Annual Report 2010-2011

Centre for Advanced Research in Sciences (CARS), University of Dhaka Director: Professor Dr. Altaf Hussain UGC Professor: Professor Dr. Ajoy K. Roy Principal Scientist: 02
1. Dr. Gazi Nurun Nahar Sultana 2. Dr. Latiful Bari Executive Engineer: M. Abdul Maleque Senior Scientist: 01 Dr. M. Zakir Sultan Post Doctoral Research Fellow: 02

1. Dr. Rumana Akhtar Jahan 2. Dr. M. Saleh Ahmmad

Research Fellow: 04 1. Ms. Rokeya Begum 2. Ms. Hasina Akhtar Shimul 3. Ms. Asma Rahman 4. Mr. Arafat Rahman Account Mr. Monir Hossain Assistant Engineer: 1. Fazlul Haque 2. M. Humayun Kabir Raju Store Officer: M. Ruhul Amin Administrative Officer: Ms. Safi Rezwana Technical Assistant: 1. Md. Ashraful Islam 2. M. Mafizur Rahman Other Stuff: 16 Annual Revenue Budget Tk: 60, 00,000/ (Sixty-lakh taka only) Executive:

Established: 2004 The Centre is in operation at present with ten (11) equipped laboratories. Each of the floor is having 10000 sft area. The fifth and sixth floor of the Centre is now in full operation with three more advanced R&D laboratories (Material Science, Food Analysis, and Pilot Plant Research Laboratory and Tissue Culture Laboratory). The second floor of the Centre is having one six well-furnished guest rooms and a cafeteria. This cafeteria is open only for the stuffs of the Dhaka University for lunch. First floor of the Centre is having one conference room with 90 seats and is available for the workshop, conference and seminars on payment basis. There is one committee room in the ground floor of the centre with 30 seats. 500 KVA generator for alternate electricity supply supports the electricity of the Centre during power failure

The Centre now has the following 11 Laboratories:

Basic Equipment Laboratory: Gas-Chromatograph, Scanning Electron Microscope,

Ultracentrifuge, Digital Polarimeter , Moisture Analyzer, Elemental Analyzer CHN/S/O & Total Organic Carbon (TOC) Analyzer with Gas Generator, Single and, Ultra Low Temp freezer, Rotary Evaporator, Autoclave, Ice Maker, Bio-hood, Double
distillation plant, Nano-pure water system, Laminar Flow, Ultrasonicator, pH meter, Vortex mixer, Analytical balance, Top loading balance, Single beam UV-VIS spectrophotometer, Water bath, Drying oven.

Drug Analysis & Research laboratory: Flame photometer, Differential Scanning

Calorimeter (DSC), Digital Melting Point Apparatus, Thermostatic Water Bath, Ultrasonic Bath, Conductivity meter, Analytical Balance (Digital) Glass vacuum filtration system, Hot air sterilizer, Refrigerated centrifuge (Bench top), Viscometer, pH meter, Trinocular Microscope with camera, Programmable Friability Tester, Programmable Spectrometer,

Tap

Density

Tester,

Hardness

Tester,

Stability

Chamber:

Pharmaceutical QC equipment, Cooled Incubator, Colony Counter, Fluorescence HPLC System, Various Tablet Dissolution Testing Equipment, Disintegration Testing Equipment, Friability Testing Equipment.
Environmental and Analytical Research Laboratory: Automated Atomic Absorption Spectrophotometer, Suppressor Type Ion Chromatograph (both cation and anion), PC Based Thermo gravimetric Analyzer, Microwave Digestion System, Muffle Furnace, Automated Sample dilution System, HPLC with PDA detector. FT-IR Spectrophotometer,

Double Beam Recording Spectrophotometer, Moisture analyzer.

Food Analysis Research laboratory: An automated HPLC with RI / UV detectors, An

FT-NIR spectrometer, and Microbiological growth system, Stomacher, Fat and Protein Analyzer.
Genetic Engineering and Biotechnology Research Laboratory: Automated ABI Prism 3130 Genetic Analyzer, Horizontal Gel Electrophoresis system, Thermal Cycler (Bio-Rad and Eppendroff), Gel Documentation system, Laboratory Oven, pH Meter, Mini spinner, Centrifuge (Eppendroff), Vortex Mixer, Analytical Digital Balance, Heating Block, Laboratory Autoclave, Cooled Incubator Shaker, Laminar Air Flow Cabinet, Laboratory Incubator, Hybridization Incubator, Dry Heat Sterilizer, Minus 40oC Freezer, High Speed Refrigerated Centrifuge, Vertical Electrophoresis system, Multichannel Auto Micropipette

Material Science Research Laboratory: Scanning potentiometer, Impedance analyzer, electrodes, and accessories, clean bench. Non-Linear Optics and Laser Spectroscopy Research laboratory: Quantum Ray Nd:

YAG Laser, Monochromator, mounted on an Optical breadboard, Air Cooled Argon Ion Laser, Fiber coupled Laser system, OPO with pump Laser system, Director and
Spectrograph with Accessories, Digital Oscilloscope, Analog Oscilloscope, Delay

Generator, HV power supply, Optical Detectors and Optical Components, Optical Table
including air mounted legs, Optical breadboard, Peltier-cooled gated ICCD with

computerized spectrometer, Chiller for supplying cooling water to various high-power laser systems.
Pilot Plant Research Laboratory: Bioreactor (5 L), Bioreactor (10 L), Bioreactor (100

L), Freeze dryer, Autoclave, Centrifuge, Incubator, Orbital Shaker, Clean Bench, Peristaltic Tubing Pump
Preparation Laboratory: This laboratory has the facilities for sample processing. Software Development and Research Laboratory: 3 modern servers, Sun/Sun fire model, 5 work stations (IBM) with other supporting facilities. Psychology Assessment and Management Laboratory: Psychological Test materials.

Research / Activities: 1. DNA Sequencing Laboratory/Genetic Engineering and Biotechnology Research Laboratory: (Started in 2006): In Charge: Dr. Gazi Nurun Nahar Sultana.
Running projects:

1. Study on -thalassemia gene screening for diagnosis of high frequency single gene disorder
among population of Bangladesh. PI: Dr. Gazi Nurun Nahar Sultana, Principal Scientist, CARS, DU This project is supported by TWAS funding and is about to complete the first phase research. Amount received Tk: 15, 00,000/

2. Possible association of frequent mutations in the mitochondrial DNA (mtDNA) for early
diagnosis of primary breast cancer: Case-control study. Principal Investigator: Dr. Gazi Nurun Nahar Sultana, Principal Scientist, CARS, DU Co-Investigator: Professor Dr. Mizanur Rahman, Dept. Surgical Oncology, National Institute of Cancer Research and Hospital, Dhaka. Co-Investigator: Dr. Tasmia Tahmid, Breast Surgeon, Depharm Diagnostic Centre, Dhanmondi, Dhaka. CARS, DU., support this project.

3. Environmental Oxidative Stress on Mitochondrial DNA mutations in fish: Possible role in

Ageing, Reproductive performance and Mortality. PI: Professor Dr. Kawser Ahmed, Dept of Fisheries, DU Co-PI: Dr. Gazi Nurun Nahar Sultana, Principal Scientist, CARS, DU Funding: Ministry of Science and Information & communication Technology Amount received Tk: 5, 00,000/ New Instrument Procured: 1. One Thermocycler; funding from TWAS 2. One Mini High Speed Centrifuge; funding from TWAS 3. Nano-Drop Spectrophotometer; funding from CARS MS/M. Phil/Ph.D. Students: 06 students have completed MS thesis projects, summer trainee 03, M.Phil/PhD student 02. Thesis Submitted:

(i)

A comparison study of mtDNA polymorphisms correlated with the risk of breast cancer in Bangladeshi Population

PI: Dr. Gazi Nurun Nahar Sultana, Centre for Advanced Research in Sciences (CARS), DU. Supervisor: Dr. Jesmin, Dept of Genetic Engineering and Biotechnology, DU (ii) Human mtDNA Haplogroup in Bangladesh: Dissecting the Phylogenetic Tree of General Population of Bangladesh specific Lineages. PI: Dr. Gazi Nurun Nahar Sultana, Centre for Advanced Research in Sciences (CARS), DU. Supervisor: Professor Anwarul Azim Akondh, of Genetic Engineering and Biotechnology, DU (iii) A Profile of Atrial Septal Disease in Patients Undergoing Surgical Repair, including the Frequencies of Different Morphological Types and NKX2.5 Gene Mutation.

Supervisor: Professor K. M. Shamim, Chairman, Dept of Anatomy, BSMMU. Co-Supervisor: Gazi Nurun Nahar Sultana, Centre for Advanced Research in Sciences (CARS), DU.
2. Environmental and Analytical Chemistry Research Laboratory: In Charge: Dr. Gazi Nurun Nahar Sultana MS students from Chemistry, Biochemistry and Molecular Biology, Institute of Food and Nutrition, Dept. of Zoology, Dept. of Botany have completed their thesis work in collaboration with CARS, DU. Projects: 1. Determination of micronutrients and water-soluble vitamins in fish samples collected from market. PI: Gazi Nurun Nahar Sultana, Research Fellow: Mrs. Hasina Akhter Shimul Thesis Submitted:

1. Ms. Tohora Khanom, M.Sc. student from the Department of Biochemistry and Molecular
Biology. Thesis titled Contamination of Chromium in food chain from tannery solid waste.

3. Food Laboratory: In Charge: Dr. M. Latiful Bari

Projects received :( National) 1) Ministry of Information & Science and technology R& D research grant 2010-2011

project entitled Systematic analysis of Salmonella in poultry of Bangladesh; Its Seroprevalence, Drug Resistance and Zoonoses funded 8,00,000 Taka.
Collaborative project :( International) 1). JSPS Grant-in-aid for scientific research 2011 projects entitled Developing an evidencebased effective and applicable method to increase hygiene condition of vegetable farms in developing countries. Funded for 3 years (FY2011-2013) and grant total 5,000,000 Yen. Member countries: Yasuhiro Inatsu (National Food Research Institute, NARO, Japan) Md. Latiful Bari (CARS, University of Dhaka, Bangladesh) Borarin Buntong (Royal University of Agriculture, Cambodia) Thongsavath Chanthasombath (Clean Agriculture Development Centre, Lao PDR)

On-going Research: (National)

1. Collaboration research

entitled Salmonellosis in Poultry of Bangladesh: Its Seroprevalence, Drug resistance and Zoonoses-with Professor M. Anwar Hossain, Department of Microbiology, University of Dhaka. 2. Collaboration Research entitled Effectiveness of ohomic current and UV light on reducing microorganisms in water- with Professor G. Rabbani, Department of Biomedical Physics, University of Dhaka. 3. Collaboration research entitled Effectiveness of novel sanitizer in reducing pathogenic microorganisms in fresh cut market vegetables.-with Professor M. Anwar Hossain, Department of Microbiology, University of Dhaka.

On-going research (international)

1. Working on a research project entitled ensuring food safety from farm to table 2008-2013,
funded by MAFF.

2. Application of USST in eliminating foodborne pathogen and mycotoxins in food grains.

New innovative research jointly conducting by JSP Co. Ltd, Saitama, Japan and Food Analysis Research Laboratory, CARS, along with IFRB of Bangladesh Atomic Energy Commission. MS/M.Phil Thesis Submitted:

1) Ms. Salma Akther, M.Sc. student from the Department of Microbiology. Thesis titled
Effectiveness of sanitizers in reducing natural microflora and pathogenic microorganisms in salad vegetables. 2) Ms. Fauzia Ahmed Rupa. M.Sc. student from the Department of Microbiology. Thesis titled Evaluation of Inoculation Method and the efficiency of sanitizer in reducing Escherichia

coli O157:H7, Salmonella, and Listeria monocytogenes Inoculated on the Surface of Tomatoes. 3) Mr. Sumon Barui, M.Sc student from the Department of Genetic Engineering and
Biotechnology. Thesis titled determination of protein and fat of liver, whole-brain, kidney and other organ of mice after arsenic ingestion. 4) Md. Showkat Mahmud, Ph.D. student from the Department of Microbiology and working on Prevalence of Salmonella Serovars and Antimicrobial Resistance Profiles in Poultry of Bangladesh. 4. Drug Research Laboratory: In charge: Dr. M. Zakir Sultan Running Project

1. Quality assessment of some commonly used antimicrobial preparations. 2. Biotransformation and bioequivalence studies of quinolone antibiotics in Bangladeshi Bangladeshi volunteers. 3. Development and validation of analytical method of tiemonium methylsulphate (drug used in visceral pain). 4. Drug-excipients compatibility study. 5. In vitro and in vivo study of drug-drug interactions. 6. In vitro and in vivo study of drug-food, drug-fruit, and drug-vegetable interactions. MS Thesis submitted: HPLC 1. Anjan Kumar Roy (Researcher, Institute of Nutrition and Food Sciences)

2. M. Mohiuddin (M. Sc Thesis student, Supervisor: Department of Biochemistry and Molecular Biology)

Prof. Dr. Yearul Kabir,

3. Helal Morshed (M. Pharm Thesis, Supervisor: Prof. Dr. Md. Amjad Hossain, Department of Pharmaceutical Chemistry) 4. Gazi Md. Zia-Ul-Huda (M. Pharm Thesis student, Supervisor: Prof. Dr. Md. Abdur Rashid, Department of Pharmaceutical Chemistry)

5. Non-Linear Optics and Laser Spectroscopy Research laboratory: In charge: Prof. Dr. AFM Yusuf Haider. ??? Need Information from Yusuf sir Training Program:

1. Conducted three weeks of Food Microbiology training course for the Laboratory
Analysts (June 25 July 13) 2011, organized by Institute of Chemists and Chemical Technologists, Bangladesh (ICCTB), in collaboration with the Department of Chemistry & Centre for Advanced Research in Sciences (CARS), University of Dhaka (DU), Dhaka 1000, Bangladesh. 2. Conducted one week training on Analysis of food elements by Atomic Absorption Spectrophotometer (AAS) for the Laboratory Analysts (June 25 July 13) 2011, organized by Institute of Chemists and Chemical Technologists, Bangladesh (ICCTB), in collaboration with the Department of Chemistry & Centre for Advanced Research in Sciences (CARS), University of Dhaka (DU), Dhaka 1000, Bangladesh.

3.
Seminar/symposium Organizer:

1) The 5th International Conference on Microbiology, Health and Environment was held in
Dhaka University on Dec 26-28, 2010 and was organized by DU, BSM, BAS and BJAST. 2) CARS and OMC have organized seminar on Technical support of Scanning Electron Microscope (SEM) jointly on December 2010. 3) Seminar on Food Microbiology by Dr. Latiful Bari February 2011 4) Seminar on Breast Cancer and SNPs of mtDNA in women of Bangladesh by Dr. Gazi Nurun Nahar Sultana, June 2011 5) Seminar on Carbon to drug design by invited scientist from UK in December 2010 More seminars information need to add Publication:

1. Gazi Nurun Nahar Sultana, Atiqur Rahman, M. Manjurul Karim, ADA Shahinuzzaman, Rokeya Begum and Rowsan Ara Begum, Breast cancer risk associated mitochondrial NADH-dehydrogenase subunit-3 (ND3) polymorphism. Journal of Medical Genetics and Genomics, 2011, 3, 131-135. 2. Gazi Nurun Nahar Sultana, Zakaria Shamim, Hosne Ara Begum, Rokeya Begum, Atiqur Rahman, ADA Shahinuzzaman, M. Anwar Hossain, Advancement of Molecular Biology and Biotechnology in Bangladesh, A book Published by

Asiatic Society of Bangladesh, Title: Advancement of Science in Bangladesh, Chapter-5, 2011. 3. M. L. Bari, Y. Sabina, K. Isshiki and S. Kawamoto. (2011) Chapter 9. Microbiological Safety and Quality of Processed Shrimps and Fishes, In book entitled Aquaculture Microbiology and Biotechnology; Volume 2 Didier Montet and R.C.Ray (Editors), Science Publisher, Inc, NH, USA. ISBN 978-1-57808-711-2; May 2011 4. Hossain Uddin Shekhar, Masao Goto, Jun Watanabe, Ichiho Konishide-Mikami,
Md. Latiful Bari and Yuko Takano-Ishikawa. (2011). Multi food functionalities of Kalmi Shak (Ipomoea aquatic) grown in Bangladesh. Agriculture, Food and Analytical Bacteriology. Vol 1(1): 1-9. 5. M Mahfuzul Hoquer, Shemona Rattilar, M Asaduzzaman Shishirr, M L Bari, Y Inatsu, and S Kawamoto (2011). Antibacterial Activity of Bthanol Extract of Betel Leaf (Piper betle L.) Against Some Food Borne Pathogens. Bangladesh J Microbiol, Volume 28 (2); pp 58-63. 6. Md. Latiful BARI1*, Katsuyoshi ENOMOTO, Daisuke NEI, and Shinichi KAWAMOTO (2011). Development of Effective Seed Decontamination Technology to Inactivate Pathogens on Mung Bean Seeds and Its Practical Application in Japan. JIRCAS Agricultural Research Quarterly (JARQ). 45( 2) : 153-161. 7. Rachel Ramos Elano, Tomoko Kitagawa, Md. Latiful Bari, Susumu Kawasaki, Shinichi Kawamoto, Yasuhiro Inatsu. (2010). Comparison of the Effectiveness of Acidified Sodium Chlorite and Sodium Hypochlorite in Reducing Escherichia coli. Foodborne Pathogens and Disease. Vol. 7(12): 1481-1489. doi:10.1089/fpd.2010.0595. 8. Nei D, Bari M. L, S.Todoriki, Y. Inatsu and S. Kawamoto (2010). Combined Effect of Low-Dose Irradiation and Acidified Sodium Chlorite Washing on E. coli O157:H7 Inoculated on Mung Bean Seeds. Foodborne Pathogen Dis. Vol 7(10): 1217-1223.

9. Stability assessment of cephradine suspension formulated in Bangladesh. Journal of Scientific Research, 3(2), 383-391, 2011. 10. In vitro transdermal delivery of metformin from a HPMC/ PVA based TDSpatch at different pH. Journal of Scientific Research; 3 (3), 651-657 (2011). 11. Preliminary cytotoxicity and antimicrobial investigation of Anthocephalus chinensis, Journal of Scientific Research, Journal of Scientific Research, 3 (3), 689692 (2011).
Service provided:

1. HID analysis method developed at the DNA sequencing laboratory and 22 samples have
been analyzed for immigration/paternity test/siblings. The service on payment basis is open for all.

2. 300 Samples have been sequenced for CARS as well as; (i) (ii)
(iii) (iv) (v) (vi) (vii) samples of the external clients on payment basis samples for the thesis students from Centre and other Departments of DU Nano drop facility is also given to different Faculties Gel Documentation/PCR/Centrifuge services are also supported HPLC service to faculties and External clients FT-IR service to faculties and external clients TGA, SEM service to faculties and external clients

(viii)

Approx. 1500 samples have been analyzed on payment by AAS. (Number of samples can be asked to Mr. Monir)

Online Library Materials: Journal can be accessed through AGORA, HINARI, OARE and Dhaka University E-resources. Through these access about 9-10 thousands of journals can be read and downloaded. Centre has initiated a Postdoctoral Fellowship program for supporting research activities.