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Cre PCR Template

encoding Cre.

in vitro transcription template for large-scale synthesis of mRNA

he Stealth Express Cre PCR template for In vitro transcription allows for the large-scale synthesis of mRNA encoding Cre recombinase. With the mRNA product, exogenous Cre expression can be achieved when transfected into target cells. This new gene delivery technology is faster and more efficient than conventional virus-based approaches. 10 g of template is provide, enough to make hundreds of micrograms of mWasabi encoding mRNA. Within each template, the coding sequence for a protein is flanked by 5 and 3 untranslated regions designed to maximize the translational efficiency and cytoplasmic stability of the mRNA. The template drives incorporation of 120-nt polyA tails into RNA transcripts, eliminating the need to enzymatically polyadenylate IVT products before use. he bacterial phage P1 Cre/lox recombination system mediates DNA excision, integration, inversion and translocation in a sequence specific manner involving only two genetic components: the 38-kDa Cre (cyclization recombination) recombinase and two 34 bp loxP (locus of X-over of P1) sites. The most efficient Cre/lox reaction is DNA excision from a floxed locus (flanked by loxP) and this reaction has been frequently applied for mouse genome modification.

Box 1 | Product Info


Content Qty

Cat#: ABP-RP-SECRE10 Cre linear DNA template ready to use directly in IVT reactions. 10 g

Storage -20C

Features
Template contains the T7 phage polymerase promoter allowing the widely available T7 polymerase to be used. Inherently non-mutagenic PCR template for footprint-free gene expression Reliable expression levels Eliminates safety concerns and bio-containment issues associated with virus

Box 2 | Components Needed for IVT


Component
Modified UTP

Cat. No.
ABP-PP-NTPMODU

Description
rNTPs for In vitro transcription. Modified Uracil is required to ameliorate immunological responses towards the mRNA product. rNTPs for In vitro transcription. Modified Cytosine is required to ameliorate immunological responses towards the mRNA product. rNTPs for in vitro transcription. rNTPs for in vitro transcription. RNA polymerase for In vitro Transcription Anti-Reverse Cap Analog; transcripts produced with ARCA have increased translation in comparison with conventional cap analogs.

Modified CTP

ABP-PP-NTPMODC

Standard ATP Standard GTP T7 polymerase ARCA

ABP-PP-NTPSTDA ABP-PP-NTPSTDG ABP-PP-T7POL ABP-PP-NTPARCA

or Research Use Only. Not for Diagnostic or Therapeutic Use. Purchase does not include or carry any right to resell or transfer this product either as a standalone product or as a component of another product. Any use of this product other than the permitted use without the express written authorization of Allele Biotech is strictly prohibited

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