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Rac(G12V)
Hall, A. Science (1998) 279: 509514
Cdc42(G12V)
Effector
kcat = koff
kdiss = kon
3D Structures, invaluable for understanding the biochemistry and biology of your favorite system
X-Rays Crystal
Detector
Correct!!!!!!
P loop, GxxxxGKS/T, G1
P loop, GxxxxGKS/T, G1
Rab33
2008: > 400 deposits in the pdb data base 31 complexes with effectors 14 complexes with GEFs 8 complexes with GAPs 4 complexes with toxins 2011: >500 deposits
Ras-5p21
RhoA-1a2b
Arl2-1ksg
Rap-3rap
Cdc42-2qrz
Rab33B-1z06
Ras-5p21
RhoA-1a2b
Arl2-1ksg
Rap-3rap
Cdc42-2qrz
Rab33B-1z06
design: C.Kiel
Motor Protein
G Protein
N/TKxD, G4
sAk, G5
GDP
GTP
Control
Sample
- EDTA
RasGDP + [3H]GTP
Ras [3H]GTP
+ GDP
+ Sos
RasmGDP + GDP
RasGDP + mGDP
Multi-domain G Proteins
Conclusions
G proteins are universal switch molecules Their G domain has a typical , structure Work by a canonical switch mechanism Are specific for guanine nucleotides Have a slow intrinsic nucleotide exchange Have a slow intrinsic GTPase