ARF6

ARF6
ADP-ribosylation factor 6

PDB rendering based on 1e0s. Available structures PDB Ortholog search: PDBe [1], RCSB [2] List of PDB id codes 1E0S , 2A5D [14] 3PCR
[3] [4]

, 2A5F

[5]

, 2A5G

[6]

, 2BAO

[7]

, 2BAU

[8]

, 2J5X

[9]

, 2W83

[10]

, 3LVQ

[11]

, 3LVR

[12]

, 3N5C

[13]

Identifiers Symbols External IDs ARF6

[15]

;;
[16]

OMIM: 600464

MGI: 99435

[17]

HomoloGene: 1256

[18]

GeneCards: ARF6 Gene

[19]

ARF6

Gene Ontology Molecular function nucleotide binding [20] [21] GTPase activity [22] protein binding [23] GTP binding [24] thioesterase binding Cellular component ruffle [25] [26] intracellular [27] membrane fraction [28] cytoplasm [29] endosome [29] endosome [30] early endosome [31] Golgi apparatus [32] plasma membrane [33] cell cortex [34] endosome membrane [35] filopodium membrane [36] recycling endosome Biological process liver development [38] GTP catabolic process [39] apoptotic process [40] cellular component movement [41] cell adhesion [42] small GTPase mediated signal transduction [43] nervous system development [44] protein transport [45] vesicle-mediated transport [46] cell differentiation [47] positive regulation of actin filament polymerization [48] cortical actin cytoskeleton organization [49] ruffle organization [50] protein localization at cell surface [51] regulation of Rac protein signal transduction [52] negative regulation of receptor-mediated endocytosis [53] regulation of filopodium assembly [54] regulation of dendritic spine development [55] positive regulation of establishment of protein localization in plasma membrane Sources: Amigo
[56] [37]

/ QuickGO

[57]

RNA expression pattern

ARF6

More reference expression data Orthologs Species Entrez Ensembl UniProt RefSeq (mRNA) RefSeq (protein) Location (UCSC) Human 382
[59] [61]

[58]

Mouse 11845
[60] [62]

ENSG00000165527 P62330
[63] [65]

ENSMUSG00000044147 P62331
[64] [66]

NM_001663.3 NP_001654.1

NM_007481.3 NP_031507.1

[67]

[68]

Chr 14: [69] 50.36 50.36 Mb

[71]

Chr 12: [70] 70.47 70.48 Mb

[72]

PubMed search

ADP-ribosylation factor 6 (ARF6) is a member of the ADP ribosylation factor family of GTP-binding proteins. ARF6 has a variety of cellular functions that are frequently involved in trafficking of biological membranes and transmembrane protein cargo. ARF6 has specifically been implicated in endocytosis of plasma membrane proteins and also, to a lesser extent, plasma membrane protein recycling. This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7.[73] ARF6 can interact with arrestin upon receptor activation.

Interactions
Arf6 has been shown to interact with: Arrestin beta 1,[74] ARFIP2,[75][76] EXOC5,[77] and KIAA0090,[78] Muscarinic acetylcholine receptor M3.[79]

ARF6

References

[1] http:/ / www. ebi. ac. uk/ pdbe/ searchResults. html?display=both& term=Q6FGZ2%20or%20P62330%20or%20E2RHL4%20or%20P62331%20or%20Q3U0D7%20or%20P62332%20or%20Q6P2U5%20or%20Q6P2U5%20or%20 [2] http:/ / www. rcsb. org/ pdb/ search/ smartSubquery. do?smartSearchSubtype=UpAccessionIdQuery& accessionIdList=Q6FGZ2,P62330,E2RHL4,P62331,Q3U0D7,P62332,Q6P2U5,Q6P2U5,P40946,Q7QII7,Q9U2S4,Q9Y7Z2,P40994,Q6CTD4,Q75CJ7,G4N9S6,Q [3] http:/ / www. rcsb. org/ pdb/ cgi/ explore. cgi?pdbId=1E0S [4] http:/ / www. rcsb. org/ pdb/ cgi/ explore. cgi?pdbId=2A5D [5] http:/ / www. rcsb. org/ pdb/ cgi/ explore. cgi?pdbId=2A5F [6] http:/ / www. rcsb. org/ pdb/ cgi/ explore. cgi?pdbId=2A5G [7] http:/ / www. rcsb. org/ pdb/ cgi/ explore. cgi?pdbId=2BAO [8] http:/ / www. rcsb. org/ pdb/ cgi/ explore. cgi?pdbId=2BAU [9] http:/ / www. rcsb. org/ pdb/ cgi/ explore. cgi?pdbId=2J5X [10] http:/ / www. rcsb. org/ pdb/ cgi/ explore. cgi?pdbId=2W83 [11] http:/ / www. rcsb. org/ pdb/ cgi/ explore. cgi?pdbId=3LVQ [12] http:/ / www. rcsb. org/ pdb/ cgi/ explore. cgi?pdbId=3LVR [13] http:/ / www. rcsb. org/ pdb/ cgi/ explore. cgi?pdbId=3N5C [14] http:/ / www. rcsb. org/ pdb/ cgi/ explore. cgi?pdbId=3PCR [15] http:/ / www. genenames. org/ data/ hgnc_data. php?hgnc_id=659 [16] http:/ / omim. org/ entry/ 600464 [17] http:/ / www. informatics. jax. org/ searches/ accession_report. cgi?id=MGI:99435 [18] http:/ / www. ncbi. nlm. nih. gov/ entrez/ query. fcgi?cmd=Retrieve& db=homologene& dopt=HomoloGene& list_uids=1256 [19] [20] [21] [22] [23] [24] [25] [26] [27] [28] [29] [30] [31] [32] [33] [34] [35] [36] [37] [38] [39] [40] [41] [42] [43] [44] [45] [46] [47] [48] [49] [50] [51] [52] http:/ / www. genecards. org/ cgi-bin/ carddisp. pl?id_type=entrezgene& id=382 http:/ / amigo. geneontology. org/ cgi-bin/ amigo/ go. cgi?view=details& search_constraint=terms& depth=0& query=GO:0000166 http:/ / amigo. geneontology. org/ cgi-bin/ amigo/ go. cgi?view=details& search_constraint=terms& depth=0& query=GO:0003924 http:/ / amigo. geneontology. org/ cgi-bin/ amigo/ go. cgi?view=details& search_constraint=terms& depth=0& query=GO:0005515 http:/ / amigo. geneontology. org/ cgi-bin/ amigo/ go. cgi?view=details& search_constraint=terms& depth=0& query=GO:0005525 http:/ / amigo. geneontology. org/ cgi-bin/ amigo/ go. cgi?view=details& search_constraint=terms& depth=0& query=GO:0031996 http:/ / amigo. geneontology. org/ cgi-bin/ amigo/ go. cgi?view=details& search_constraint=terms& depth=0& query=GO:0001726 http:/ / amigo. geneontology. org/ cgi-bin/ amigo/ go. cgi?view=details& search_constraint=terms& depth=0& query=GO:0005622 http:/ / amigo. geneontology. org/ cgi-bin/ amigo/ go. cgi?view=details& search_constraint=terms& depth=0& query=GO:0005624 http:/ / amigo. geneontology. org/ cgi-bin/ amigo/ go. cgi?view=details& search_constraint=terms& depth=0& query=GO:0005737 http:/ / amigo. geneontology. org/ cgi-bin/ amigo/ go. cgi?view=details& search_constraint=terms& depth=0& query=GO:0005768 http:/ / amigo. geneontology. org/ cgi-bin/ amigo/ go. cgi?view=details& search_constraint=terms& depth=0& query=GO:0005769 http:/ / amigo. geneontology. org/ cgi-bin/ amigo/ go. cgi?view=details& search_constraint=terms& depth=0& query=GO:0005794 http:/ / amigo. geneontology. org/ cgi-bin/ amigo/ go. cgi?view=details& search_constraint=terms& depth=0& query=GO:0005886 http:/ / amigo. geneontology. org/ cgi-bin/ amigo/ go. cgi?view=details& search_constraint=terms& depth=0& query=GO:0005938 http:/ / amigo. geneontology. org/ cgi-bin/ amigo/ go. cgi?view=details& search_constraint=terms& depth=0& query=GO:0010008 http:/ / amigo. geneontology. org/ cgi-bin/ amigo/ go. cgi?view=details& search_constraint=terms& depth=0& query=GO:0031527 http:/ / amigo. geneontology. org/ cgi-bin/ amigo/ go. cgi?view=details& search_constraint=terms& depth=0& query=GO:0055037 http:/ / amigo. geneontology. org/ cgi-bin/ amigo/ go. cgi?view=details& search_constraint=terms& depth=0& query=GO:0001889 http:/ / amigo. geneontology. org/ cgi-bin/ amigo/ go. cgi?view=details& search_constraint=terms& depth=0& query=GO:0006184 http:/ / amigo. geneontology. org/ cgi-bin/ amigo/ go. cgi?view=details& search_constraint=terms& depth=0& query=GO:0006915 http:/ / amigo. geneontology. org/ cgi-bin/ amigo/ go. cgi?view=details& search_constraint=terms& depth=0& query=GO:0006928 http:/ / amigo. geneontology. org/ cgi-bin/ amigo/ go. cgi?view=details& search_constraint=terms& depth=0& query=GO:0007155 http:/ / amigo. geneontology. org/ cgi-bin/ amigo/ go. cgi?view=details& search_constraint=terms& depth=0& query=GO:0007264 http:/ / amigo. geneontology. org/ cgi-bin/ amigo/ go. cgi?view=details& search_constraint=terms& depth=0& query=GO:0007399 http:/ / amigo. geneontology. org/ cgi-bin/ amigo/ go. cgi?view=details& search_constraint=terms& depth=0& query=GO:0015031 http:/ / amigo. geneontology. org/ cgi-bin/ amigo/ go. cgi?view=details& search_constraint=terms& depth=0& query=GO:0016192 http:/ / amigo. geneontology. org/ cgi-bin/ amigo/ go. cgi?view=details& search_constraint=terms& depth=0& query=GO:0030154 http:/ / amigo. geneontology. org/ cgi-bin/ amigo/ go. cgi?view=details& search_constraint=terms& depth=0& query=GO:0030838 http:/ / amigo. geneontology. org/ cgi-bin/ amigo/ go. cgi?view=details& search_constraint=terms& depth=0& query=GO:0030866 http:/ / amigo. geneontology. org/ cgi-bin/ amigo/ go. cgi?view=details& search_constraint=terms& depth=0& query=GO:0031529 http:/ / amigo. geneontology. org/ cgi-bin/ amigo/ go. cgi?view=details& search_constraint=terms& depth=0& query=GO:0034394 http:/ / amigo. geneontology. org/ cgi-bin/ amigo/ go. cgi?view=details& search_constraint=terms& depth=0& query=GO:0035020 http:/ / amigo. geneontology. org/ cgi-bin/ amigo/ go. cgi?view=details& search_constraint=terms& depth=0& query=GO:0048261

[53] http:/ / amigo. geneontology. org/ cgi-bin/ amigo/ go. cgi?view=details& search_constraint=terms& depth=0& query=GO:0051489 [54] http:/ / amigo. geneontology. org/ cgi-bin/ amigo/ go. cgi?view=details& search_constraint=terms& depth=0& query=GO:0060998 [55] http:/ / amigo. geneontology. org/ cgi-bin/ amigo/ go. cgi?view=details& search_constraint=terms& depth=0& query=GO:0090004

ARF6
[56] http:/ / amigo. geneontology. org/ cgi-bin/ amigo/ gp-assoc. cgi?gp=UniProtKB:P62330 [57] http:/ / www. ebi. ac. uk/ QuickGO/ GProtein?ac=P62330 [58] http:/ / biogps. org/ gene/ 382/ [59] http:/ / www. ncbi. nlm. nih. gov/ entrez/ query. fcgi?db=gene& cmd=retrieve& dopt=default& list_uids=382& rn=1 [60] http:/ / www. ncbi. nlm. nih. gov/ entrez/ query. fcgi?db=gene& cmd=retrieve& dopt=default& list_uids=11845& rn=1 [61] http:/ / www. ensembl. org/ Homo_sapiens/ geneview?gene=ENSG00000165527;db=core [62] http:/ / www. ensembl. org/ Mus_musculus/ geneview?gene=ENSMUSG00000044147;db=core [63] http:/ / www. uniprot. org/ uniprot/ P62330 [64] http:/ / www. uniprot. org/ uniprot/ P62331 [65] http:/ / www. ncbi. nlm. nih. gov/ entrez/ viewer. fcgi?val=NM_001663. 3 [66] http:/ / www. ncbi. nlm. nih. gov/ entrez/ viewer. fcgi?val=NM_007481. 3 [67] http:/ / www. ncbi. nlm. nih. gov/ entrez/ viewer. fcgi?val=NP_001654. 1 [68] http:/ / www. ncbi. nlm. nih. gov/ entrez/ viewer. fcgi?val=NP_031507. 1 [69] http:/ / genome. ucsc. edu/ cgi-bin/ hgTracks?org=Human& db=hg19& position=chr14:50359810-50361490 [70] http:/ / genome. ucsc. edu/ cgi-bin/ hgTracks?org=Mouse& db=mm9& position=chr12:70473137-70476966 [71] http:/ / www. ncbi. nlm. nih. gov/ sites/ entrez?db=gene& cmd=Link& LinkName=gene_pubmed& from_uid=382 [72] http:/ / www. ncbi. nlm. nih. gov/ sites/ entrez?db=gene& cmd=Link& LinkName=gene_pubmed& from_uid=11845 [73] "Entrez Gene: ARF6 ADP-ribosylation factor 6" (http:/ / www. ncbi. nlm. nih. gov/ sites/ entrez?Db=gene& Cmd=ShowDetailView& TermToSearch=382). . [74] Claing A, Chen W, Miller WE, Vitale N, Moss J, Premont RT, Lefkowitz RJ (November 2001). "beta-Arrestin-mediated ADP-ribosylation factor 6 activation and beta 2-adrenergic receptor endocytosis". J. Biol. Chem. 276 (45): 4250913. doi:10.1074/jbc.M108399200. PMID11533043. [75] D'Souza-Schorey C, Boshans RL, McDonough M, Stahl PD, Van Aelst L (September 1997). "A role for POR1, a Rac1-interacting protein, in ARF6-mediated cytoskeletal rearrangements". EMBO J. 16 (17): 544554. doi:10.1093/emboj/16.17.5445. PMC1170175. PMID9312003. [76] Shin OH, Exton JH (August 2001). "Differential binding of arfaptin 2/POR1 to ADP-ribosylation factors and Rac1". Biochem. Biophys. Res. Commun. 285 (5): 126773. doi:10.1006/bbrc.2001.5330. PMID11478794. [77] Prigent M, Dubois T, Raposo G, Derrien V, Tenza D, Ross C, Camonis J, Chavrier P (December 2003). "ARF6 controls post-endocytic recycling through its downstream exocyst complex effector". J. Cell Biol. 163 (5): 111121. doi:10.1083/jcb.200305029. PMC2173613. PMID14662749. [78] Prieto C, De Las Rivas J (July 2006). "APID: Agile Protein Interaction DataAnalyzer" (http:/ / bioinfow. dep. usal. es/ apid/ html/ home. htm). Nucleic Acids Res. 34 (Web Server issue): W298302. doi:10.1093/nar/gkl128. PMC1538863. PMID16845013. . [79] Mitchell R, Robertson DN, Holland PJ, Collins D, Lutz EM, Johnson MS (September 2003). "ADP-ribosylation factor-dependent phospholipase D activation by the M3 muscarinic receptor". J. Biol. Chem. 278 (36): 3381830. doi:10.1074/jbc.M305825200. PMID12799371.

Further reading
Sabe H (2004). "Requirement for Arf6 in cell adhesion, migration, and cancer cell invasion". J. Biochem. 134 (4): 4859. doi:10.1093/jb/mvg181. PMID14607973. Joseph AM, Kumar M, Mitra D (2005). "Nef: "necessary and enforcing factor" in HIV infection". Curr. HIV Res. 3 (1): 8794. doi:10.2174/1570162052773013. PMID15638726. Tsuchiya M, Price SR, Tsai SC, et al. (1991). "Molecular identification of ADP-ribosylation factor mRNAs and their expression in mammalian cells". J. Biol. Chem. 266 (5): 27727. PMID1993656. Stearns T, Willingham MC, Botstein D, Kahn RA (1990). "ADP-ribosylation factor is functionally and physically associated with the Golgi complex". Proc. Natl. Acad. Sci. U.S.A. 87 (3): 123842. doi:10.1073/pnas.87.3.1238. PMC53446. PMID2105501. D'Souza-Schorey C, Stahl PD (1995). "Myristoylation is required for the intracellular localization and endocytic function of ARF6". Exp. Cell Res. 221 (1): 1539. doi:10.1006/excr.1995.1362. PMID7589240. D'Souza-Schorey C, Li G, Colombo MI, Stahl PD (1995). "A regulatory role for ARF6 in receptor-mediated endocytosis". Science 267 (5201): 11758. doi:10.1126/science.7855600. PMID7855600. Welsh CF, Moss J, Vaughan M (1995). "ADP-ribosylation factors: a family of approximately 20-kDa guanine nucleotide-binding proteins that activate cholera toxin". Mol. Cell. Biochem. 138 (1-2): 15766. doi:10.1007/BF00928458. PMID7898460. Amor JC, Harrison DH, Kahn RA, Ringe D (1995). "Structure of the human ADP-ribosylation factor 1 complexed with GDP". Nature 372 (6507): 7048. doi:10.1038/372704a0. PMID7990966.

ARF6 Orcl L, Palmer DJ, Amherdt M, Rothman JE (1993). "Coated vesicle assembly in the Golgi requires only coatomer and ARF proteins from the cytosol". Nature 364 (6439): 7324. doi:10.1038/364732a0. PMID8355790. Helms JB, Palmer DJ, Rothman JE (1993). "Two distinct populations of ARF bound to Golgi membranes". J. Cell Biol. 121 (4): 75160. doi:10.1083/jcb.121.4.751. PMC2119793. PMID8491770. Cavenagh MM, Whitney JA, Carroll K, et al. (1996). "Intracellular distribution of Arf proteins in mammalian cells. Arf6 is uniquely localized to the plasma membrane". J. Biol. Chem. 271 (36): 2176774. doi:10.1074/jbc.271.36.21767. PMID8702973. D'Souza-Schorey C, Boshans RL, McDonough M, et al. (1997). "A role for POR1, a Rac1-interacting protein, in ARF6-mediated cytoskeletal rearrangements". EMBO J. 16 (17): 544554. doi:10.1093/emboj/16.17.5445. PMC1170175. PMID9312003. Frank S, Upender S, Hansen SH, Casanova JE (1998). "ARNO is a guanine nucleotide exchange factor for ADP-ribosylation factor 6". J. Biol. Chem. 273 (1): 237. doi:10.1074/jbc.273.1.23. PMID9417041. Yang CZ, Heimberg H, D'Souza-Schorey C, et al. (1998). "Subcellular distribution and differential expression of endogenous ADP-ribosylation factor 6 in mammalian cells". J. Biol. Chem. 273 (7): 400611. doi:10.1074/jbc.273.7.4006. PMID9461590. Mao M, Fu G, Wu JS, et al. (1998). "Identification of genes expressed in human CD34(+) hematopoietic stem/progenitor cells by expressed sequence tags and efficient full-length cDNA cloning". Proc. Natl. Acad. Sci. U.S.A. 95 (14): 817580. doi:10.1073/pnas.95.14.8175. PMC20949. PMID9653160. Andreev J, Simon JP, Sabatini DD, et al. (1999). "Identification of a new Pyk2 target protein with Arf-GAP activity". Mol. Cell. Biol. 19 (3): 233850. PMC84026. PMID10022920. Radhakrishna H, Al-Awar O, Khachikian Z, Donaldson JG (1999). "ARF6 requirement for Rac ruffling suggests a role for membrane trafficking in cortical actin rearrangements". J. Cell. Sci. 112 (6): 85566. PMID10036235. Kim HS (1999). "Assignment of the human ADP-ribosylation factor 6 (ARF6) gene to chromosome 7q22.1 by radiation hybrid mapping". Cytogenet. Cell Genet. 84 (1-2): 94. doi:10.1159/000015225. PMID10343114. Langille SE, Patki V, Klarlund JK, et al. (1999). "ADP-ribosylation factor 6 as a target of guanine nucleotide exchange factor GRP1". J. Biol. Chem. 274 (38): 27099104. doi:10.1074/jbc.274.38.27099. PMID10480924. Honda A, Nogami M, Yokozeki T, et al. (1999). "Phosphatidylinositol 4-phosphate 5-kinase alpha is a downstream effector of the small G protein ARF6 in membrane ruffle formation". Cell 99 (5): 52132. doi:10.1016/S0092-8674(00)81540-8. PMID10589680.

External links
MeSH ARF6+protein,+human (http://www.nlm.nih.gov/cgi/mesh/2011/MB_cgi?mode=&term=ARF6+ protein,+human) Ikeda S, Ushio-Fukai M, Zuo L, et al. (2005). "Novel role of ARF6 in vascular endothelial growth factor-induced signaling and angiogenesis". Circ. Res. 96 (4): 46775. doi:10.1161/01.RES.0000158286.51045.16. PMID15692085.

Article Sources and Contributors

Article Sources and Contributors

ARF6 Source: http://en.wikipedia.org/w/index.php?oldid=470699156 Contributors: AndrewGNF, Arcadian, Boghog, Brianf711, Bryhall, Creidieki, D-rew, Eequor, Jag123, Jfdwolff, Keenan Pepper, Mushin, Rich Farmbrough, Rjwilmsi, StudierMalMarburg, Yeast2Hybrid, 2 anonymous edits

Image Sources, Licenses and Contributors

file:Protein_ARF6_PDB_1e0s.png Source: http://en.wikipedia.org/w/index.php?title=File:Protein_ARF6_PDB_1e0s.png License: Creative Commons Attribution-Sharealike 3.0 Contributors: Emw file:PBB_GE_ARF6_203311_s_at_tn.png Source: http://en.wikipedia.org/w/index.php?title=File:PBB_GE_ARF6_203311_s_at_tn.png License: GNU Free Documentation License Contributors: file:PBB_GE_ARF6_203312_x_at_tn.png Source: http://en.wikipedia.org/w/index.php?title=File:PBB_GE_ARF6_203312_x_at_tn.png License: GNU Free Documentation License Contributors: -

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