix). 3.1 3.2 3.3 3.4 Polyoma virus experiments Circular DNA; supercoiled DNA Biological consequences The concept of supercoiling: linking number and linking number difference Twist and writhe: Whites theorem
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Vinograd et al. (1965) PNAS USA 53: 1104-1111 March 16, 2010 Math 414-1 / 714-1 Lecture 13 1
3.2 DNA in nature is found in circular form (plasmids) or as structures topologically equivalent to circles
3.3. Supercoiling naturally appears in many biological processes such as DNA replication
Bates and Maxwel, DNA topology, 2005 Oxford University Press March 16, 2010 Math 414-1 / 714-1 Lecture 13 3
Prereplicated domain
Who takes care of Supercoiling in the cell? Enzymes; type I and type II topoisomerases
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Intuitively: Supercoiling happens due to an excess of the number of times that the two DNA backbones coil around each other. Note to the students: the following slides are vague and should be complemented with your lecture notes
March 16, 2010 Math 414-1 / 714-1 Lecture 13 11
Consider a linear fragment of DNA: N=Number of base-pairs of the fragment h=Number of base pairs/helical turn Lk0=N/h~ #helical turn Consider a planar circular molecule : Lkm= Closest integer to N/h
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+1
+1
-1 +1
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+1
+1 c1
-1 +1 c2
Lk=1/2( sign(ci))
March 16, 2010 Math 414-1 / 714-1 Lecture 13 16
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Supercoiling
Linking number difference: Lk=Lk- Lk0 Why Lk0 instead of Lkm?
Geometrical parameters
Twist: Measures the coiling of the two individual strands. Tw0=N/h for open circular not necessarily planar due to intrinsic curvature. Writhe: Describes how the helix axis coils in space. Wr0=due to intrinsic curvature
Math 414-1 / 714-1 Lecture 13
Calugareanu-White-Fuller Theorem
Lk=Tw+Wr
March 16, 2010 Math 414-1 / 714-1 Lecture 13 21 J. Arsuaga, math 195-3
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