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Content 1. 2. 3. 4. Diluting Concentrated Acids (Last Login: 08/08/2009) Indicators (Last Login: 27/07/2009) Standard Buffer Solutions (Last Login: 27/07/2009) Special Solutions and Reagents (Last Login: 08/08/2009)
2. Indicators Indicators are substances which change from one color to another when the hydrogen ion concentration reaches a certain value, different for each indicator (1), and used to determine the specified end-point in a chemical reaction or to indicate that a desired change in pH has been effected (2). 2.1. Litmus: Digest 25g of litmus powder with three successive, 100-mL portions of boiling alcohol, continuing each extraction for about 1 hour. Filter, wash with alcohol, and discard the alcohol filtrate. Macerate the residue with about 25 mL of cold water for 4 hours, filter, and discard the filtrate. Digest the residue with 125 mL of boiling water for 1 hour, cool, and filter (2). 2.2. Methyl orange: Dissolve 1g of methyl orange in 1 liter of water. Filter if necessary (1). 2.3. Methyl red: Dissolve 100 mg of methyl red in 100mL of 95% ethyl alcohol. Filter if necessary (2). 2.4. Phenolphthalein: Dissolve 1g of phenolphthalein in 100mL of 95% ethyl alcohol (2).
Indicator
Litmus Methyl orange Methyl red Phenolphthalein
pH 4.2 4.4 4.6 4.8 5.0 5.2 5.4 5.6 5.8 5.8 6.0 6.2 6.4 6.6 6.8 7.0 7.2 7.4 7.6 7.8 8.0 8.0 8.2 8.4 8.6 8.8 9.0 9.2 9.4 9.6 9.8 10.0
Solution 1, mL Solution 2, mL Neutralized Phthalate Buffer 0.2 M Potassium 0.2 M NaOH Biphthalate 50 3.0 50 6.6 50 11.1 50 16.5 50 22.6 50 28.8 50 34.1 50 38.8 50 42.3 PBS (Phosphate Buffer Solution) 0.2 M Monobasic 0.2 M NaOH Potassium Phosphate 50 3.6 50 5.6 50 8.1 50 11.6 50 16.4 50 22.4 50 29.1 50 34.7 50 39.1 50 42.4 50 44.5 50 46.1 Alkaline Borate Buffer 0.2 M Boric Acid + 0.2 M NaOH Potassium Chloride 50 3.9 50 6.0 50 8.6 50 11.8 50 15.8 50 20.8 50 26.4 50 32.1 50 36.9 50 40.6 50 43.7
References
1. CRC Handbook of Chemistry and Physics. 63rd edition. Editor: R.C.Weast. CRC Press. 2. U.S. Pharmacopeia. USP 27 / NF 22.
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