Test-11 Topic:- Immunology, RDT & Carbohydrate metabolism 1) Restriction endonuclease cleaves DNA molecules at specific recognition sites.

One such enzyme has four recognition site on a circular DNA molecule. After complete digestion, how many fragments would be produced upon reaction with this enzyme? a) 4 b) 5 c) 3 d) 6 2) In ABC, angle A is larger than angle C and smaller than angle B by the same amount. If angle B is 670, angle C is a) 670 b) 530 c) 600 d) 570 3) Reverse Transcriptase has both ribonuclease and polymerase activities. Ribonuclease activity is required for a) The synthesis of new RNA strand b) The degradation of RNA strand c) The synthesis of new DNA strand d) The degradation of DNA strand 4) Toxic shock is caused by a) Toxins produced by some bacteria b) Excessive stimulation of a large proportion of T cells by bacterial super antigens c) Abnormal cytokine production by B cells d) Excessive production of immunoglobulins 5) For the half reactions shown below, which one of the following statements is true? E0 (V) O2 + 2H+ + 2e- H2O 0.815 O2 + 2H+ + 2e- H2O2 0.295 Cytochrome b (Fe3+) + e- Cytochrome b (Fe2+) 0.077 NAD+ + H+ + 2e- NADH -0.315 a) Oxygen is more easily reduced than NAD+ b) NAD+ has a lower tendency to get oxidized than oxygen c) Cytochrome b (Fe2+) is more easily oxidized than NADH d) Cytochrome b (Fe3+) is less easily reduced than oxygen 6) 1) A restriction enzyme AluI with a 4 bp recognition sequence produces restriction fragments that should have average size of a) 4096 bp b) 256 bp c) 16 bp d) 436 bp 7) Which of the following sequences along a double stranded DNA molecule may be recognized as a cutting site for particular restriction enzyme? a) AAGG

TTCC b) AGTC TCAG C) GGCC CCGG D) ACCA TGGT 8) The restriction endonuclease Eco521 recognizes the sequence C/GGCGG. DNA cut by which of the following enzymes could be cloned into a plasmid digested with Eco521? a) EcoRi G/AATTC b) XmaIII C/GGCGG c) SmaI CCC/GGG d) SacII CCGC/GG 9) The substrate for restriction enzyme is a) ssRNA b) partially double stranded RNA c) Cell wall proteins d) dsDNA 10) One molecule of secretory IgA can at most bind to a) 10 molecules of antigen b) 4 molecules of antigen c) 2 molecules of antigen d) 1 molecule of antigen 11) HAT medium is used for selecing the hybridomas based on the following P. Only hybridoma will grow since it inherited HGPRT genes from B cells and can synthesize DNA from hypoxanthine Q. Myeloma cells will not grow in cultures since de novo synthesis is blocked by aminopterine and due to the lack of HGPRT enzyme a) Only P is true b) Only Q is true c) Both P and Q are true d) P is tre nad Q is false 12) Which compound cannot cross the inner membrane of mitochondria? a) ATP b) Pyrophosphate c) Pyruvate d) Acetyl CoA 13) A researcher has isolated a restriction endonuclease that cleaves at only one specific 10 base pair site a) Would this enzyme be useful in protecting cells from viral infections, given that a typical viral genome is 5 X 104 bp long? b) Restriction endonuclease is slow enzymes with turnover number of 1 per second. Suppose the isolated endonuclease was faster with turnover numbers similar to those for carbonic anhydrase (106 per second). Would this increased rate be beneficial to host cells, assuming that the fast enzymes have similar levels of specificity?

The correct combination of answer is 1) (A) No (B) Yes 2) (A) No (B) No 3) (A) Yes (B) No 4) (A) Yes (B) Yes 14) The presence of IgA in the lumen of mucosa is due to which process? a) Phagocytosis b) Pinocytosis c) Transcytosis d) Endocytosis 15) The enzyme responsible for somatic hypermutation is a) AID b) RAG c) Telomerase d) DNA polymerases and exonucleases 16) Which statement is not true of PFK 1? a) Activated by AMP b) Inhibited by Citrate c) Inhibited by ATP d) Inhibited by Fructose-2, 6- bisphosphate 17) Which one of the following statements concerning the organization of Ig genes is correct? a) V and J regions of embryonic DNA have already undergone a rearrangement b) Light chain genes undergo further rearrangement after surface IgM is expressed c) VH gene segments can rearrange with J kappa and J lambda segments d) VDJ segments coding for Ig VH region may associate with different heavy chain constant region genes 18) A circular piece with maximum possible area is cut out from a square cloth of side 14cm. What is the area of the remaining portion? (consider value of to be 22/7) a) 42 b) 196 c) 21 d) 154 19) What is the role of hypoxanthine in HAT medium used for hybridoma generation? a) It is an antibiotic b) It blocks De novo DNA synthesis c) It is used for negatively selecting myeloid cells d) It is used for negatively selecting B cells 20) Human genome contains 3 10 9 bp having all four bp in equal proportion. A restriction endonuclease can cut a segment of 4 bp long. Then how many fragments will be obtained after digestion(a) 1.2 10^7 (b) 1.2 10^6 (c) 1.2 10^5 (d) 1.2 10^4

Topic:- cumulative, Proteins, Membranes, Microbial physiology, Lipids

1. A bacterial culture (200microL containing 1.8x109

cells) was treated with an antibiotic Z (50micro g/ml) for 4h at 37C. After this treatment , the culture was divided into two equal aliquots. Set A: 100l was plated on Luria agar Set B: 100l was centrifuged, the cell pellet washed and plated on Luria agar. After incubating these two plates for 24 hrs at 37C Set A plate showed no colonies, whereas the Set B plate showed 0.9x109 cells. This experiment showed that the antibiotic Z is a) Bacteriostatic b) Bacteriocidal c) Bacteriolytic d) Apoptotic 2. The nutritional medium was supplied to growing bacteria. If whole nutrient gets depleted in 20 cycles, then stage at which the amount of nutrient was half used a) At the end of 10 cycle b) At the beginning of 19 cycle c) At the end of 19 cycle d) At the end of 11 cycle 3. Mutations in the genes encoding ------ proteins contribute to several genetic diseases, including cystic fibrosis a) any transporter b) active transporter c) ABC transporter d) Flippases and Floppases e) Uniport 4. A person who was starving since morning has his lunch in the afternoon and goes for work. After his working hrs he plays basket ball for an hour. Now he starts feeling hungry again. Where do you expect to find the GLUT 4 transporter in the morning, afternoon and evening. a) Will be part of membrane, then endosome and membrane again b) Will be part of endosome, membrane and remain in membrane c) Will be part of endosome, membrane and then endocytose d) Will be a part of membrane always 5. Use this typical bacterial growth curve to answer the following question.

b) Increase the production of lipid intracellular signaling molecules c) Stabilize membrane proteins d) Increase permeability to water 12. The direction of migration of the tripeptide Lys-Lys-Lys in an electric field at pH=3 and at pH=10 is a) Cathode and cathode respectively b) Cathode and Anode respectively c) Anode and cathode respectively d) Anode and Anode respectively 13. The amino acid carboxyproline has three ionizable groups with the following properties Alpha carboxyl- pKa 2.0 Alpha amino- pKa 10.5 Gamma carboxyl- pKa 3.8 The isoelectric point for this amino acid is a) 2.9 b) 6.25 c) 7.15 d) 5.4 14. Telomerase is composed of a) Only protein b) Only RNA c) Protein and RNA d) Protein and DNA 15. A protein segment consists of a stretch of 10 residues that adopt an alpha helical secondary structure conformation. What is the maximum number of hydrogen bonds that can stabilize this alpha helix? a) 4 b) 5 c) 6 d) 7

Which section shows a growth phase where the number of cells dying equals the number of cells dividing? a) A b) B c) C d) D e) A and C 6. Which of the following is used to grow bacterial cultures continuously? a) Chemostat b) Coulter counter c) Hemostat d) Petroff-Hausser Chamber 7. The term aerotolerant anaerobe refers to an organism a) that doesn't use oxygen but can grow in the presence oxygen. b) is killed by oxygen. c) uses oxygen when present or grows without oxygen when absent. d) requires less oxygen than is present in air. e) prefers to grow without oxygen. 8. If you started with 1000 E.coli with a generation time 30 mins, how many bacteria would you have after 3 hrs? a) 320 b) 640 c) 6400 d) 64000 9. A bacteria divides every 20min. After 4hrs of incubation flask contained 2x10-6 bacteria. At what time media will contain 1x10-6 bacteria. a) 273min b) 512min c) 239min d) 726min 10. 1gm of cell culture contains 10^9cells and occupies 1ml. If each cell contains 6.4x09bp. What is that amount of DNA present in cell a) 4.224x10^8 b) 6.228x10^8 c) 3.447x10^6 d) 4.224x10^6 11. At low temperature the sterol content of membrane increases in cultured cells in order to a) Increase membrane fluidity