Application Form 1. 2. 3. 5. Full Name: ___________________________________________ Designation: __________________________________________ Sex: ____________ Present address: ______________________________________________________ ___________________________________________________ 6. 7. 8. Tel No.

__________________ (office )_________________(Res ) Email address _________________________________________ 4. Date of birth ____________________

A Training Course on Molecular Diagnostics & Bioinformatic Tools From 8th to 28th November, 2011

Teaching/ research /professional experience along with the posts held (during last five years) Post held Institution Period Nature of duty

Course Director: Head of the Department Course Coordinator: Dr. M.L. Sangwan Course Faculty: Drs. Trilok Nanda, Minakshi, & Aman Kumar

9.

Academic records Subjects Year of passing Percent/ OGPA University

Exam Passed

Ph.D. Masters degree Bachelors degree

Signature of the applicant

Organised by 10. Date: ________________ Place: _____________________

11. Recommendation of forwarding institution/organisation:

Department of Animal Biotechnology College of Veterinary Sciences Lala Lajpat Rai University of Vety. & Animal Sciences Hisar 125 004, Haryana

Hisar: It is located 165 Km from Delhi, 320 Km from Jaipur. It is connected from Delhi by train as well as bus. The buses ply between interstate bus terminus (ISBT) New Delhi and Hisar. There are two trains from Delhi viz., Haryana Express (leaves New Delhi Railway station at 6:00PM) and Kishan Express (leaves Old Delhi railway state at 2:40PM). During month of Nov. the temp will be 16 to 20 C. Duration: 8th November to 28th November, 2011 Course Fees: Participants are requested to pay a sum of Rs. 5000 (Rs five thousand) as registration fee. The registration fee shall be deposited in cash at the time of registration on November 8, 2011. Laboratory and computing facilities: The laboratories are well equipped with modern equipments and other lab wares. Accommodation: Arrangements for the stay of the participants during the training program will be made in faculty house of the university on the payment basis. Number of participants: The maximum number of participants shall not exceed 20. Participants and eligibility: Participants are invited from ICAR Institutes/ SAU/Basic Science Institutes/ Sate Governments/Private Organizations. They should be graduates having interest in the training. How to apply: The application for participation may be sent in prescribed format, duly forwarded by head of the institution. It should reach to the Course Director latest by November 1st, 2011 by post, fax or by email .TA & DA of the participants will be borne by participants/sponsoring institutions/ organisations etc. The participants will also have to pay for their boarding and lodging charges during the training program. The organizers of the course will not bear any expenses on account of the participants. The participants will be informed about their selection by November 4th, 2011.

A Practical training course on Molecular Diagnostics and bioinformatic tools Objective: The science of diagnostics gets revolutionized with the development of Molecular biology tools as it provides high level of sensitivity and specificity to the diagnostics. These tools can detect various pathogenic organisms like bacteria, viruses, etc. through the presence of DNA, RNA or protein sequences in clinical samples. Nowadays commercial kits are available to detect pathogens directly from clinical samples and make it possible to obtain results in just a few minutes or hour, but most of these kits are exogenous in origin and thus very much expensive. Therefore, it is important that highly specific diagnostic tests should be developed indigenously based on the Indian strains of pathogens. Hence, the present practical training course is designed to provide hands on training on basic molecular biology techniques used for diagnosis of pathogens in blood, semen, clinical tissues, faecal samples and characterization of pathogens and breeds of animals. For better understanding of data of molecular biology, it is essential to have the basic knowledge of bioinformatic tools; keeping these points in view this training programme will also include hands on training on bioinformatics tools. Course contents: Preparation of reagents, buffer and media. Extraction and purification of nucleic acid of viral agents from various sources like blood, semen, clinical tissues, faecal samples, etc. Electrophoretic separation of nucleic acid and protein. RNA-Polyacrylamide gel electrophoresis for segmented viruses. Template preparation for Polymerase chain reaction (PCR) Amplification of gene of interest using conventional PCR Synthesis of cDNA and RT-PCR based amplification of viral genes. DNA markers like RAPD,RFLP etc. Molecular cloning of genes in suitable vectors Sequence alignment tools e.g. BLAST Designing of PCR Primer Analysis of nucleotide sequences.

All correspondence may please be addressed to:

Dr. M.L. Sangwan, Course Coordinator Department of Animal Biotechnology, COVS LLRUVAS, Hisar
Phone no. 01662- 289132 (office) 01662- 244384 (Res), Cell: 09896588704 Fax 01662-234613, 234952

Email: abt@hau.ernet.in,

mlsangwan@hau.ernet.in

Photocopy of the application form can be used Information can also see on website (www.hau.ernet.in)

Phylogenetic analysis of nucleic acid sequence data.