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AmProtein Expression Technology Technical notes AmProtein Current Perfusion Bioreactor Technical notes
AmProtein Current Suspension Bioreactor Technical notes AmProtein Current Mini-bioreactors Bioreactor
Supporting Products
Overview Cell Line Development Service Bioassay Process Development and CMC Service Vaccine Production
Culture Media Generics and Modified Generics

Vaccine Production

Using our paper-carrier based "Current" perfusion culture bioreactor, we are able to culture cells at extremely
high densities, replacing classical roller bottle and current microcarrier systems. Because of this fact, we can use
our "Current" perfusion bioreactor to culture human and animal vaccine producing cells (such as VERO MDCK,
etc...) very rapidly to counter global disease outbreaks. Furthermore, we have developed a scalable seeding
process that has simplified the trypsin-digest/seeding process. We have tested cells from several species (see
data below) with remarkable results, improving the industrial manufacture of vaccines.

"Current" Paper­carrier Perfusion Bioreactor Cell Culture Data 

(cell density/gram paper-carrier by paper-carrier total weight) for vaccine production

5L perfusion bioreactor 50L perfusion bioreactor 150L perfusion bioreactor

Cell line
(cell#/gram×paper-carrier (cell#/gram×paper-carrier (cell#/gram×paper-carrier
name
total weight 150 grams) total weight 1200 grams) total weight 3600 grams)

VERO
6.0±1.2×108cells/g×150g 6.5±2.1×108cells/g×1200g Pending
(human)

MDCK
5.0±1.0×108cells/g×150g Pending Pending
(human)

2BS
3.0×108cells/g×150g Pending Pending
(diploid)

Mark145
3.5±0.8×108cells/g×150g Pending Pending
(animal)

ST1
4.0±1.0×108cells/g×150g Pending Pending
(animal)
 DF-1
2.5±0.3×108cells/g×150g Pending Pending
(chicken)

CIK (fish) 1.0±0.2×108cells/g×150g Pending Pending

EPC (fish) 1.2±0.3×108cells/g×150g Pending Pending

Conclusions:

1) A 50L paper-carrier perfusion bioreactor equals a production plant of 1200 large roller bottles and is perfect for
larger-scale animal and human vaccine production, including chicken and fish. It is also great for rapid vaccine
production of a deadly virus outbreak (such as SARS and bird flu outbreaks) after the virus DNA extracted.
2) Single-use and low cost.

Advantages:

1) Due to extremely high cell density, cell-cell growth support reaches the peak greatly for low-growth rate
vaccine production cell lines;

2) Compared with roller-bottles and micro-carrier methods, seeding method (cell wash & trypsin-digest) for scale
up is greatly simplified.

3) DO,pH,temperature control and cell line-specific medium optimization.

Human & Animal Vaccine Process Development

Taking advantage of AmProtein's high-density cell culture perfusion system we have developed MDCK, VERO,
Chicken and Fish cell process. These processes are for sale together with the perfusion system to interested
vaccine manufacturers.

AmProtein also welcomes collaborations to work on newly discovered vaccines and push them towards
commercialization or grant applications towards final commercialization.
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