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Cre PCR Template in vitro transcription template for large-scale synthesis of mRNA encoding Cre. T

Cre PCR Template

in vitro transcription template for large-scale synthesis of mRNA encoding Cre.

T he Stealth Express Cre PCR template for In vitro tran- scription allows for the large-scale synthesis of mRNA

encoding Cre recombinase. With the mRNA product, exog- enous Cre expression can be achieved when transfected into target cells. This new gene delivery technology is faster and more efficient than conventional virus-based approach- es. 10 μg of template is provide, enough to make hundreds of micrograms of mWasabi encoding mRNA. Within each template, the coding sequence for a protein is flanked by 5’ and 3’ untranslated regions designed to maximize the trans- lational efficiency and cytoplasmic stability of the mRNA. The template drives incorporation of 120-nt polyA tails into RNA transcripts, eliminating the need to enzymatically poly- adenylate IVT products before use.

T he bacterial phage P1 Cre/lox recombination system mediates DNA excision, integration, inversion and trans-

location in a sequence specific manner involving only two genetic components: the 38-kDa Cre (cyclization recombi- nation) recombinase and two 34 bp loxP (locus of X-over of P1) sites. The most efficient Cre/lox reaction is DNA excision from a “floxed” locus (flanked by loxP) and this reaction has been frequently applied for mouse genome modification.

Box 1 | Product Info Content Qty Cat#: ABP-RP-SECRE10 Cre linear DNA template ready to
Box 1 | Product Info
Content
Qty
Cat#: ABP-RP-SECRE10
Cre linear DNA template ready to
use directly in IVT reactions.
10 μg
Storage
-20 ° C
Features

Template contains the T7 phage polymerase promoter

allowing the widely available T7 polymerase to be used.

Inherently non-mutagenic PCR template for footprint-free gene expression

Reliable expression levels

♦ Eliminates safety concerns and bio-containment issues associated with virus Box 2 | Components Needed
♦ Eliminates safety concerns and bio-containment issues
associated with virus
Box 2 | Components Needed for IVT
Component
Cat. No.
Description
Modified UTP
ABP-PP-NTPMODU
rNTPs for In vitro transcription. Modified
Uracil is required to ameliorate immunological
responses towards the mRNA product.
Modified CTP
ABP-PP-NTPMODC
rNTPs for In vitro transcription. Modified Cy-
tosine is required to ameliorate immunological
responses towards the mRNA product.
Standard ATP
ABP-PP-NTPSTDA
rNTPs for in vitro transcription.
Standard GTP
ABP-PP-NTPSTDG
rNTPs for in vitro transcription.
T7 polymerase
ABP-PP-T7POL
RNA polymerase for In vitro Transcription
ARCA
ABP-PP-NTPARCA
Anti-Reverse Cap Analog; transcripts produced
with ARCA have increased translation in com-
parison with conventional cap analogs.

F or Research Use Only. Not for Diagnostic or Therapeutic Use.

Purchase does not include or carry any right to resell or transfer this product either as a stand-

alone product or as a component of another product. Any use of this product other than the permit- ted use without the express written authorization of Allele Biotech is strictly prohibited