Title: Short training course Duration: One month training programme, Training Fee: 10000/Scheduled time of start: May-July

and December-February (Every Year)

S. No. 1. 2.

Topic Introduction to the Department of Agricultural Biotechnology and acquaintance with different labs and green houses of the department

Probable days 1

Familiarization with handling and operations of various important equipments in 2 the labs, such as autoclaves, laminar Air flow systems, centrifuges, ultra centrifuges, different types of PCR machines, electrophoresis units, Gel documentation systems, HPLCs, Fluorescent microscope, etc. Planting of different seeds for DNA extraction under green house conditions and preparing chemicals and glassware for DNA extraction Importance of Tissue culture in Agriculture (theory) and Preparing glassware (washing, cleaning and autoclaving) for tissue culture experiments preparation of stock solutions for tissue culture media preparation Tissue Culture media preparations and preparation of explants for different tissue culture experiments like, micro propagation, callus culture, cell cultures, embryo culture etc.(Theory and practicals) Sub culturing of tissue culture cultures etc. PCR and its applications in agriculture DNA extraction, quantification and assessment of its quality Recombinant DNA technology and applications in development of transgenic (theory) Fluorescent In situ hybridization (FISH) Genomic In situ hybridization (GISH) - Theory Different equipments and protocols for FISH and GISH, EDA etc. Preparing report (only computer facility will be provided no printing facility) 2 6 4 8

3. 4. 5. 6.

7. 8. 9. 10.

2 2 2 1

11.

Title: Short training course Duration: Two months training programme, Training Fee: `20000/Scheduled time of start: May-July and December-February (Every Year)

S. No. 1. 2.

Topic Introduction to the Department of Agricultural Biotechnology and acquaintance with different labs and green houses of the department

Probable days 1

Familiarization with handling and operations of various important equipments in 2 the labs, such as autoclaves, laminar Air flow systems, centrifuges, ultra centrifuges, different types of PCR machines, electrophoresis units, Gel documentation systems, HPLCs, Fluorescent microscope, etc. Planting of different seeds for DNA extraction under green house conditions and preparing chemicals and glassware for DNA extraction 2

3. 4. 5. 6.

Importance of Tissue culture in Agriculture (theory) and preparation of stock 2 solutions for tissue culture media preparation Preparing glasswares (washing, cleaning and autoclaving) for tissue culture 2 experiments Tissue Culture media preparations and preparation of explants for different tissue culture experiments like, micro propagation, callus culture, cell cultures, embryo culture etc. Sub culturing of tissue culture cultures PCR and its application in agriculture (Theory) Equipments and chemicals for different molecular markers DNA extraction, quantification and assessment of its quality Analysis of PCR products through agarose gel Recording data for different markers and analyzing data using different software Recombinant DNA technology and applications in development of transgenic (theory) Multiplication of E coli stains to be used for production of transgenic Fluorescent In situ hybridization (FISH) Genomic In situ hybridization (GISH) - Theory Different equipments and protocols for FISH and GISH, EDA etc. Washing and cleaning of glassware for slide preparation Fixing and preparing slides for FISH and GISH Preparing report (only computer facility will be provided no printing facility) 4

7. 8. 9. 10. 11. 12. 13.

2 4 8 8 2 4 3

14. 15. 16.

3 4 2

Title: Long training course Duration: Four-six months training programme, Training Fee: `10000/month Scheduled time of start: Throughout the year

S.no. 1. 2.

Topic Introduction to the Department of Agricultural Biotechnology and Acquaintance with different labs of the department Operations and mechanism of different equipments in labs, such as centrifuges, ultra centrifuges, different types of PCR machines, electrophoresis units, Gel documentation systems, HPLCs, Fluorescent microscope, etc. Importance of Tissue culture in Agriculture (theory) and preparation of stock solutions for tissue culture Planting different seeds material under green house conditions for DNA extraction and preparing stock solutions and glassware for DNA extraction Preparing glassware (washing, cleaning and autoclaving) for tissue culture experiments Preparing material for different tissue culture experiments like, micro propagation, callus culture, cell cultures, embryo culture, anther culture etc. Sub culturing of tissue cultures, transferring callus on rooting and shooting media Hardening and transferring plantlets to soils Colchicine treatment to haploid plants for producing Doubled Haploids Molecular markers and their role in agriculture (Theory),

Probable days 1 2

3. 4. 5. 6. 7. 8. 9. 10 . 11 . 12 . 13 . 14 . 15 . 16 . 17 . 18 . 19 . 20 . 21 .

2 2 2 4 4-10 4-6 4-6 1

Equipments and chemicals required for different molecular markers, washing and autoclaving of glassware for molecular experiments Plant DNA extraction, PCR and gels Electrophoresis (PAGE, agarose)

15-20

Development of PCR-based DNA markers like SSRs (Theory) and SNPs + in silico mining of EST, SSRs etc Introduction of Bioinformatics- primer designing etc.

30-40

5-10

Recording of data for different markers and analyzing data using different software

5-10

Transgenic (theory) Equipments and chemical requirements for the development of transgenic plants Fluorescent In situ hybridization (FISH) Genomic In situ hybridization (GISH) (Theory) Different equipments and protocols for FISH and GISH, EDA etc. Washing, cleaning and autoclaving of glassware for cytology work

2-5

Fixing material for slides to be used for FISH and GISH

5-10

Preparing slides for FISH

Preparing report

OR Title: Long training course Duration: Four-six months training programme, Training Fee: `10000/month Scheduled time of start: Throughout the year The candidate undergoing 4-6 months training shall be assigned short term research project in the areas of plant tissue culture like, development of tissue culture protocols for economically important plant, anther culture for speedy development of Doubled Haploids and DNA marker technology for diversity analysis, gene mapping, markers assisted pyramids of genes for biotic and abiotic stresses etc.

Note: 1. 2. 3. 4. 5. The duration of training module shall be 1-6 months and the candidate has to specify clearly the training duration in which he/she will be joining. Imparting of the aforesaid training is not a regular feature. Candidates will be selected on basis of first-cum-first-serve basis and the maximum number of trainees to be accommodated at a time will in no case exceed twenty. Training will be imparted during March to June every year subject to the availability of space in the Lab and time with the In-charge of this Lab. For undertaking training, the students should apply in the prescribed format(Annexure I) through their respective Principal/Dean/Registrar to the Registrar, CSKHPKV,Palampur176062 H.P. well in time who will forward the same to the concerned Head of the Department for further action. All subsequent correspondences to be made with the concerned Head of the Department. The trainee shall be required to pay the entire training fee at the start of the training. For preparing report of the training it is the whole responsibility of the candidates and only the guidance will be provided. No additional computer facility and printing facility will be provided by the department. The Department reserves the right to decline /discontinue the training on account of unreasonable conduct of a trainee

6. 7. 8.

For further inquiry, following can be contacted: Dr. R.K.Chahota Training Organizer Mobile. No. : 09418369800 Email: rkchahota@yahoo.com

Dr. R.K.Kapila Associate Professor Mobile No.:09418101452 Email :rakeshkapila@hillagric.ernet.in

Annexure-1

CSK Himachal Pradesh Agriculture University, Palampur,H.P. Department of Agricultural Biotechnology

1. 2. Name of the Training Bio-data Name Mothers Name Fathers Name : : : : :

Date of Birth : Academic Qualifications ( Matric onward): Qualification Year of passing Marks (%OCPA)

Institute

University/Board

3.

Present Status Class Registration No. Roll No. Institute University

: : : : : : :

4. 5.

Permanent Address

Correspondence Address : ( with Tel. No./Mobile No. and E-mail): (Signature of the student )

Recommended by: (Major Advisor ) (Principal/Dean/ Registrar) with seal

Note : The application with supporting documents should be sent to the Registrar CSK HPKV Palampur 176062 (HP)