ACKNOWLEDGEMENT Firstly, I owe debt to God, the almighty by whose kindness I have been able to clear another chapter

of my life. I am grateful to Dr. P.K. Ghosh, Director, Central Salt & Marine Chemical Research Institute, Bhavnagar for providing me the facilities to work and administrative benediction. With a sense of gratitude, I earnestly wish to record my profound and heart thanks to my guide Dr. Jitendra Chikara, Discipline coordinator, Discipline of Waste land research, Central Salt & Marine Chemical Research Institute, Bhavnagar for his keen interest, guidance, constant encouragement and providing me with adequate facilities to complete this work in its wholeness. My heartfelt thanks to Mr. KG Vijay Anand, for his constant support and incessant encouragement throughout the course of this investigation. The learning experience under him helped me gain new insights in the field of Molecular Biology. I also regard my deep sense to Principal R. Krishnamurthy, and all the staff, C.G Bhakta Institute of Biotechnology, Veer Narmad South Gujarat University, with whose gratitude I have got the opportunity to do my dissertation work at CSMCRI, Gujarat. I also thank heart fully my lecturers who had supported me throughout my M.Sc study. My vocabulary falls short of words to express my deep sense of gratitude to my father Mr. Ashwinbhai Dhanani and mother Mrs. Rinalben Dhanani, for their positive attitude, infinity love and silent prayers. I also thank Mr. Shaik Gore Mastan, Mrs. Vachcha Batt, Mr. Ritesh Gautam, and Sarname singh for their co operation in doing my dissertation work at CSMCRI, Gujarat. I am unable to verbalize my pleasure merely by words to my all friends Dhudhiyo, JD, An Tinako, Buho, Bhabho, Goliyo and Sandy for their pleasant companionship and help throughout my study.

DHANANI HARIKRUSHNA

List of Abbreviation
DNA PVP CTAB TBE EDTA TE RFLP AFLP ITS RAPD Tris buffer min ml g mM M n bp v/v w/v ATP dNTPs PCR
0

- Deoxyribo Nucleic Acid - Polyvinyl Polypyrrolidone - Hexa decyl tri methyl Ammonium Bromide - Tris Borate EDTA - Ethylene diamine tetra acetic cid - Tris EDTA - Restriction Fragment Length Polymorphism - Amplified Fragment Length Polymorphism - Internal Transcribed Spacer - Random Amplified Polymorphic DNA - TRIS (Hydroxymethyl) Aminomethane - Minute - milliliter - Gram - milliMolar - Molar - nano - base pair - volume/volume - weight/volume - Adenosine Trinucleotide Phosphate - Deoxynucleotide Tri Phosphate - Micro - Polymerase Chain Reaction - Degree Celsius - Millimeter - Volts - Normal

mm V N

ii

INDEX
TOPICS
INTRODUCTION REVIEW OF LITERATURE MATERIALS AND METHODS RESULT SUMMARY REFERENCES ANNEXURE

PAGE NO.
1 4 19 28 31 31 38

iii