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Alpha-galactosidase is a glycoside hydrolase enzyme that hydrolyses the terminal alphagalactosyl moieties from glycolipids and glycoproteins. It is encoded by the GLA gene.[1]
PSG: a new signal peptide prediction method N-region: length 7; pos.chg 1; neg.chg 1 H-region: length 9; peak value 6.06 PSG score: 1.66 von Heijne's method for signal seq. recognition GvH score (threshold: -2.1): -0.06 possible cleavage site: between 31 and 32
GvH:
>>> Seems to have a cleavable signal peptide (1 to 31) ALOM: Klein et al's method for TM region allocation Init position for calculation: 32 Tentative number of TMS(s) for the threshold 0.5: number of TMS(s) .. fixed PERIPHERAL Likelihood = 1.27 (at 281) ALOM score: 1.27 (number of TMSs: 0) MTOP: Prediction of membrane topology (Hartmann et al.) Center position for calculation: 15 Charge difference: -0.5 C( 1.0) - N( 1.5) N >= C: N-terminal side will be inside MITDISC: discrimination of mitochondrial targeting seq R content: 2 Hyd Moment(75): 2.26 Hyd Moment(95): 3.97 G content: 1 D/E content: 2 S/T content: 1 Score: -6.18 Gavel: prediction of cleavage sites for mitochondrial preseq R-2 motif at 27 LRF|LA NUCDISC: discrimination of nuclear localization signals pat4: none pat7: PVKRKLG (5) at 389 bipartite: none content of basic residues: 9.1% NLS Score: -0.04 KDEL: ER retention motif in the C-terminus: none ER Membrane Retention Signals: XXRR-like motif in the N-terminus: QLRN KKXX-like motif in the C-terminus: LKDL SKL: peroxisomal targeting signal in the C-terminus: none SKL2: 2nd peroxisomal targeting signal: none
VAC: possible vacuolar targeting motif: none RNA-binding motif: none Actinin-type actin-binding motif:
type 1: none type 2: none NMYR: N-myristoylation pattern : none Prenylation motif: none memYQRL: transport motif from cell surface to Golgi: none Tyrosines in the tail: none Dileucine motif in the tail: none checking 63 PROSITE DNA binding motifs: none none none
NNCN: Reinhardt's method for Cytplasmic/Nuclear discrimination Prediction: cytoplasmic Reliability: 94.1 COIL: Lupas's algorithm to detect coiled-coil regions total: 0 residues
>> prediction for QUERY is cyt (k=23) SeqName Potential Jury N-Glyc agreement result ---------------------------------------------------------------------Sequence 139 NKTC 0.6557 (9/9) ++ Sequence 192 NRTG 0.7524 (9/9) +++ Sequence 215 NYTE 0.7338 (9/9) ++ Sequence 408 NPTG 0.6258 (7/9) + WARNING: PRO-X1. Position
GvH:
>>> Seems to have no N-terminal signal peptide ALOM: Klein et al's method for TM region allocation Init position for calculation: 1 Tentative number of TMS(s) for the threshold 0.5: number of TMS(s) .. fixed PERIPHERAL Likelihood = 1.59 (at 551) ALOM score: 1.59 (number of TMSs: 0) MITDISC: discrimination of mitochondrial targeting seq R content: 2 Hyd Moment(75): 10.10 Hyd Moment(95): 8.81 G content: 0 D/E content: 2 S/T content: 0 Score: -4.35 Gavel: prediction of cleavage sites for mitochondrial preseq cleavage site motif not found NUCDISC: discrimination of nuclear localization signals pat4: RRRR (5) at 31 pat7: PDLRRER (3) at 3 pat7: PEKTRRR (4) at 27 bipartite: none content of basic residues: 11.1% NLS Score: 0.44 KDEL: ER retention motif in the C-terminus: none ER Membrane Retention Signals: KKXX-like motif in the C-terminus: LQSK SKL: peroxisomal targeting signal in the C-terminus: SKL SKL2: 2nd peroxisomal targeting signal: none
Actinin-type actin-binding motif: type 1: none type 2: none NMYR: N-myristoylation pattern : none Prenylation motif: none memYQRL: transport motif from cell surface to Golgi: none Tyrosines in the tail: none Dileucine motif in the tail: none checking 63 PROSITE DNA binding motifs: none none none
NNCN: Reinhardt's method for Cytplasmic/Nuclear discrimination Prediction: cytoplasmic Reliability: 89 COIL: Lupas's algorithm to detect coiled-coil regions total: 0 residues
GvH:
>>> Seems to have no N-terminal signal peptide ALOM: Klein et al's method for TM region allocation Init position for calculation: 1 Tentative number of TMS(s) for the threshold 0.5: INTEGRAL Likelihood =-10.88 Transmembrane 424 INTEGRAL Likelihood = -2.39 Transmembrane 457 INTEGRAL Likelihood = 0.26 Transmembrane 499 INTEGRAL Likelihood = -8.12 Transmembrane 539 INTEGRAL Likelihood =-12.42 Transmembrane 583 INTEGRAL Likelihood = -3.35 Transmembrane 626 INTEGRAL Likelihood = 0.16 Transmembrane 653 PERIPHERAL Likelihood = 3.66 (at 556) ALOM score: -12.42 (number of TMSs: 7) MTOP: Prediction of membrane topology (Hartmann et al.) Center position for calculation: 431 Charge difference: 3.5 C( 2.5) - N(-1.0) C > N: C-terminal side will be inside >>>Caution: Inconsistent mtop result with signal peptide >>> membrane topology: type 3b MITDISC: discrimination of mitochondrial targeting seq R content: 1 Hyd Moment(75): 1.57 Hyd Moment(95): 8.19 G content: 0 D/E content: 2 S/T content: 0 Score: -6.52 Gavel: prediction of cleavage sites for mitochondrial preseq R-2 motif at 12 MRP|AD NUCDISC: discrimination of nuclear localization signals pat4: none pat7: none bipartite: none content of basic residues: 9.0% NLS Score: -0.47 KDEL: ER retention motif in the C-terminus: none ER Membrane Retention Signals: XXRR-like motif in the N-terminus: RPAD none SKL: peroxisomal targeting signal in the C-terminus: none SKL2: 2nd peroxisomal targeting signal: none
VAC: possible vacuolar targeting motif: none RNA-binding motif: none Actinin-type actin-binding motif: type 1: none type 2: none NMYR: N-myristoylation pattern : none Prenylation motif: none memYQRL: transport motif from cell surface to Golgi: none Tyrosines in the tail: none Dileucine motif in the tail: none checking 63 PROSITE DNA binding motifs: none none none
NNCN: Reinhardt's method for Cytplasmic/Nuclear discrimination Prediction: cytoplasmic Reliability: 89 COIL: Lupas's algorithm to detect coiled-coil regions total: 0 residues
Potential Jury N-Glyc agreement result ---------------------------------------------------------------------Sequence 77 NISR 0.6659 (8/9) + Sequence 99 NLSK 0.7387 (9/9) ++ Sequence 113 NLTY 0.6722 (7/9) + Sequence 177 NETL 0.7619 (9/9) +++ Sequence 198 NGTK 0.6811 (9/9) ++ Sequence 302 NESS 0.5574 (7/9) + Sequence 715 NSTD 0.5137 (4/9) +
thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
PSG: a new signal peptide prediction method N-region: length 9; pos.chg 2; neg.chg 2 H-region: length 3; peak value -14.11 PSG score: -18.51 von Heijne's method for signal seq. recognition GvH score (threshold: -2.1): -13.71 possible cleavage site: between 44 and 45
GvH:
>>> Seems to have no N-terminal signal peptide ALOM: Klein et al's method for TM region allocation Init position for calculation: 1 Tentative number of TMS(s) for the threshold 0.5: number of TMS(s) .. fixed PERIPHERAL Likelihood = 1.43 (at 248) ALOM score: 1.43 (number of TMSs: 0) MITDISC: discrimination of mitochondrial targeting seq R content: 0 Hyd Moment(75): 7.67 Hyd Moment(95): 7.87 G content: 0 D/E content: 2 S/T content: 1 Score: -6.32 Gavel: prediction of cleavage sites for mitochondrial preseq cleavage site motif not found NUCDISC: discrimination of nuclear localization signals pat4: KRKR (5) at 25 pat4: RKRK (5) at 26 pat4: RRRK (5) at 144 pat7: PDGKRKR (5) at 22 bipartite: KRVAKRKLIEQNRERRR at 130 content of basic residues: 13.5% NLS Score: 1.56 KDEL: ER retention motif in the C-terminus: none ER Membrane Retention Signals: none SKL: peroxisomal targeting signal in the C-terminus: none SKL2: 2nd peroxisomal targeting signal: KITRNQCQL at 101 found
VAC: possible vacuolar targeting motif: none RNA-binding motif: none Actinin-type actin-binding motif: type 1: none type 2: none NMYR: N-myristoylation pattern : none Prenylation motif: none
memYQRL: transport motif from cell surface to Golgi: none Tyrosines in the tail: none Dileucine motif in the tail: none checking 63 PROSITE DNA binding motifs: Nuclear hormones receptors DNA-binding region signature (PS00031): *** found *** CVVCGDKATGYHYRCITCEGCKGFFRR at 53 checking 71 PROSITE ribosomal protein motifs: none none
NNCN: Reinhardt's method for Cytplasmic/Nuclear discrimination Prediction: nuclear Reliability: 89 COIL: Lupas's algorithm to detect coiled-coil regions 127 D 0.52 128 S 0.52 129 K 0.75 130 R 0.75 131 V 0.75 132 A 0.75 133 K 0.75 134 R 0.75 135 K 0.75 136 L 0.75 137 I 0.75 138 E 0.75 139 Q 0.75 140 N 0.75 141 R 0.75 142 E 0.75 143 R 0.75 144 R 0.75 145 R 0.75 146 K 0.75 147 E 0.75 148 E 0.75 149 M 0.75 150 I 0.75 151 R 0.75 152 S 0.75 153 L 0.75 154 Q 0.75 155 Q 0.75 156 R 0.75 total: 30 residues
4.3 %: endoplasmic reticulum 4.3 %: mitochondrial >> prediction for QUERY is nuc (k=23)