Acknowledgement

In the completion of the present work there are feeling of achievement and satisfaction in moment of happiness I take opportunity to all those who mattered. I am highly grateful to Dr. W.S.Lakra, director of the National Breau of Fish Genetic Resources, Lucknow , for giving permission to undergo a short term summer training program from 1st june 2011 to 30th june 2011 at this institution . I also thank Dr. L.K.Tyagi , scientist ,Sr. scale & incharge training & placement cell for considering me as a summer trainee and allotted concerned scientist. I am highly thankful to respected Dr.B.Khushwaha , Sr.scientist ,cytogenetics and genotoxicity laboratory ,NBFGR, under whom I felt blessed to work. I am also very greatful to Dr. N.S.Nagpure, principal scientist and the head of molecular biology and biotechnology division and Dr.Rabindra kumar,Sr. scientist, cytogenetics and genotoxicity laboratory , not only for his constant encouragement and suggestion but also showing me the path of dedication and patience. He guided me the way for a scientific approach through which I came across. I siencerely thanks to Mrs. Rashmi Srivastava(SRF) ,Mrs. Pratibha singh, Ms.Richa who have helped me to complete my work. I want to mention Mr.Rajesh sir ,without his assistance it would be impossible to complete this piece of work. At last , I also persent gratitude to my parents who have encouraged me at each piece of work and every step of my life and because of whom I learnt so much in my life. Date:29-7-2011 Place: LUCKNOW

Shalini

INDEX
TOPICS
CYTOGENETICS
Chromosome Preparation Karyotyping Giemsa Staining NOR Staining C-Banding

PAGE NO.

GENOTOXICITY TECHNIQUES
Comet Assay Micro Nuclei Test

MOLECULAR CYTOGENETICS
Genomic DNA isolation Agarose gel electrophoresis Ploymerase Chain Reaction (PCR)

ABBREVIATIONS

bp dNTPs g h l mA mM mg ml min M ng PCR rpm TBE UV V

Base Pair Deoxynucleoside triphosphate Gram(s) Hour(s) Micro liter Milli ampere Millimolar Milligram(s) Milliliter(s) Minute(s) Molar Nanogram(s) Polymerase Chain Reaction Rotation per minute Tris Borate EDTA Buffer Ultra Violet Volt(s)

TRAINING ON APPLICATIONS OF MOLECULAR CYTOGENETICS & GENOTOXICITY IN BIOTECHNOLOGY

SUMMER TRAINING REPORT SUBMITTED BY: SHALINI (09BIOTMCE028) B.TECH. BIOTECH. (MOLECULAR & CELLULAR ENGG.)

DEPARTMENT OF MOLECULAR CELLULAR ENGINEERING J.S.B.B SHIATS, ALLAHABAD 2011 UNDER THE GUIDANCE OF: DR.BASDEO KUSHWAHA (SENIOR SCIENTIST)

. NATIONAL BUREAU OF FISH GENETICS RESOURCES (INDIAN COUNCIL OF AGRICULTURAL RESEARCH) CANAL RING ROAD, P.O-DILKUSHA, TELIBAGH, LUCKNOW (U.P) INDIA