Names: Shannen Christelle A. Andrade, Bea Luzia G. Ebreo, Katrina SJ.

Santos Section: III Calvin Date: February 1, 2010 Advanced Biology Worksheet #1

1. Microbial Diseases of the Integumentary System Causative Microbe Bacterial Known Cellulitis Diseases - caused by a type of bacteria entering the skin, usually by way of a cut, abrasion, or break in the skin. This break does not need to be visible. Group A Streptococcus and Staphylococcus are the most common of these bacteria Acne Propionibacteriu m acnes (P. acnes) is the anaerobic bacterium that causes acne. Boil - Boils are red, pus-filled lumps that are tender, warm, and extremely painful. The cause is bacteria such as staphylococci.

Fungal Athletes Foot/ Tinea Pedis - skin between the toes becomes itchy and sore, cracking and peeling away Ringworm/ Tinea -a contagious fungus infection that can affect the scalp and other body parts. Tinea versicolor - causes discolored patches of skin.

Viral Warts - are small growths. The virus infects the surface layer. Warts are contagious. Chickenpox - caused by primary infection with varicella zoster virus (VZV). It is spread easily through coughs or sneezes of ill individuals, or through direct contact with secretions from the rash. Herpes Zoster/Shingles - characterized by a painful skin rash with blisters in a limited area on one side of the body, often in a stripe. It is also caused by VZV

Protozoan Toxoplasmosis Leishmaniasis - caused by the protozoan Toxoplasma gondii. It is an infection that passes from animals to humans, sometimes without causing any symptoms. Dracunculiasis/ Guinea Worm Disease - caused by the nematode Dracunculus medinensis. The name dracunculiasis is derived from the Latin "affliction with little dragons"

Treatment / Prevention

A. Prevention - Don't overwash. Skip harsh scrubs. Say no to alcohol. Don't squeeze or pick. Wash face. Cellulitis B. Treatment - resting the affected limb or area, cleaning the wound site if present (with debridement of dead tissue if necessary) and treatment with oral antibiotics, except in severe cases, which may require admission and intravenous (IV) therapy. Acne B. Treatment - Gently washing the affected area(s) with warm water and a mild soap twice a day to remove dead skin cells and excess oil -Using a topical (applied to the skin) overthe-counter acne treatment containing benzoyl peroxide or salicylic acid Boil

A. Prevention - Avoid sharing things. Take a bath daily. Keep the area dry. Athletes Foot/ Tinea Pedis B. Treatment -keep the infected area clean and dry. Buy shoes that are leather or other breathable material. Use powder. use antifungal creams and washes. Ringworm/ Tinea B. Treatment -Topical treatment: use antifungal creams -Systemic treatment: use of oral medication. Use antifungal tablets like griseofulvin Tinea versicolor B. Treatment -use over-thecounter drugs like clotrimazole and miconazole

A. Prevention - Practice good personal hygiene. Vaccination. Warts B. Treatment -use topical treatments containing salicylic acid. Others are podophyllum resin paint, Imiquimod, Dinitrochlorobenz ene, and others. Chickenpox B. Treatment -healthy individuals may only need home treatment. They should rest and take acetaminophen (such as Tylenol) or ibuprofen (such as Advil) to reduce fever and discomfort. They should also see a doctor or health professional. Herpes Zoster/Shingles B. Treatment - The affected area should be kept clean. Cool compresses and anti-itching lotions, such as calamine lotion, may also provide relief. An

A. Prevention -Avoid contact with unsanitary objects like feces. Cook meat thoroughly. Wash food before eating. Always filter drinking water Toxoplasmosis Leishmaniasis B. Treatment -for healthy people it will not need treatment. For others, medicines such as Pyrimethamine (Daraprim) and sulfudiazine is prescribed. Dracunculiasis/ Guinea Worm Disease B. Treatment - There is no vaccine or medicine to treat or prevent Guinea worm disease. Once a Guinea worm emerges a person must wrap the live worm around a piece of gauze or a stick to extract it from the body.

B. Treatment - Applying warm compresses to a boil can help it to drain. Large boils should be incised and drained. Antibiotic therapy is advisable for large or recurrent boils or those that occur in sensitive areas (such as around or in the nostrils or in the ear). Drawing/ Illustratio n Cellulitis Athletes Foot

aluminum acetate solution can be used to help dry up the blisters and oozing. Drugs like acyclovir (Zovirax) reduce the severity and duration of the rash if started early

Warts

Toxoplasmosis Leishmaniasis

Acne

Ringworm

Chickenpox

Dracunculiasis/ Guinea Worm Disease

Boil

Tinea Versicolor

Herpes Zoster

Advanced Biology Worksheet #2 2. Microbial Diseases of the Nervous System

Causative Microbe Bacterial Known Bacterial Diseases Meningitis -an inflammation of the meninges, the membranes that surround the brain. Caused by Neisseria meningitidis

Fungal Mucormycosis -caused by fungi in the order Mucorales, and Mucor, Rhizopus, Absidia, and Cunninghamella species

Fungal Meningitis Lyme Disease -infection that - caused by a causes swelling bacterium called a and irritation of "spirochete," the tissue around which may be the brain and transmitted to spinal cord. humans by the bite of infected ticks (Ixodes scapularis and Ixodes pacificus).

Viral Poliomyelitis -caused by a picornavirus and is found only in humans. It is transmitted by ingestion of fecally contaminated water. Rabies -Caused by a rhabdovirus. It is contracted through the bite of rabid animal, by inhalation of aerosols, or invasion through minute skin abrasions. Viral Meningitis -most common form and is transmitted via oral-fecal route and is usually self- limiting.

Protozoan African Trypanosomiasis -caused by Trypanosoma brucei gambiense and Trypanosoma brucei rhodesiense and is transmitted by the bite of the tsetse fly (Glossina).The disease affects the nervous system causing lethargy and eventual coma. It is commonly called sleeping sickness. Naegleria Meningoencepha litis / Encephalitis -caused by the protozoan Naegleria fowleri is almost always fatal. It is contracted from stream or pond water and children are the most common victims. Primary amoebic meningoencepha litis

Tetanus - Clostridium tetani produces the tetanus toxin which results in continuous muscle contraction, can lead to systemic organ failure and death

Treatment / Prevention

A. Prevention - Prevention through vaccination. Avoid close contact. Spray insect (tick) repellants. Bacterial Meningitis B. Treatment - Early diagnosis and treatment are very important. If symptoms occur, the patient should see a doctor right away. Bacterial meningitis can be treated with a number of effective antibiotics. It is important, however, that treatment be started early. Lyme Disease B. Treatment - require intravenous drugs; examples are ceftriaxone (Rocephin) and penicillin G. For pain-relieving, the doctor will remove fluid in

A. Prevention - Vaccination. Have a good hygiene. Avoid sharing drinking glasses, water bottles, eating utensils, and others. Wash hands often with soap. Eat meat that is thoroughly cooked. Mucormycosis B. Treatment Complete treatment of underlying medical disease. Correct hypoxia, acidosis, hyperglycemia, and electrolyte abnormalities. Fungal Meningitis B. Treatment - Intravenous therapy with amphotericin B is the most common treatment. Use antifungal medicine.

- disease of the central nervous system caused by infection from Naegleria fowleri. A. Prevention A. Prevention - Vaccination. - Sanitation of Avoid direct surroundings. contact to unfamiliar African things. Trypanosomiasis B. Treatment Poliomyelitis - intravenous B. Treatment (IV) therapy. Use -use the drug Eflornithine. antibiotics. See the doctor to check your health. Use pain Naegleria Meningoencepha killers. Physical litis / therapy, braces Encephalitis or corrective B. Treatment shoes, or -you will be orthopedic treated in a surgery to help hospitals recover muscle intensive care strength and unit. Your vital function. signs will be closely Rabies monitored. B. Treatment -Clean the wound bitten by Primary amoebic the rabid animal. meningoencepha litis You will be given a series of B. Treatment - prompt shots called intravenous postexposure administration of prophylaxis heroic doses of (PEP) Amphotericin B Viral Meningitis B. Treatment Dexamenthason

the joints through arthrocentesis. use oral medications such as ibuprofen to reduce inflammation and improve function. Tetanus B. Treatment Tetanus part of DPT vaccine, given pre-made antitoxin antibodies which will bind and neutralize toxin (passive immunization) plus a booster shot of the DT vaccine Bacterial Meningitis

(Decadron) is given via IV. Management is supportive since it is selflimiting.

Drawing/ Illustratio n

Mucormycosis

Poliomyelitis

African Trypanosomiasis Blood smear

Lyme Disease

Fungal Meningitis Rabies Dog with rabies

Naegleria Meningoencephalitis

Tetanus

Primary amoebic meningoencephalitis

Viral Meningitis

Advanced Biology Worksheet #3

3. Microbial Diseases of the Cardiovascular System

Causative Microbe Known Diseases

Bacterial Sepsis and Septic Shock Streptococcus pyogenes is the most frequent cause. It is the growth of microorganisms in blood. Subacute bacterial endocarditis - caused by alpha-hemolytic streptococci, but may also be caused by staphylococci or enterococci. Acute bacterial endocarditis - caused by Staphylococcus aureus (or Streptococcus pyogenes).The bacteria cause rapid destruction of heart valves and is frequently fatal within days or weeks.

Fungal Fungemia/ Candidemia - presence of fungi or yeasts in the blood

Viral Myocarditis -caused by coxsackievirus. The virus infects the respiratory or gastrointestinal tract and spreads to the heart through blood.

Yellow Fever -caused by a virus (yellow fever virus). The American vector is the mosquito Aedes Trypanosomiasis aegypti. (Chagas Disease) Hantavirus - caused by pulmonary infection with the syndrome protozoan -caused by hantavirus. The parasite virus is Trypanosoma contracted by cruzi. The inhalation of organism T cruzi dried rodent and infection in urine. humans were first described in 1909 by the Brazilian physician Carlos R. J. Chagas. A. Prevention A. Prevention

Protozoan Malaria - an infectious disease caused by a parasite (plasmodium) which is transmitted from human to human by the bite of infected female Anopheles mosquitoes.

Treatment/

A. Prevention

Prevention

-Antibiotics help prevent bacteremia and infections from developing. Sepsis and Septic Shock B. Treatment -must be treated immediately with antibiotics even before test results confirm the diagnosis; surgery. Subacute bacterial endocarditis B. Treatment -requires eradication of all microorganisms from the vegetation(s), usually on the heart valve; requires hospitalization and antibiotic therapy.

-Vaccination; -eradication of mosquito breeding sites for control insects Myocarditis B. Treatment - Patients with mild viral myocarditis can rest at home. A patient with severe myocarditis and produces heart failure or cardiac arrhythmias needs to be treated and monitored in a hospital. Yellow Fever B. Treatment -no actual treatment. The person must only take sufficient rest and supplement the lost of bodily fluids Hantavirus pulmonary syndrome B. Treatment - No actual treatment. Although there has been some experimental use of the antivirus drug, ribavirin, mechanical Malaria B. Treatment - Drugs include chloroquine, mefloquine, primaquine, quinine, pyrimethaminesulfadoxine (Fansidar), and doxycycline. -Seek medical help American Trypanosomiasis (Chagas Disease) B. Treatment - may include medications, a pacemaker or other devices to regulate your heart rhythm, surgery, or even heart transplant.

Acute bacterial endocarditis B. Treatment - Combining surgery and medical treatment

Drawing/ Illustration

Sepsis and Septic Shock

ventilation (use of a respirator) is the main treatment. Most patients need to be hospitalized in intensive care Myocarditis

Malaria RBC when there is infection of malaria

Yellow Fever American Trypanosomiasis (Chagas Disease) Subacute bacterial endocarditis Hantavirus pulmonary syndrome

Acute bacterial endocarditis

Advanced Biology Worksheet #5 1. Microbial Diseases of the Digestive System Causative Microbe Known Diseases

Bacterial
1.Staphylococcal Food poisoning- a leading cause of gastroenteritis. It is an intoxication caused by ingesting an enterotoxin produced by S. aureus. Staphylococci are comparatively resistant to environmental stresses. 2.Shigellosis (Bacillary Dysentery)- is a severe form of diarrhea caused by a group of facultatively anaerobic gramneagtive rods of the genus Shigella. 3.Salmonellosis (Salmonela Gastroenteritis)the Salmonella bacteria are gramnegative, facultatively anaerobic, nonendospore-forming rods. Their normal habitat is the intestinal tracts of humans and many animals. All salmonellae are considered

Fungal
1.Ergot Poisoningsome mycotoxins are produced by Claviceps purpurea, a fungus causing smut infections on grain crops. The mycotoxins produced by C. purpurea cause ergot poisoning, or ergotism, which results from the ingestion of rye or other cereal grains contaminated with the fungus.

Viral
1.Mumps- typically begins with painful swelling of one or both parotid glands 16-18 days after exposure to the virus. The virus is transmitted in saliva and respiratory secretions, and its portal of entry is the respiratory tract.

Protozoan
1.GiardiasisGiardia lamblia is a flagellated protozoan that is able to attach firmly to a humans intestinal wall. Giardiasis is a prolonged diarrheal disease caused by Giardia lamblia.sometimes persisting for weeks, giardiasis is characterized by malaise, nausea, flatulence, weakness weight loss, and abdominal cramps. 2.Cryptosporidio sis- is caused by the protozoan Cryptosporidium parvum, which was not recognized as a pathogen of humans until 1976. infection occurs when humans ingest the cryptosporidian oocysts. The oocysts eventually release sporozoites in the small intestine. 3.Amoebic Dysentery- or amoebiasis, is

2.Hepatitis- is an inflammation of the liver. Viral hepatitis is now the second most frequently reported infectious 2.Aflatoxin disease in the United PoisoningStates. At least five Aflatoxin is a mycotoxin produced different viruses cause hepatitis, and by the fungus Aspergillus flavus, a probably more remain to be common mold. discovered or Aflatoxin has been found in many foods become better known. Hepatitis is but particularly likely to be found on also an occasional result of infections peanuts. Aflatoxin poisoning can cause by other viruses such as Epstein-Barr virus serious damage to livestock when their or cytomegalovirus. feed is contaminated 3.Viral Gastroenteritis- is with A. flavus. one of the most common diseases of humans, and about 90% of cases of viral gastroenteritis are caused by either the rotavirus or the human calciviruses,

pathogenic to some degree, causing salmonellosis.

better known as the Norwalk family of viruses; collectively, the noroviruses.

Treatment / Preventio n

1. For most patients, staphylococcal food poisoning causes a brief illness. The best treatments for these patients are rest, plenty of fluids, and medicines to calm their stomachs. Highly susceptible people, such as the young and the elderly, are more likely to have severe illness requiring intravenous therapy and care in a hospital. Antibiotics are not useful in treating this illness. The toxin is not affected by antibiotics. 2.In severe cases of shigellosis, antibiotic theraphy and oral rehydration are indicated. At present, fluoroquinolones are the antibiotics of choice. 3. Salmonella infections usually resolve in 5-7 days and often do not

1.Avoid eating grain products contaminated with the fungus. 2.Decontamination, symptomatic and supportive measures

1. There is no specific treatment for mumps. Symptoms may be relieved by the application of intermittent ice or heat to the affected neck area and by acetaminophen/parac etamol (Tylenol) for pain relief. Aspirin is not used due to a hypothetical link with Reye's syndrome. Warm salt water gargles, soft foods, and extra fluids may also help relieve symptoms. Patients are advised to avoid fruit juice or any acidic foods, since these stimulate the salivary glands, which can be painful. 2. Doctors often combine different antiviral drugs to better combat the virus. For example, interferon treatment may often be combined with other antiviral drugs like Lamivudine in the case of hepatitis B infection or Ribavirin for people with hepatitis C infection. The drug combinations have a

spreadmostly by food or water contaminated by cysts of the protozoan amoeba Entamoeba histolytica. 1.Treatment with metronidazole or quinacrine hydrochloride is usually effective within a week. 2.Microscopic examination can identify the oocysts, which are about twice the diameter of those of Cryptosporidium. There is really no satisfactory test for contamination in foods. The antibiotic combination of trimethoprim and sulfamethoxazole is used for treatment. 3. Amoebic dysentery can be treated with metronidazole but must be followed up with a second lumenal drug to eliminate amoebae from the intestine.

require treatment other than oral fluids. Persons with severe diarrhea may require rehydration with intravenous fluids. Antibiotics, such as ampicillin, trimethoprimsulfamethoxazole, or ciprofloxacin, are not usually necessary unless the infection spreads from the intestines. Some Salmonella bacteria have become resistant to antibiotics, largely as a result of the use of antibiotics to promote the growth of food animals.

stronger therapeutic effect than a singular drug on its own. Similar to interferon, the side effects can be severe. However, the costs of this combination therapy are relatively reasonable. 3.The only treatment for viral gastroenteritis is oral rehydration or, in exceptional cases, intravenous rehydration.

Drawing/ Illustratio n

Shigella- causes Shigellosis (Bacillary Dysentery).

Aspergillus flavuscauses Aflatoxin Poisoning.

Rotavirus- causes Viral Gastroenteritis.

Cryptosporidium parvum- causes Cryptosporidiosis.

Advanced Biology Worksheet #7 2. Microbial Diseases of the Reproductive System Causative Microbe Known Diseases

Bacterial
1.Gonorrhea-an STD caused by the gram-negative diplococcus Neisseria gonorrhoeae. -Males become aware of a gonorrheal infection by painful urination and a discharge of pus-containing material from the urethra. -In females, the disease is more insidious. Only the cervix, which contains columnar epithelial cells, is infected. -Complications of gonorrhea can involve the joints, heart, meninges, eyes, pharynx, or other parts of the body. 2.Pelvic Inflammatory Disease(PID)- is a collective term for any extensive bacterial infection of the female pelvic organs, particularly the uterus, cervix, uterine tubes, or ovaries.

Fungal
1.Candidiasisvaginal infections by yeastlike fungi of the genus Candida are responsible for millions of physician office visits every year. By the time they reach the age of 25, an estimated half of college will have at least one physiciandiagnosed episode.

Viral
1.Genital Herpesa much publicized STD, usually caused by herpes simplex virus type 2. Herpes simplex virus type 1 is primarily responsible for cold sores or fever blisters, but it can also cause genital herpes. 2.Genital Wartswarts are an infectious disease; since 1907 it has been known that they are caused by viruses known as papillomaviruses. It is probably less well known that warts can be transmitted sexually, and that is an increasing problem.

Protozoan
1.Trichomoniasisthe anaerobic protozoan Trichomonas vaginalis is frequently a normal inhabitant of the vagina in females and of the urethra in many males. If the normal acidity of the vagina is disturbed, the protozoan may overgrow the normal microbial population of the genital mucosa and cause trichomoniasis.

3. AIDS- Acquired virally induced immunodeficiency, caused by HIV retrovirus.

Treatment/ Prevention

3.Bacterial VaginosisInflammation of the vagina due to infection, or vaginitis, is most commonly caused by one of three organisms: the fungus Candida albicans, the protozoan Trichomonas vaginalis, or the bacterium Gardnerella vaginalis, a small, pleomorphic gramvariable rod. Most of these cases are attributed to the presence of G. vaginalis and are termed bacterial vaginosis. 1.Antibiotic resistance to the gonococcus has recently accelerated at an alarming rate. Although penicillin has been an effective treatment of gonorrhea for many years, the dosages have had to be increased substantially because of penicillin resistant bacteria. The current CDC recommendation for treatment of gonorrhea, which is periodically subject to change, is to use fluorquinolone antibiotics such as

1.Treatment usually consists of topical application of nonprescription antifungal drugs such as clotrimazole and miconazole. An alternative treatment is a single dose of oral fuconazole or other azole-type antifungal.

1.There is no cure for genital herpes, although research on its prevention and treatment is insensitive. Discussions of chemotheraphy use terms such as suppression or management rather than cure.currently, the antiviral drugs acylclovir, famciclovir, and valacyclovir are recommended for treatment. 2.Two patientapplied gels, podofilox and imiquimod, are often useful treatments.

1.Treatment is by oral metronidazole, administered to both sex partners, which readily clears the infection.

ciprofloxacin; thare inexpensive and require only a single dose. 2.The recommended treatment for PID is the simultaneous administration of doxycycline and cefoxitin. This combination is active against both the gonococcus and Chlamydia. 3.Treatment is primarily by metronidazole, a drug that eradicates the anaerobes essential to continuation of the disease but allows the normal lactobacilli to repopulate the vagina.

Imiquimod stimulates the body to produce interferon, which appear to account for its antiviral activity.

3. No treatment is effective, it is usually fatal due to opportunistic infection.

Drawing/ Illustration

Neisseria gonorrhoeae causes Gonorrhea.

Candida albicans - causes Candidiasis.

Papillomavirus causes Genital Warts.

Trichomonas vaginalis- causes Trichomoniasis.

Advanced Biology Worksheet #9 3. Microbial Diseases of the Sensory organs

Causative Microbe Bacterial Known Diseases 1.Neonatal

gonorrheal ophthalmia- is a serious form of conjunctivitis caused by Neisseria gonorrhoeae. Large amounts of pus are formed; if treatment is delayed, ulceration of the cornea will usually result. 2.Inclusion Conjunctivitis- is quite common today. It is caused by Chlamydia trachomatis, a bacterium that grows only as an obligate intracellular parasite. 3.Trachoma- a serious infection caused by C. trachomatis. the greatest single cause of blindness in the world today.

Fungal
1. Fungal keratitis- is a disease that results from the fungus Fusarium, found in plants and soil. The infection is highly dangerous and can lead to permanent blindness. 2. Fungal RhinosinusitisFungal sinusitis is rare, although it is possible that its presence is often not recognised by clinicians as the organisms responsible are not easy to culture. The incidence of fungal rhinosinusitis varies from country to country, being more common in warmer countries. 3. Oral CandidiasisOral thrush, a fungal disease of the oral mucosa and tongue, is caused most often by Candida albicans, although

Viral
1.Herpetic Keratitis- is caused by the same herpes simplex type 1 virus that causes cold sores and is latent in the trigeminal nerves. The disease is an infection of the cornea, often resulting in deep ulcers, that may be the most common cause of infectious blindness.

Protozoan
1.Acanthamoeba Keratitis- this amoeba has been found in fresh water, tap water, hot tubs, and soil. Most recent cases have been associated with the wearing of contact lense. Contributing factors are inadequate,unsani tary, or faulty disinfecting procedures, home-made saline solutions and wearing the contact lenses overnight and while swimming.

2. Hand, Foot and Mouth Disease (Coxsackie viral infection)- Hand, foot and mouth disease is a viral infection caused by a strain of Coxsackie virus. It causes a blisterlike rash that, as the name implies, involves the hands, feet and mouth. 3. Rubella (German

there have been reports of increased incidence of nonalbicans species. In the absence of other known causes of immune suppression, oral thrush in an adult is highly suggestive of HIV infection.

measles)- is an infection that primarily affects the skin and lymph nodes. It is caused by the rubella virus, which is usually transmitted by droplets from the nose or throat that others breathe in. It can also pass through a pregnant woman's bloodstream to infect her unborn child. 1.The drug trifluridine is often an effective treatment. 2. There is no specific treatment. Treatment is aimed at fever control and maintaining good oral hydration. 3. There is no specific treatment for Rubella; however, management is a matter of responding to symptoms to diminish discomfort. Treatment of newly born babies is focused on management of the complications. 1.Damage is often so severe as to require a corneal transplant, or even removal of the eye. Diagnosis is confirmed by the presence of trophozoites and cysts in stained scrapings of the cornea.

Treatment/ Prevention

1.Silver nitrate has been almost entirely replaced by antibiotics because of frequent coinfections by gonococci and sexually transmitted chlamydias, and silver nitrate is not effective against chlamydias. In parts of the world where the cost of antibiotics is prohibitive, a dilute solution of povidone-iodine has proven effective. 2.Tetracycline applied as an ophthalmic ointment is an effective

1. A presumptive diagnosis of fungal keratitis requires immediate empirical therapy. Natamycin ophthalmic suspension is the drug of choice for filamentous fungal infection. Fluconazole ophthalmic solution is recommended for Candida infection of the cornea. 2.Invasive sinusitis can progress rapidly, and typically necessitates surgery, often on a emergent basis and often requiring Amphotericin B

treatment. 3.Antibiotic ointments, especially tetracycline, are useful treatment. The disease can be controlled through sanitary practices and health education.

intravenously as well. There have been some forms of invasive sinusitis which can cause proptosis. There is a form of chronic invasive fungal sinusitis which is associated with visual abnormalities due to bony erosion from the ethmoids. 3. Oral candidiasis can be treated with topical anti-fungal drugs, such as nystatin, miconazole or amphotericin B. Topical therapy is given as an oral suspension which is washed around the mouth and then swallowed by the patient

Drawing/ Illustration

Chlamydia trachomatis causes Inclusion Conjunctivitis and Trachoma.

Fusariumcauses Fungal keratitis.

Person with Herpetic keratitis.

Eyes with Acanthamoeba keratitis.