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Figure 1 1
What is the structure of individual modules? The structure of each of the repeat modules is very precise. The 33-35, normally 34 aa modules form two double -helixes. As we can see in figure 2, the first -helix also known as smaller or a -helix is form by aa 3 to 11. Residues 15 to 33 form the largeralso known as b -helix. Residues 12 and 13 are located in the loop joining both helixes
Marina Saiz Ala 08.03.12 together with a fixed Gly. As we can see in the figure this structure is curled due to van der Waals iterations between the helixes. Residue located at position 12 is the one in charged of stabilizing and maintaining this structure and allowing residue 13 to recognise DNA bases. To do so, the -helixes point away from DNA bases when they meet the DNA.
Figure 1 2
What is the structure of a tandem of at least 11.5 subunits? Previous individual module structure forming the - helixes will conform a right handed superhelix structure when joining DNA so DNA double strand can fit inside this super-helix. As we can see in figure 2, each 11 modules form a turn with the RVD in contact with the DNA so they can target it. Studies showed that when the super helix is not bound to DNA it has a pitch of 60 . However if the TAL bounds DNA the pitch will lower to 35 , nearly DNA major groove pitch where the TAL joins. In this super helical structure the small or a helixes will form the internal layer whether big or b - helixes will compose a more external layer.
Figure 1 3
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Page 717. Science Vol 335. February 2012. www.sciencemag.org. Solving the Structure of a Protein That Shows Promise as a DNA-Targeting Molecule for Gene Correction, Therapy. 2012. http://www.newswise.com/articles/
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