CM 4710, Biochemical Processes Fall 2007

Homework #7 Fri. 26 Oct., 2007

1. Batch Filtration: Cell Culture.

A Buchner funnel 8 cm in diameter is available for testing the filtration of a cell culture
suspension, which has a viscosity of 3.0 cp. The following data were obtained at a vacuum
pressure of 600 mm Hg applied to the funnel.

t (min) 26 96 197 342 537 692 989 (end)
V (ml) 100 200 300 400 500 600 692

The cell solids on the filter at the end of filtration were dried and found to weigh 14.0 g.
Determine the specific cake resistance and the medium Resistance r
m
. Then estimate how
long it would take to obtain 10,000 liters of filtrate from this cell culture on a filter with a
surface area of 10 m
2
and vacuum pressure of 500 mm Hg.




2. Continuous Centrifugation using a Tubular Centrifuge.

Yeast cells are recovered from a fermentation broth by using a tubular centrifuge. At a flow
rate of 12 l/min, the centrifuge must be operated at a rotation rate of 4000 rpm to collect the
cells. You are asked to predict operation under different conditions.

a) What rotation rate must you operate at if the flow rate is increased to 20 l/min?
b) If the length of the tubular portion of the centrifuge can be replaced, how much longer
must the length be if the flow rate is increased to 20 l/min?
c) What rotation rate must the unit in part a) operate at if the particle diameter is doubled?


Due Fri. 2 Nov., 2007
!lll !ensen volume L A L/vA v/A
PW7 (1) cm3 sec cm2
100 1360 30.26344 0.3103324 1.989438
Llme volume L/v Llme 200 3760 30.26344 0.37293828 3.978877
(s) (l) s/l (mln) 300 11820 30.26344 0.78383876 3.968313
1360 0.1 13600 26 400 20320 30.26344 1.02038193 7.937734
3760 0.2 28800 96 300 32220 30.26344 1.28199413 9.947192
11820 0.3 39400 197 600 41320 30.26344 1.37669142 11.93663
20320 0.4 31300 342 692 39340 30.26344 1.70397224 13.76691
32220 0.3 64440 337
41320 0.6 69200 692
39340 0.692 83731.44309 989
**1hls ls Lhe graph ploLLed Lhe way of Lhe oLher book l sLlll don'L geL Lhe
y = 1.1389L+03x + 3.2161L+03
8 = 9.9194L-01
0
10000
20000
30000
40000
30000
60000
70000
80000
90000
100000
0 0.1 0.2 0.3 0.4 0.3 0.6 0.7 0.8
L
/
v

(
s
/
l
)
v l (l)
y = 0.113x + 0.103
8 = 0.991
0
0.2
0.4
0.6
0.8
1
1.2
1.4
1.6
1.8
0 2 4 6 8 10 12 14 16
!
"
#
$
%
&
$"%
**1hls ls Lhe graph ploLLed Lhe way of Lhe oLher book - l sLlll don'L geL Lhe
slope LhaL Lhey do**
A = j*r
2
= 0.0030263 m
2
r = 4 cm 0.04 m
C = 14g/692ml = 0.02023 kg/l
= 3 cp 0.18 kg/(m*mln)
A = 600 mm Pg 79993.2 n/m
2
slope = 1/k 113890 sec/l
2
k = 1/slope 8.7804L-06 l
2
/sec
g
c
= 1
(kg*m)/(s
2
*n)
lnLercepL = 3216 s/l
o = slope*(2A
2
/C)*A*g
c
7.38313L+12 m/kg noLe: 1000 converLs l Lo m3, 60 converLs mln Lo s
r
m
= y lnL*k/2*a*C/A 6.9910L+11
m
-1
o = slope*(2A
2
/C)*A*g
c
7.38313L+12 m/kg A = 10
m
2
A = 300 mm Pg 66661 n/m
2
L=(*C*/2/A
2
//g
c
)[v
2
+2v(r
m
*A//C)] 3.81L+04 mln noLe: 1000 converLs l Lo m3, 60 converLs mln Lo s
967.93 hr
volume (l)